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Influence of water deficit on physiological characteristics of selected species of green leafy vegetables
Kraus, Kamil ; Hnilička, František (advisor) ; Dana, Dana (referee)
The harmful influences on plants are called stress factors. The stress itself does not slow down only vital functions of plants but influences directly the size and quality of final product as well.
Worldwide, we count the so called abiotic stresses as the most significant crop production stressors. These include in recent years extremely high temperatures and unequal rainfall distribution both areal and during vegetation period.
The water deficit (drought) belongs to the most important stress factors in agriculture. It influences crop production all over the world. Also vegetables are responsive to water deficit, especially the species with huge amount of water in their tissue. Therefore, the goal of this thesis is to assess the impact of water stress on gas exchange and photosynthetic activity of mangold "Beta vulgaris", salad rocket "Eruca sativa" and lettuce "Lactuca sativa", which were placed at water deficit.
The plants - mangold, salad rocket and lettuce - were grown in a partly controlled greenhouse conditions under the Department of Botany and Plant Physiology FAPPZ ČZU in Prague. The plants were grown at a temperature of 25°C in daytime and 18°C at night under natural light conditions. They were grown in containers of size of 11x11 cm in a mixture of garden substrate A and silica sand in the ration 2:1. The experimental scheme included two variants - control and stressed. The control group of plants was watered, and the second half of plants was left under stress for 22 days, when the water deficit was induced by gradual drying for one month, the experiment took place from 1.6 2015 to 22.6 2015. The speed of gas exchange by plants was measured by apparatus LCpro+. From the values of photosynthesis and transpiration water use efficiency (WUE) and chlorophyll fluorescence were calculated.
The gained results proved difference at reaction to water stress between observed kinds of leaf vegetables.
The speed of photosynthesis and transpiration of observed plant species was measured, which decreased due to impact of water deficit. The average speed of photosynthesis of stressed plants was the lowest by lettuce (11,16 "mikro"mol CO2.m-2.s-1), contrarily the highest by mangold (13,2"mikro"mol CO2.m-2.s-1). In case of control plants was the lowest speed of photosynthesis noticed by lettuce (12,03"mikro"mol CO2.m-2.s-1) and the highest by mangold (14,00"mikro"mol CO2.m-2.s-1). In comparison with control group, the speed of photosynthesis of lettuce decreased significantly by impact of water deficit (difference make 7,23 %). On the other hand the lowest decrease of photosynthesis from stressed plants 5,93% was noticed for mangold.
In case of speed of transpiration, it is possible to note, that the lowest transpiration of control group shows mangold with the average speed of transpiration 1,82 mmol H2O.m-2.s-1, by contrast the highest transpiration was measured by lettuce 3,20 mmol H2O. m-2.s-1. In the case of stressed plants moved the average speed of transpiration between 1,54 mmol H2O.m-2.s-1 for lettuce and 1,79 (mmol H2O.m-2.s-1) for mangold. Also, between control and stressed plants of the same species were found differences, and salad rocket reacted to stress of drought more sensitively and mangold less sensitively. For these two plant species was noted decrease of transpiration in the amount of 23,80% and 1,76%.
From the measured value of photosynthesis and transpiration was calculated water use efficiency (WUE). The calculated values show that stressed plants have higher value of WUE in comparison to control plants. The lowest water efficiency from control plants produced salad rocket
(6.51 x 10-3), while the highest mangold (10.31 x 10-3). In the case of stressed plants was the lowest value of WUE noted for salad rocket (8.96 x 10-3), while for mangold was the highest (10.71 x 10-3).
Chlorophyll fluorescence of the experimental plants was also measured. This characteristic was not influenced by plant species, because in case of control plants, there were no differences found between observed plant species. The lowest difference in value of fluorescence had plants of lettuce (0,69) while the highest mangold and salad rocket (0,7). In conclusion it is possible to note, that more sensitive to water deficit are plants of lettuce. As more tolerant to water deficit seems mangold and salad rocket.
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Alternative components in feeding rations for marmosets and tamarins as prevention of macrosomia, diabetes mellitus and stillborn young
Polívková, Martina ; Svobodová, Ivona (advisor) ; Adéla, Adéla (referee)
This thesis evaluates the suitability of food rations in zoos keeping Callitrichidae considering the prevention of macrosomia, diabetes mellitus and stillborn young, and where needed, suggests a change in feed rations.
The feed rations of Callitrichidae in zoos differ greatly and problems can occur with greater amounts of fat and sugars and often also insufficiency of calcium, which can lead to obesity and further health complications.
When performing this thesis, all food rations of the pygmy marmoset (Cebuella pygmaea), the silvery marmoset (Mico argentatus), the cotton-top tamarin (Saguinus oedipus), the emperor tamarin (Saguinus imperator) and the golden lion tamarin (Leontopithecus rosalia) was weighted during five days in the Olomouc zoo, the Plzeň zoo and the Jihlava zoo. The values of the rations were set based on table rates of the individual components stated in the program ZootritionTM (V2.6) and the final values compared with the recommendations stated in EAZA Husbandry Guidelines 3rd edition (2015).
It was found that changes are needed especially with consideration to the food specialization of the different species, presently all species of Callitrichidae are fed the same rations regardless of their food priorities in the wild.
The recommended changes to rations included changing a part of the ration to a ready mixed foodstuff for Callitrichidae to save time and enable a stable ration with good values of calcium and protein, and feed the animals mainly with vegetables rather than fruit, considering the closeness of vegetables to the fruit available in the wild.
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Legislative requirements for milk quality in the Czech Republic
Škvorová, Kamila ; Hejtmánková, Alena (advisor) ; Michlová, Tereza (referee)
The aim of the thesis was to give a comprehensive review of literature dealing with legislative measures, milk production in terms of sales of milk and its storage. We used the most current Czech and European legislation. These sources have been supplemented thematically compliant scientific and professional publications.
Milk is a primary since the Middle Ages and sought food which people consume every day. Along with dairy milk a deserved popularity mainly due to its rich composition and nutritional and health properties. The main components of milk include water, lactose, fat, protein and minerals. Milk however can also include foreign substances of organic and inorganic origin and unwanted microflora. Microorganisms are particularly dangerous to humans and are considered a major source of foodborne illnesses. To eliminate unwanted microflora, extending shelf life and maintain public safety is used heat treatment, including pasteurization and treatment of very high temperature (UHT). This treatment must be carried out according to established requirements and specifications.
If a consumer buys a dairy product in the Czech Republic retains its quality as well. All animal products, including products imported to the Czech market, are compulsorily fitted with oval health mark, which ensures wholesomeness. The quality of the milk is influenced by many factors, most of which are closely linked, and therefore not always easy to identify the specific cause possible reduction in quality standards. Already during the production process, emphasis is put on prevention in the field of quality and safety. Security is guaranteed by a system of determining critical control points HACCP, which is in the Czech Republic and the EU compulsory by law. Among the optional systems include ISO. Most enterprises with good reputation has introduced both these systems. Simultaneous application of HACCP and ISO standards provides a guarantee for the security of the overall quality and safety of products. This connection is known as TQM system.
The main indicators of quality of raw milk are total number of mesophilic microorganisms, somatic cell count, fat content, crude protein residues of inhibiting substances, the average herd size and the number of bred cows. The milk must not contain any preservatives or stabilizers. Meeting the requirements and quality criteria will be checked on the appropriate number of samples that are collected in enterprises producing milk, random checks.
The basic quality criteria of raw milk, food business operators must ensure that:
The content of microorganisms in 1 mL at 30 ° C must be < 100 000. It is found average values for two months for the collection of at least two samples per month.
Somatic cells in 1 ml at 30 ° C must be < 400 000. It is obtained by moving the geometric mean for the three months, every month, evaluates at least one sample.
Immediately before treating the milk must contain 1 ml < 300000 microorganisms at 30 ° C. In the treated milk that is used for the manufacture of other dairy products, may be the content of microorganisms in 1 ml> 100,000 at 30 ° C.
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Effect of body condition on reproductive capabilities of Blonde d´Aquitaine cows
Kopečková, Tereza ; Stádník, Luděk (advisor) ; František, František (referee)
The purpose of this thesis was to determine the influence and mutual relation between the body condition of cow and the weight of calfs at birth, at 120 and 210 days of age. Next, the influence of the breeding cows body condition on the conception rate was examined. As hypothesis served the assumption that cows with optimal body condition (assesed via the BCS systém, levels 5 and 6) would have the highest conception rate and that their calfs would achieve the expected level of results at the inspection weighting. By contrast, at cows classified by the BCS system as a lower levels e.g. 4 or higher levels e.g. 7 and higher, the conception rate would decrease and the calfs would achieve worse levels at the inspection weighting.
For the evaluation, 81 cows with their calves were observed. The beginning of evaluation of the mothers condition began always approximately a week after the calving and further evaluations continued in monthly intervals. In total, six body condition assessments were made with every specimen. For the processing of data, the SAS 9.3 programme was used, namely MEANS, UNIVARIATE, CORR, REG and MIXED procedures.
The influence of BCS levels on the weight of calves at birth, at 120 and 210 days of age was not statistically significant (P > 0,05). But if we compare the occuring BCS levels in this work with the stated required range (BCS 5 to 7), the conclusion can be made that the results confirm the hypothesis. Also, a positive correlation occured between the occuring BCS level in the second assesment (P < 0,05) and the weight of calves at 120 days of age, as well as in levels of BCS in first (P < 0,01), second (P < 0,001), third (P < 0,05) assesment and the weight of the calves at 210 days of age.
The influence of individual BCS levels on the conception rate was also studied. Here, the influence was also not statistically confirmed (P > 0,05), but the comparison between the occurring BCS levels and the stated optimal range (BCS 5 to 7), the conclusion that the results confirm the hypothesis can be stated.
The order of cows calving has statistically important influence on the weight of the calves at birth, at 120 and 210 days of age. The best results were achieved by dams on the 6th and subsequent calving (P < 0,01 and P < 0,05). A positive correlation on the level of importance P = 0,05 was detected, between the order of the calving and pregnancy, but the statistically important influence was not confirmed (P > 0,05).
The influence of the calving month on the weight of the calves was confirmed only at weights at 210 days of age. The highest values were achieved by calves born in April (P < 0,01).
The influence of sex on the weight of the calves at birth, at 120 and 210 days of age was also studied. Higher values were achieved by bulls (P < 0,05).
The weight of the calves at birth affects the calving difficulty. Mothers with calves of lesser weight had demonstrably easier calving (P < 0,01). In the case of difficult calvings, the influence of calves weight on calving was not proven (P > 0,05).
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Evaluation of Screening Mammograms by Local Structural Mixture Models
Grim, Jiří ; Lee, G. L.
We consider the recently proposed evaluation of screening mammograms by local statistical models. The model is defined as a joint probability density of inside grey levels of a suitably chosen search window. We approximate the model density by a mixture of Gaussian densities. Having estimated the mixture parameters we calculate at all window positions the corresponding log-likelihood values which can be displayed as grey levels at the respective window centers. The resulting log-likelihood image closely correlates with the original mammogram and emphasizes the structural details. In this paper we try to enhance the log-likelihood images by using structural mixture model capable of suppressing the influence of noisy variables.
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Using of unseparated digestate as a phosphorus source for garden plants
Kujanová, Denisa ; Kulhánek, Martin (advisor) ; Jindřich, Jindřich (referee)
The advent of the modern trend of renewable resources was inevitable culmination of the development of civilization. Mankind learns more about use inexhaustible resources compared to depletion of reserves of non-renewable resources. Providers of renewable energy sources also include a biogas plant, which main function is the production of biogas. Biogas is produced in reactors by process of anaerobic fermentation without oxygen. As input for an agricultural biogas plants organic waste, cattle manure or for example energy grasses are used. The process of biogas production has the leftover of the fermentation at its end, so called digestate. Its composition depends especially on the inputs.
Digestate is a compound with high pH which is very rich not only for phosphorus but also for other nutrients. There are many studies dealing with the theory and practice of using digestate on the field for improving its properties and of other digestate usage. This diploma thesis has aim to explore combinations of unseparated digestate with peat and dolomite limestone with an effort to create a suitable substrate for different types of plants in terms of accessible phosphorus content.
During the experiment following plants were used between the years 2011 and 2015: Gazania rigens, Ocimum basilicum and Mentha piperita. For a control substrates following variants were used: Different types of commonly used growing substrates as the controls; peat combined with unseparated digestate in proportions of 5, 10, 15, 20, 25 % volume. In selected years dolomite limestone was also added in doses of 3, 6, 10, 15 g/l to stabilize pH.
The results of the work show a positive effect of the digestate on the substrate properties as for example pH value (with possible addition of limestone for stabilization) or dry matter. Digestate appears to be a suitable source of phosphorus for plants. In terms of the above-ground biomass yield of plants reached its maximum value at the control substrate Gramoflor. From the tested substrates it was in the most cases the substrate with 10 % digestate (in two years with doses of 6 a 15 g/l). Highest percentage of dry matter reached usually the control substrates.
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Public Opinion on European Union - April 2012
Tabery, Paulína
In April 2012 Public Opinion Research Centre investigated if Czechs believe, that decisions of the EU are in behalf of the Czech Republic and ordinary people (like themselves). There was also investigated, whether, according to Czech citizens, values such as democracy, equality, cooperation, tolerance, justice and solidarity are applied in the European Union. Moreover, CVVM probed satisfaction with EU membership and Czechs also talked about measure of their pride in context of being citizens of European Union.
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In vitro anti-proliferation activity alkaloids the Amaryllidaceae
Panenková, Kristýna ; Havlík, Jaroslav (advisor) ; Jarošová, Veronika (referee)
Summary
Natural phytochemicals are currently used in the treatment of many diseases. Cancers are just ones of them and they are ranked among the most common and the most serious. Phytochemicals in the form of cytostatics are used in chemotherapeutic treatment of cancer. In future there could be included among cytostatics also some alkaloids from the family
of Amaryllidaceae, whose testing for a selective cytostatic effect on tumor cell lines
of colorectal carcinoma Caco-2 and HT-29 and on normal cell lines of human intestinal epithelial FHs 74 Int is a subject of this thesis.
There were tested 17 alkaloids isolated from plants of Chlidanthusfragrans, Zephyranthes robusta and Nerine bowdenii. Particularly alkaloids from plant Zephyranthes robusta namely haemanthamine with this values: IC50 = 0.99 plus/minus 0.14 microM for tumor cells, Caco-2, 0.59 plus/minus 0.01 microM for tumor cells HT-29 and 19.47 plus/minus 8.86 microM for normal cells
FHs 74 Int, Lycorine with values IC50 = 0.99 plus/minus 0.08 microM for tumor cells Caco-2, 1.2 plus/minus 0.01 microM for tumor cells HT-29 and 22.68 plus/minus 0.09 microM for normal cells FHs 74 Int and Haemanthidin with values IC50 = 3.29 plus/minus 0.91 microM to tumor cells Caco-2, 1.72 plus/minus 0.11 microM to tumor cells HT-29, and 11.63 plus/minus 0.86 microM for normal cells FHs 74 Int proved a significant
anti-proliferative activity.
From these results there is evident the selectivity against colorectal cancer cell lines. For this reason, those tested alkaloids are suitable for further testing and for study of their biological activity against tumor cells in the terms of in vitro and in vivo.
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Process management in hotel facilities
POLÁKOVÁ, Lucie
The thesis is focused on the description of the company, its organizational structure and finance companies. There are also described each of the posts leading employees, their competencies and powers. The paper also analyzed the various processes taking place in the hotel facilities and there is value out of these processes.
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