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A Comparison of Bird Abundance in Different Types of Rural Settlements along the Czech-Austrian Border
Třeská, Klára ; Zasadil, Petr (advisor) ; Hodačová, Lenka (referee)
Abstract
Along with the changes in the management of human, which took place from the mid-20th century, bird populations changed the balances. The aim of my thesis is to determine the differences in the abundance and distribution of selected species of synanthropic birds. These concern of the following species: House sparrow (Passer domesticus), Tree sparrow (Passer montanus), Collared dove (Sreptopelia decaocto), Common linnet (Carduelis cannabina), White wagtail (Motacilla alba), Common chaffinch (Fringilla coelebs), Black redstart (Phoenicurus ochruros), European goldfinch (Carduelis carduelis), Common starling (Sturnus vulgaris), European greenfinch (Carduelis chloris) and European serin (Serinus serinus). Using the simplified method of mapping (Bibby et al. 1992), the bird census took place in spring 2015 in 20 villages on the Czech-Austrian border. Counting squares were set in each village for different types of environment: the center of the village, edge of village and agricultural area including farm building with livestock.
The results confirm the dependence of house sparrow to agricultural areas with breeding livestock in the Czech Republic. Abundance, density and frequency was evaluate for each species separately. Effect of biotope and state were significant effects only for House sparrow population. Factors of poultry and built up area were significant for White wagtail bird. Effect of state was significant for bird species Collared dove. There were no effects for Tree sparrows populations.
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The renovation of historical parks near the small feudal residences on the example of the study of the renovation of the castle garden in Doudleby nad Orlici in Eastern Bohemia.
Faltysová, Lenka ; Buttry, Ivana (advisor) ; Jakub, Jakub (referee)
The aim of this work is the processing methodology and presentation process how to restore monuments of garden art. On the example of the revitalization of the castle garden immovable cultural monument Castle Doudleby nad Orlicí, based on the study of literature, similar interventions and the analysis and interpretation of various types of archival material the current quality of historic garden was evaluated. It is based on field survey, which consists of dendrological research, analyzing the authenticity of trees and appreciation of all the collected documents, historical sources and professional literature. Concept recovery of castle garden is based on the expert authority´s binding point of view of the state care of historical monuments.
Renaissance castle in Doudleby nad Orlici is located in the eastern part of the town on a slightly raised hill on the right bank of the River Divoká Orlice. There was a wooden medieval fortress with the farmyard on this place in the 13th century. Sr Mikuláš from Bubno started to build a hunting lodge in the Renaissance style as villa in 1588, which was finished in 1590. The significance of Renaissance residence, which complement the early Baroque elements from the late 17th century, is enhanced by the opulent and impressive sgraffito - carpet pattern covering all external and internal frontage, restored in 1886 by Kirchner. The tall chimneys are the part of decoration in the courtyard decorated with sgraffito as well. The chateau complex is formed along its circuit of farm buildings and residential buildings. Building of castle itself is separated by a park with old trees and newer lawns (Hieke, 1984).
Castle park in Doudleby nad Orlicí was founded in 1809 in compositional continuity of the Renaissance castle building. The park forms one organic complex with the castle. The promoter strived, like in the castle rooms, to demonstrate here his wealth, his sense of art and his botanical-dendrological knowledge. The park is designed as a narrow disposition in landscape style and it is composed as a long vista to the castle. The area of the landscape park is 3.76 hectares nowadays.
The literature search is especially dedicated to the Renaissance and its relationship to the garden art. The monument care in the Czech Republic and the activity of professional organization of the monument care of the National Heritage Institute in Josefov is introduced in this section.
The methodological guide describes the used bolsterings and resulting outputs on the basis of them are implemented of such measures, which will help to improve the health and vitality of existing promising trees and new plantings of trees, which are consistent with the original composition with emphasis on the rehabilitation and upgrading of historical value of the object. Treatment of trees and supplementary planting will be done within the frame of total revitalization of the castle park.
The practical unit describes the current status of vegetation monuments of garden art. The inventory trees is the essential tool for analyzing the current status of vegetation monuments of garden art. Inventory, which should provide quality base for a draft recovery, should also be addressed in certain parts with characteristics of individual species (Krejčiřík, 2015).
The results of field investigation were completely recorded in the graphical output.
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Abundance of house sparrow (Passer domesticus) and other synantropic species in small settlements around Prague
Řezáčová, Andrea ; Šmejdová, Lucie (advisor) ; Zasadil, Petr (referee)
In the surroundings of Prague, as well as in many other parts of Europe the population numbers of synanthropic species were reduced in recent years. These changes in abundance are attributed to the change of use of agricultural landscapes, the loss of small poultry farms and mainly a change in the character of the construction. The aim of this thesis was to ascertain the abundance of selected species of synanthropic birds and compared their numbers with the data obtained in 2012. In this study were also evaluated differences in the abundance of these species in the localities of old construction area and new construction area. There were selected 3 main leaders, house sparrow (Passer domesticus), tree sparrow (Passer montanus) and eurasian collared dove (Streptopelia decaocto) from all monitored species for the statistical processing of the results. Census was carried out in a total of 40 squares, 20 of which was located in an old construction area and 20 in the construction of the new. All of the site lies in an area with a high degree of suburbanization in the central region an in around of Prague. The study
area covered an area of 200 x 200 meters and were visited a total of twice during the nesting period in the spring of 2015. The results show the preferences of the old buildings for the house sparrow and the eurasian collared dove. Tree sparrow was more numerous in the localities, characterizing the new housing development. For eurasion collared dove was observed increasing trend in abundance, while in both species of sparrows was recorded its decline. The most numerous species was the house sparrow with a total of 209 individuals, on the contrary, at least individuals, 23, was noted for european serin (Serinus serinus).
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Winter Density of House Sparrow in Different types of Rural Settlements along the Czech – Austria Border
Oberpfalzerová, Michaela ; Zasadil, Petr (advisor) ; Šmejdová, Lucie (referee)
From the point of view of the Czech Republic in the last 80 years we can specify two periods, which had a significant impact on the general character and development in agriculture. The first period can be traced to the 50s of the last century, in which the concentration of agricultural production headed towards to factory farming. The second one began at the beginning of the 90s. At that time a sharp downtown of animal production occurred and the sooner concentrated production began, after the regaining by previous owners, to erode into what it is today private agriculture.
This thesis aims tocontribute to a confirmation of rejection of the hypothesis that the above described changes have a negative impact on the selected avian species. These species are represented by significantsynanthropic birds: house sparrow (which is a crucial representative in this thesis), tree sparrow and collared dove. Thirty municipalities were chosen to emphasize the differences in quantity of these species, half of them located on each side of the border with the Czech Republic and Austria. The comparison of quantities of bird populations took place in the surroundings of the cities Znojmo and Retz.In this thesis the Republic of Austria acts as a country which has not been burdened by a substantial economic development and which has applied the continuous market economy since the end of the Second World War.
In the individual squares the numbers of the representatives of the studied species and the environmental characteristics were recorded during the winter period. The significant differences in the numbers of birds at selected locations were demonstrated using a multifactor ANOVA STATISTICA 12. Moreover, the impact of selected environmental variables on the abundance of observed species was also analysed. It was found that the selected species depend on an inclusion into the individual states as well as on a classification into various biotope types. Finally, the dependence on the area of trees and bushes, the proportion of new buildings and the presence of poultry in the square were also observed. The most numerous species in the Republic of Austria was the house sparrow, the most abundant species in the Czech Republic was the collared dove. A slight increase in the occurrence of the collared dove was recorded in both states. Tree sparrow as a species occurred in relatively large numbers, but only locally. It was found that the populationsof the above mentioned species were generally more numerous in the Austrian municipalities. An interesting fact is that most of the observed individuals in the Czech Republic occurred in village built-up areas rather than in agricultural premises, as it was initially estimated.
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Molecular detection RYR1 gene mutation causing malignant hyperthermia in dogs
Krausová, Klára ; Vejl, Pavel (advisor) ; Melounová, Martina (referee)
The theme of this work are methods for molecular detection of mutations in the dog genome causing malignant hyperthermia.
The work is divided into two main parts. The first part is a literary research summarizing knowledge about malignant hyperthermia from the human and veterinary anesthesiology molecular biology and genetics. The second part of the work describes the methodology and conclusions drawn from the molecular detection of above mentioned mutation which was conducted in the laboratory of the Department of genetics.
The literary research deal with malignant hyperthermia syndrome. The aim is to familiarize with symptomatic manifestations triggers the mechanisms the development of acute malignant hyperthermia and its clinical diagnosis. The first cases of observation of malignant hyperthermia syndrome are also described as well as the history of the research which led to a detailed description of this disease in humans dogs and other species bearing malignant hyperthermia causing mutation.
The next part of literary research is a summary of knowledge about the mechanism of mutation the detection of sequences containing this mutation and influence on the arrangement of proteins in the ryanodine receptors.
For the last part of literary research commonly used laboratory techniques of non invasive mutation detection were chosen. These techniques are mostly used by accredit genetics laboratories. Listing of Czech laboratories performing diagnostic of gene RYR1 mutation is also contained.
Experimental part of barchelors thesis is focussed on molecular marker optimalization for casual mutation in RYR1 gene detection by technique of restriction fragment length polymorphism (PCR RFLP). This technique was apply to 338 deputies of different dog breeds. Accomplished analysis did not confirm presence of the mutated allele in assessed breeds population. Result of PCR RFLP technique was confirmed by sequence analysis.
Results were discussed with literary sources which did not judge real breeds but are developing a model special bred line with presumed mutated allele segregation. The results indicated that the mutation of gene RYR1 do not represent an influential disorder in breeds harbored in Czech Republic. Sporadic presence of this mutation was consulted with commercial genetic laboratories.
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The study of the role of biotransformational enzymes in chemical carciogenesis
Kondrová, Eliška ; Souček, Pavel (advisor) ; Skálová, Lenka (referee) ; Vobořilová, Jana (referee) ; Bořek Dohalská, Lucie (referee)
In vitro biotransformation studies are an integral part of both toxicological research and drug development. They allow for a significant reduction of tests on human volunteers and provide detailed information about the metabolism of a given compound. Due to limited availability of human liver tissue, it is necessary to make use of alternative model systems and model animal species in the study of the interactions between biotransformation enzymes and xenobiotics. Since the activities of the most important human biotransformation enzymes are absent in the most commonly used laboratory species, rat, information gained in experiments with minipigs is very valuable. Recombinant human biotransformation enzymes expressed in bacteria can be used in biotransformation studies in the form of isolated bacterial membranes. These model systems provide information about the metabolism of a given xenobiotic by a defined enzyme and thein advantages compared to purified enzymes include low cost and quicker and easier preparation with lower loss of enzymatic activity during isolation. The aims of this study were: 1) To prepare a model system with recombinant human biotransformation enzymes expressed in bacterial membranes and to compare the properties of this system with minipig liver microsomes. To assess the usefulness of...
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Changes of diversity and abundance of aquatic avifauna in relation to the physical-geographical environment
Adam, Matyáš ; Romportl, Dušan (advisor) ; Chuman, Tomáš (referee)
Changes of diversity and abundance of aquatic avifauna in relation to the physical-geographical environment ABSTRACT. This thesis looked into the issue of impact of changes of suitable habitat structure and climatic conditions, as the most discussed environmental factors, on model group of organisms - waterbirds. In the first part there was characterized effect of these factors on model group by the available literature. The relevant environmental variables were processed in the second part. For analysis of impact of these environmental factors on abundance and diversity of aquatic avifauna there were used data from International Waterbird Census in January term from 93 sites throughout the Czech Republic. Data were chosen for the period 1975, 1990 and 2006, in accordance with the database CORINE Land Cover. As multivariate data analysis there were used redundancy analysis (RDA) and mixed models with random effects, which showed significant associations with both weather conditions and other environmental factors. Selected environmental variables contributed substantially to the explanation of variability in species data, that was also tested suitability of their use. Keywords: physical-geographical environment, climate change, land cover, habitat structure, biodivesity, waterbirds
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Traditional Methods of Fish Preservation in Southeast Asia
Holmanová, Kateřina ; Banout, Jan (advisor) ; Leuner, Olga (referee)
Traditional methods of fish preservation are still often used in developing countries, which produce significant amount of fresh fish. The most frequently used methods of processing fish in Cambodia are drying, salting, fermentation, smoking and marinating. Sun drying as one of the most common methods of fish processing in Cambodia has some disadvantages, which increase spoilage of final product. Salting contributes to increase efficiency of drying process. Salt creates highly salty environment, which the most bacteria, fungi and other potentially pathogenic organism cannot survive in. The aim of this Bachelors thesis was to carry out research focused on drying method in combination with salting. For five selected spices of freshwater fish: Claris batrachus, Channa micropeltes, Oreochromis niloticus, Pangasius hypophthalmus and Monopterus albus were determined dry matter content, fat content and salting effect on the amount of salt. Samples, before drying in direct solar dryer with natural convection and electric oven, were salted for 2, 4 and 12 hours. Results of measuring of dry matter content are within the interval 16.67 to 21.74 g on 100 g of fresh muscle, the lowest values were measured at C. batrachus and the highest at O. niloticus. Measuring of fat content showed difference in the interval 2.29 - 30.08 %, the lowest value at M. albus and highest at P. hypophthalmus. From the measurements of amount of salt were found influence of the salting time on the total amount of salt. Values increased with the higher time of salting. An important prerequisite for achieving high-quality dried products is characteristic properties for individual fish species to achieve maximum yield.
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Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.)
Slowiaczek, Lukáš ; Hnilička, František (advisor) ; Jan, Jan (referee)
Diploma thesis with the topic: Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.) was worked out at the Department of botanics and plant physiology, Faculty of agrobiology, food and natural resources ČZU in Prague. The purpose of this thesis was to determine genotype and ontogenetic differences in amount of yield, production of dry mass, and value of growth-analytic characteristics in certain species of opium poppy. Diploma thesis was based on the fact that most of the studies about opium poppy (Papaver somniferum L.) are focused on the content of alkaloids and other substances present in poppy seeds. Due to this facts, it is necessary to describe the genotypes of poppy plants and their genetic resources from the physiological point of view.
Following species of opium poppy were chosen for the experiment: Akvarel, Albín, Buddha, Florian, Korneuburger Weisser, Lazur, Major, Marianne, Opál, Postomi, Sokol, Tatranský. These species were chosen according to their content of alkaloids, especially morfine very low content, low content, medium content and high content of morfine. All of the tested genotypes are tested as object of study of genetic poppy resources. Seeds were supplied by GB Oseva Pro s.r.o., o.z. VÚO Opava and company Český mák.
Field experiments were established at the research laboratory FAPPZ ČZU in Prague, in Červený Újezd. This location is in Prague-West division, in area of Middle Bohemia. The altitude of this location is 401 metres above the sea level. The size of the field for testing was 2 m2. The experiment was based in 4 repetitions by the method of Latin square. Winter wheat was used as the preceding crop. Opium poppy was planted according to agrotechnical principles of planting.
Content of dry mass in individual plant organs was measured in certain ontogenetic stages of opium poppy (35, 45, 49, 52, 54, 62, 81 BBCH). Size of leaf area was assessed by the image analysis WinDias. Yield of seeds was assessed as well. Growth-analytic characteristics were calculated from the values of wieght of dry mass and size of leaf area.
The results confirmed that the size of plants is influenced by its ontogeny and also by its species. The results also confirm, that the height of plants is not influenced by the content of morfine. Connection between the colour of seeds and height of plant was not confirmed either. Increase in weight of above-ground biomass depending on ontogenetic stage and influence of plant species was confirmed. There are differences in production of yield in species of poppy with different contents of morfine because the average weight of yield in species with very low content of morfine was 112,36 g, in species with low content of morfine 110,41 g, in species with middle content of morfine 12167 g and in species with high content of morfine 123,16 g. It was approved that ontogeny of plants influences the leaf area index (LAI) as it increases until blooming and then it decreases as a result of senescence and falling off the old leaves. Differences in leaf area index were detected among the species of opium poppy, depending on the content of morfine. It was detected that crop growth rate (CGR), relative crop growth rate (Rw) and relative growth rate of assimilation area (RA) are influenced by the ontogeny of plants. It was detected that species with high content of morfine in poppy seeds (Buddha, Postomi, Lazur) and species with white seeds Sokol and Albín have lower yield than species with middle content of morfine.
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Genetic Diversity of Semi-captive Population of Western Derby Eland (Taurotragus derbianus derbianus) in Senegal and Phylogenetical Relationships between Western Derby Eland (T. d. derbianus) and Eastern Giant Eland (T. d. gigas)
Zemanová, Hana ; Lukešová, Daniela (advisor) ; Jarmila, Jarmila (referee)
Representatives of family Bovidae are subjects of many studies concerning with their phylogeny, phylogeography, time of divergence or genetic diversity. Taxonomy is solved by comparison of morphological characteristics or by genetic approaches, genetic diversity could be solved by pedigree or by genetic analyses too. Tragelaphinae number nine species of two genera, Tragelaphus sp. and Taurotragus sp. The antelopes of the genus Taurotragus (T. derbianus and T. oryx) belong to the largest antelopes of the world. Derby eland (Taurotragus derbianus) has two subspecies, Western Derby eland (T. d. derbianus) and Eastern Giant eland (T. d. gigas), which are distinguished on the basis of morphological characteristics. Western subspecies (T. d. derbianus) is classified as critically endangered. There lives the only population in Niokolo Koba National Park in Senegal, which numbers fewer than 200 individuals. For the conservation, the semi-captive breeding programme has been established in 2000. It was created by six founders (one male and five females), which are presumed to be non-related. The population within this programme had 95 living individuals in 2013, living in seven herds in Bandia and Fathala reserves in Senegal. The population is under breeding management, which efforts to minimize kinship of the individuals. Studbook was established for the Western Derby eland (T. d. derbianus) in 2008 and is published annually. It acts about small population with low number of founders and no gene flow, which is threatened by inbreeding and genetic drift. Genetic diversity of the population was evaluated by means of microsatellite markers and the results were compared with the results of pedigree analysis. Pedigree analysis showed the highest genetic diversity in the generation of founders (FOUNDERS). It decreased in the generation of founders' offspring (OFFSPRING 1; born in season 2007/2008), due to the fact, that the only male took part in the reproduction. And it increased again in the generation of offspring of founders' offspring (OFFSPRING 2; born in the season 2009/2010), because more individuals were included into the reproduction. Fifteen individuals and five polymorphic microsatellite loci (from the total number of 13 tested loci) were chosen for the genetic study. The parameters of genetic diversity (HE and HO, Ar and deviations from Hardy-Weinberg equilibrium, and FIS and FST) were evaluated. Not any deviations from Hardy-Weinberg equilibrium were found out. The results of genetic analysis confirmed the highest genetic diversity in the population of founders (Ar = 2.79; HE = 0.664; HO = 0.750; FIS = --0.154). In both generations of offspring values of allelic richness and observed and expected heterozygosity decreased (Ar = 2.15; HO = 0.580; HE = 0.586 in OFFSPRING 1 and Ar = 2.14; HO = 0.370; HE = 0.480 in OFFSPRING 2). Contrary to the results of pedigree analysis, there was not been observed any improvement in OFFSPRING 2. The resultant values of genetic diversity parameters were quite satisfactory, despite of the low number of founders and mating of related individuals.
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