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A comparison of unemployment in various social groups
Urbanová, Věra ; Majerová, Věra (advisor) ; Petra, Petra (referee)
This diploma thesis has both a theoretical and a research section. In the theoretical part basic concepts related to the topic are defined and the unemployment of social groups vulnerable in the labour market that require increased attention in their inclusion are analysed. The empiric section comprises of research focusing on candidates of these social groups applying for a job. Furthermore, the research of the labour office's employees at the departments of the labour office (KoP ÚP ČR) is included. The acquired data is then evaluated and a conclusion formed as to how the current situation can be improved.
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Attitudes of Secondary School Students towards Sport and Other Physical Activities
Bílá, Olga ; Jansa, Petr (advisor) ; Chalupová, Eva (referee)
Název: Názory a postoje studentů středních škol na sport a jiné pohybové aktivity. Attitudes and opinions students of secondary school to sport and other locomotive activities. Cíle práce: Záměrem této práce bylo zjistit názory a postoje mládeže na význam sportu v jejich životě, účast na sportovních, tělovýchovných a jiných pohybových činnostech. Dále měl výzkum přinést informace o oblibě různých pohybových aktivit a také nejvýznamnějších motivů pro sportování a s tím souvisejících nejdůležitějších bariér, bránících aktivnímu sportování. Celkový výzkumný úmysl byl zaměřen na srovnání názorů a postojů mládeže gymnázií a učilišť. Metody: Pro výzkumné šetření byl využit nestandardizovaný dotazník. Celkově bylo v dotazníku 38 otázek, rozdělených do několika témat. Výzkum probíhal na školách v Pardubickém kraji v průběhu září a října 2005. Celkově bylo dotázáno 634 osob. Výsledky: Naším úkolem bylo srovnat a prokázat názorovou a postojovou orientaci mezi typem střední školy (gymnázia, učiliště) a mezi pohlavím (muži, ženy). Tyto výsledky ve většině případů potvrdily naše pracovní hypotézy. Celkově můžeme hodnotit názory a postoje mládeže 15-18leté ke sportu, tělovýchově a jiným pohybovým aktivitám za příznivé a pozitivní. Klíčová slova: adolescentní mládež, pohybová aktivita, sport, názory a zájmy,...
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Biotransfer of selected risk metals into plants and their accumulation and distribution in plant organs
Le Minh, Phuong ; Lachman, Jaromír (advisor)
Contamination of soils with heavy metals is one of the serious environmental problems threatening human being. Heavy metals are considered as the special hazard of soil pollutants because of the adverse effects on the plant growth, the amount, activity of useful microorganisms in soils and the quality of food. Regard to the persistent and toxicity, the heavy metals are toxic when we consider different kinds of pollutants in soils. In the soil, zinc (Zn), cadmium (Cd), lead (Pb) and mercury (Hg) toxicities frequently occur than the other metals because of their precipitation and sorption by the soil. It is a very dangerous situation because when these metals are taken up by plants, they can be transported to the food web and food chains.
In the present study, the accumulation of four heavy metals (mercury, zinc, lead and cadmium) in the whole grain of spring accessions of emmer, einkorn and common spring wheat cultivars and potato (Solanum tuberosum) is reported. Heavy and essential elements were monitored in potato cultivars in the exact field experiments and in hydroponically grown plants. The elements were determined by methods FAAS, ET AAS, and AMA (Advance Mercury Analysis). Statistical analyses were performed using SPSS 9.0 with the Tukey HSD (Honestly Significant Difference) test (alpha equal to 0.05).
In our study, the concentration of heavy metals decreased in the order zinc (Zn) > lead (Pb) > cadmium (Cd) > mercury (Hg) in the wheat grain. The comparison between three varieties of investigated wheat revealed that the emmer wheat was rich in zinc content (62.12 mg kg-1 dry matter), while the spring wheat had the lowest average concentration of zinc in the grain (40.99 mg kg-1 dry matter). Generally, the values of lead concentration in grain wheat varieties were low (ranging from 0.1268 mg kg-1 dry matter to 0.2950 mg kg-1 dry matter).
The concentrations of mercury in four typical growth stages of wheat (boot stage 10, heading stage 10.2 1/4 of head emerged, leaf-stage 10.2 and stage ripening 11 according to Feekes) were also determined. It has been shown that the concentrations of mercury in different wheat varieties were absorbed differently at different growth stages of plant. Stage 10.2 and leaf stage 10.2 showed the high mercury content (0.0152 mg kg-1 dry matter and 0.0214 mg kg-1 dry matter, respectively). Among individual varieties significant differences were determined.
Amounts of toxic and potentially toxic elements detected in investigated potato tubers are characterized by a large variability within investigated groups. Performing statistical analysis (one way ANOVA) showed that there were no significant differences between two investigated groups of samples (samples from Uhříněves and Valečov in the year 2013 and 2014) considering either one of investigated metals. Measurable levels of mercury were found in smallest amounts in all investigated potato samples comparing to other metals (Cd, Pb).
Plant cells compared to animal cells are characterized by the formation of cell walls. Plasma membrane or cell membrane is a biological active membrane separating the interior of cell from the outside environment. An adjusted method for isolation of protoplasts was developed and adapted for isolation of protoplasts from plant material (potatoes). In our experiment, the plants were grown hydroponically in the Research Institute of Plant Crops Prague-Ruzyně. If we examine the plant membrane, one option is to remove the cell wall by means of special mixture enzymes. Protoplasts were released in the dark at 25 degrees of Celsius for 18 hours. The 70 and 90 microns sieve was used to filter and then centrifugation for 5 minutes at 100 x g. All the steps were carefully carried out to prevent the damage or breakage of protoplasts.
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Mophological and molecular diversity of a tropical tree species Guazuma crinita in the Peruvian Amazon
Tuisima Coral, Lady Laura ; Lojka, Bohdan (advisor) ; Helena, Helena (referee)
Fast growing tree species Guazuma crinita (Malvaceae) was selected as a priority species for domestication in the Peruvian Amazon due its important contribution to the livelihood of local farmers. Its domestication process is still in an early age as for many tropical tree species little is known about its genetic variability and we dont know anything about the impact of domestication on its genetic resources. The main objective of this research was to assess the genetic variability of G. crinita within and among populations in the Peruvian Amazon by the use of morphological (wood physical traits) and molecular (ISSR and AFLP) markers.
Wood physical properties among six G. crinita provenances were evaluated. Wood samples were drilled from the base middle and top of the stem of 12 randomly selected eight-years-old trees for determination of wood measurement. Pearson correlations between physical properties were also determined. All wood physical properties except green density differed significantly among provenances. We also found statistically significant variation due to stem level position. The moderately dense wood and the coefficient of anisotropy (1.6) suggested that G. crinita has stable wood; they represent important advantages in terms of costs for transport and transformation process. The results suggested potential to select provenances with desirable wood properties for further breeding and domestication. Due to the variation found even in limited tree samples it is recommended further analysis with more extensive number of samples from different provenances and planting zones.
This research presents the first assessment of genetic variability based on inter-simple sequence repeat (ISSR) markers for 44 G. crinita genotypes from a clonal garden multiplication established in the Peruvian Amazon Research Institute (IIAP) in Ucayali region. Ten ISSR primers amplified a total of 65 bands of which 61 were polymorphic (93.8%). The range of DNA amplification varied from 260 to 2200 bp. Among the provenances overall genetic differentiation (Gst) was 0.03 indicating 97% of genetic diversity within provenances. Gene flow (Nm) was 12.9 alleles per generation. Cluster analysis was not related with geographic origin suggesting a common gene pool which was supported by calculation of weak positive correlation was found between genetic and geographic distance.
With the use of AFLP markers an insight on how domestication process does impact G. crinita genetic resources is also reported on this research work. I was able to generate fingerprint for 58 leaf samples representing eight provenances and three population types, 19 from a natural regenerated population 15 cultivated in home garden nursery and 24 from a collection of genotypes considered as semi-domesticated population. Seven selective AFLP primer combinations were used. A total of 171 fragments were amplified with 99.42% of polymorphism at species level. Each type of population generated fragments with 72.51% 49.12% and 54.39% of polymorphic fragments respectively. Neis genetic diversity and Shannon index information were found to be higher in the population of natural regeneration compared to overall semi-domesticated population (He = 0.10 and 0.9; I = 0.19 and 0.16 respectively). The analysis of molecular variation (AMOVA) showed higher variation within provenances rather than among (84% and 4% respectively). UPGMA clusters analysis and PCoA did not showed correspondence between genetic and geographic distance in addition their correlation was not significant. There was a significant genetic differentiation among types of population suggesting slight genetic bottleneck in semi-domesticated populations yet with relatively high levels of genetic variation.
In situ conservation for populations with high levels of genetic diversity was recommended. In addition proper management of natural regeneration and ex situ genotype collections might be a good conservation strategy to maintain G. crinita genetic resources.
The use of morphological (wood physical traits) and molecular markers were successful to reveal genetic variability of G. crinita and they could be used for other tropical tree species. For further researches it is emphasis to extent the number of samples and geographic scale.
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Diagnotics of the important apple viruses
Winkowska, Lucie ; Ryšánek, Pavel (advisor) ; Petr, Petr (referee)
Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple chlorotic leaf spot virus (ACLSV) are economically the most important viruses in pome fruit trees, which are distributed worldwide and can caused significantly yield reduction. The major control strategies (namely pathogen detection, exclusion by crop certification or quarantine, control in infected orchards by eradication from infected cultivars and rootstocks, etc.) rely heavily on accurate and sensitive detection methods and on perfect knowledge of pathogens.
In the doctoral thesis the diagnostic method quantitative RT-PCR (qRT-PCR) was optimized for detection and quantification of four studied viruses. The results suggested that qRT-PCR method was the most reliable technique in comparison with conventional diagnostic methods DAS/I-ELISA and RT-PCR.
In our study the concentration of ASGV, ASPV and ACLSV, measured by qRT-PCR, were stable during vegetation and in different plant tissue. Only the concentration of ApMV changed during vegetation in leaves and inner bark. This result indicates that changes of virus concentration observed by DAS/I-ELISA and RT-PCR in plant tissues are caused by other way (inhibitors, plant senescence, lower sensitivity, ect.) than by changes of virus concentrations in plant.
Under the monitoring (at all 351 trees were tested) it was showed, that studied viruses were more spread in orchards and gardens then in wild apple trees.
Selected virus isolates from wild apple trees and apples from orchards and gardens were sequenced and molecular variability was studied also with already published isolates. However individual isolates of studied viruses were similar. The variability associated with geographic origin or with type of planting has not been confirmed.
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Behavioural selection criteria for Czech police puppies to assess their chances of certification.
Hradecká, Lenka ; Bartoš, Luděk (advisor) ; Martin, Martin (referee)
The project based on behavioural tests of 7 week old German Shepherd puppies performed in police kennel in Prackovice nad Labem and Domažlice. The project focused on verification of the usability and improvement of the selection of puppies using behavioural test. We tested the possibility of environmental effect (presence of shooting range near the kennel Domažlice) on the later success of puppies in the behavioural test. The aim of the project was collecting additional data which were use to improve and verify procedure for early selection of puppies. We checked the possibility of habituation breeding females and their puppies to noise, including monitoring of the different environments in the kennels in. It was found that different results are affected by of their mothers. This means that puppies raised by mothers from the police kennel near shooting range are better evaluated later in the disciplines of evaluating response to noise compared with pups from mothers of police kennel without shooting range. Worse response to noise had pups from litters with a higher number of pups.
Reviews summarizing the literature on heritability of behavioural traits in dogs have been published repeatedly over the last four decades. The conclusions of these reviews vary to some extent. We applied meta-analysis and re-evaluated the literature available collecting 47 references dealing with heritability of behavioural traits in domestic dogs. For each study included, data was extracted for individual behavioural traits. Each of these traits was entered into the analysis as a separate unit of measure, giving a total of 1761 measures. We classified the traits measured in each study into five functional categories (Environment, Herding, Hunting, Play, and Psychical characteristic). The metaanalysis has shown low general heritability of the categorised behavioural traits analysed. Thus, the commonly used behaviour tests for many breeds, to determine the dog's eligibility for reproducing, traditionally required by many associations, is not supported by the present results of the meta-analysis.
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Application of mathematical models for simulation of hydrological conditions in selected streams
Kurková, Marie ; Vašků, Zdeněk (advisor) ; Michal, Michal (referee)
Flood is a natural phenomenon that occurs at different intensities and irregular time intervals. As to natural disasters, floods represent the greatest direct threat for the Czech Republic. They may cause serious critical situations during which not only extensive material damages are done, but may bring also losses of the lives of inhabitants in affected areas as well as vast devastation of cultural landscape including environmental damages. Important from the viewpoint of the elimination of potential threats and consequences of such events is the information issued by flood forecasting service about the character and size of flood areas for individual N-year flood discharges and specific flood scenarios. An adequate image of depths and flow rates in the longitudinal or cross profile of the watercourse during a flood event is provided by the hydrodynamic model. This is why the information obtained from the hydrodynamic models occupies a privileged position from the viewpoint of the protection of citizens' lives and mitigation of damage to their property. The first study is situated on the river Úhlava in meadows by Příchovice near the town Přeštice. The proposal of flood-protection measures is contained in Territorial control documentation. The documentation was elaborated on the basis of hydraulic calculations and experiences from the flood in August 2002. The mathematical model is practically used in the study of analysis of proposed flood-protection measures. The analysis is based on mathematical simulation of water outflow and water level on the river Úhlava. It is possible to use the non-commercial software Hec-Ras, version 3.1.1., for the simulation itself. One of the points of view of the possibility of using proposed flood-protection measures is total efficiency. The mathematical model is posssible to use as a basis of support for realization of proposed flood-protection measures on the river Úhlava in meadows by Příchovice within the grant programme "Program prevence před povodněmi II" under the control of the Ministry of Agriculture. In the second case the mathematical model is practically used in the study of hydrotechnical analysis of streams in cadastral unit. The analysis is based on matjematical simulation of water outflow and water level on chosen streams. It is possible to use the noncomercial software HEC-RAS for the own simulation. The analysis should be shown on dangerous places in the interest place. The mathematical model is possible of using to use as basis for revaluation of action in spatial plan or for view of the flood-protection measures in the village Mochtín. Basic input into the hydrodynamic models is represented by altimetry data. One of ways to obtain such data is through the method of aerial laser scanning (ALS) from the digital relief model (DRM). This method is considered one of the most accurate methods for obtaining altimetry data. Its bottleneck is however incapacity of recording terrain geometry under water surface due to the fact that laser beam is absorbed by water mass. The absence of geometric data on watercourse discharge area may perceptibly affect results of modelling, especially if a missing part of the channel represents a significant discharge area with its capacity. One of methods for eliminating the deficiency is a sufficient channel recess by means of software tools such as CroSolver. The third submitted paper deals with the construction of a hydrodynamic model using 5th generation DRM data, and compares outputs from this model at various discharges with a model based on the altimetry data modified by using the CroSolver tool. Outputs from the two hydrodynamic models are compared in HEC-RAS programme with the use of recessed data and with the use of unmodified DRM. The comparison is done on the sections of two watercourses with different terrain morphology and watercourse size. A complementary output is the comparison of inundation areas issuing from both model variants. Our results indicate that differences in the outputs are significant namely in the lower discharges (Q1, Q5) whereas for Q50 and Q100 the difference is negligible with a great role being played by morphology of the modelled area and by the watercourse size.
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Analysis of coffee sales in selected hotels in Prague
Veverka, Libor ; Kopecká, Lenka (advisor) ; Svoboda, Roman (referee)
In this thesis the sale of coffee in selected hotels in Prague will be analysed, based on information obtained in the drink menus of these hotels.
The theoretical part consists of three main parts, namely The market and the market mechanism, Classification by type of prepared coffee drinks and The classification of hotels into five classes.
The first part, called The market and the market mechanism, explains market and its functioning, perfect and imperfect competition between companies and, in the end also imperfections of the market. The second part of Classification by types of prepared coffee drinks discusses the ingredients and the proper preparation of a wide range of coffee drinks. The third and last part of the theoretical part called The classification of hotels into five classes, points out the basic limit points for dividing hotels into each of these categories.
In the second part, which is called The actual work representations of hotels in the Czech Republic, in Prague are examined and compared. There is also a list of the selected hotels in Prague, which are taken into account in the analysis, comparison and subsequent assessment of results.
In the conclusion, the difference in prices of offered coffee drinks in randomly selected hotels in Prague is assessed from two points of view. The first is examining the differences in prices between classes of hotels and the second one is examining differences in prices inside the particular classes.
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Motivation as a tool of performance management
Korbel, Petr ; Kříž, Josef (advisor) ; Hana, Hana (referee)
The thesis Motivation as a tool for performance management describes motivation of employees in company Strojmetal Aluminium Forging. It furhter analyzes human resources management in the Company and its impact on performance of its employees.
The aim of the study is to evaluate current motivation process within the Company and to propose new tools for improvement of performance management of its employees. The target group of this study are technical and economical employees of the Company.
The theoretical part of the study defines basic terms such as motivation, performance of employees, and tools for performance management, reflecting a variety of modern approaches within this area.
The practical part of the study focuses on current performance management of the Company and uses theoretical assumptions to evaluate the current performance management and to predict development of the performance management in a timeline of two years.
The underlying data for the study were gathered from internal sources and from controlled interview with the head of the pesonnel department of the Company.
Subsequently, using a questionnaire survey, performance of employees and tools for performance management of the Company were assessed and evaluated. The research was conducted at the end of year 2015.
Based on the evaluation of the results of the study, their comparison with the benchmarks and with the predicted motivational factors in a timeline of two years, recommendations for achieving greater effectiveness of the current motivation process and its sustainability were suggested.
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Fiscal and monetary policy in the Czech Republic
Petroušková, Barbora ; Toth, Daniel (advisor) ; Helena, Helena (referee)
This diploma thesis deals with fiscal and monetary policy and their effect on economic development. The work is composed of theoretical and practical part.
In the theoretical part are defined and described individual policies, including economic policy and their objectives, types, forms, tools and problems.
The practical part analyzes the development of the Czech economy between 2004 and 2014. Also is solved econometric equation for inflation. In equations are achieving macroeconomic indicators: the annual change in the average nominal monthly wage, rate of household savings, inflows foreign direct investment, unemployment, gross domestic product and net exports. The same equation is solved in the Slovak Republic, which was chosen as relevant for comparison of economic development, basic data are using from the same period. An econometric model is solved by ordinary least squares method. Based on the analysis of economic development in the Czech Republic and results of the econometric model of inflation in Czech and Slovak Republic is proposing a change in the fiscal and monetary policy for the Czech Republic.
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