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Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy
KOČOVÁ, Pavlína
This study is focused on changes in protein expression in a glioblastoma cell line during infection with tick-borne encephalitis virus. Newly synthesized proteins were distinguished from previously synthesized proteins using bioorthogonal chemistry (BONCAT method) to observe changes in protein synthesis. Labelled proteins were visualized using two-dimensional PAGE and western blotting followed by Click reaction on membrane. Differences in protein pattern between control and infected cells were observed.
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Tick-borne encephalitis risk assessment based on classification of vegetation from remote sensing data
Červená, Lucie ; Potůčková, Markéta (advisor) ; Pavelka, Karel (referee)
Tick-borne encephalitis risk assessment based on classification of vegetation from remote sensing data Abstract The main aim of this thesis has been to find out how to classify various categories of forest vegetation with a different risk of exposure to the tick-borne encephalitis based on the Landsat imagery. The legend used here is derived from the one used in the projects by Daniel, Kolář, Zeman (1995) and Daniel, Kolář, Beneš (1999) but has been reduced to only five classses with no overlaps in their definitions (I. coniferous stands, II. mixed stands, III. young deciduous stands and stand ecotones with a highly heterogeneous structure, IV. deciduous stands with a homogeneous structure, V. deciduous stands with a heterogeneous structure). The supervised classification with the Maximum Likelihood Classifier has been used on the Landsat imagery from various seasons. Difficulties concerned with the presence of clouds and varying Sun elevation across the imagery had to be adressed in the course of the work. The training sites and the control points have been defined by the field research and interpretation of the relevant orthophotomaps and Landsat imagery in 5-4-3 RGB composite. The mask of the forest has been created on the ZABAGED data basis. The time horizon of 2006 - 2010 has been the primary focus....
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Antiviral vaccination - tick borne encephalitis virus
Solarová, Pavlína ; Janštová, Vanda (referee) ; Drda Morávková, Alena (advisor)
Tick borne encephalitis is by one of the most severe disease of central nervous system. Agent of this disease is tick-borne encephalitis virus, which is transmitted mainly by tick. Clinical picture of TBE is considerably different and varied in individuals - from quite infection without symptoms up to severe cerebro-spinal meningitis, in such cases attacked persons by this virus leave hospital with whole-life handicap. We can prevent this disease by inoculation. We can see it in the neighboring Austria, where TBE almost became extinct because 90% of population is vaccinated. Vaccines are commercially available from 1979. Nevertheless only 17% population is vaccinated against TBEV. The reason for it could be fact that insurance companies do not provide the vaccines as well as not very effective health education. One of possibilities how we can this tendency reverse would be higher awareness of pupils at schools. In school educational programs there is contained prevention of viral disease, especially in general biology, biology of viruses and education to health.
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Tick-borne encephalitis - from pathogenesis to therapy
PALUS, Martin
The proposed thesis contributes to the knowledge about tick-borne encephalitis and its pathogenesis. The thesis describes pathogenesis and immunopathogenesis of tick-borne encephalitis, impact of host's genotype in clinical course determination, immune response of patients with acute tick-borne encephalitis, the mechanism of tick-borne encephalitis virus migration into central nervous system and virus interaction with cells of neurovascular unit as well as potential medical interventions.
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Tick-borne encephalitis - life with the consequences
ŠTIBINGEROVÁ, Jana
This thesis charts the course of the tick-borne encephalitis since the beginning to the eventual consequences that the illness had left and that the patient must live with. Ixodes ricinus, tiny parasites carrying the disease called encephalitis, is no longer present only in the wild, but also in city parks or in our gardens. Every year, many cases of this disease occur across groups of all ages. It is an inflammatory disease of the central nervous system caused by viruses. Disease is usually divided into two phases, where the first stage is similar to spring-summer influenza with different length of quiescent phase, in the second stage exacerbation occurs. At this stage, the patient is struggling with high temperatures, headaches, severe pain in muscles and joints, vomiting and disorders of consciousness. Correct diagnosis is specified after sampling of cerebrospinal fluid through a lumbar puncture. Patients with confirmed tick-borne encephalitis are hospitalized in isolation wards, not because of the spread of the disease, but for the experience of doctors. Here they are isolated in the rooms, which can have a detrimental effect on their psyche. Treatment follows after discharge from the hospital, and often takes several months. The aim of this study was to map the progression of the disease from its beginning, through time spent in a hospital bed, followed by home recovery up to the present. A very important part in the return-to-health process has the nurse, and therefore this paper tries to describe her role in treatment of patients with tick-borne encephalitis. Given that there exists a specific prevention in form of vaccination, the next objective was to verify the vaccination on samples of the population in the Česke Budějovice and Gmünd, Austria, and to compare these two groups of respondents. The survey of the thesis was carried out from August 2015 to April 2016 using a combination of qualitative and quantitative methods. Qualitative data were collected through semi-standardized interviews with nine respondents, who were recruited through social networks with the help of gatekeepers. Quantitative data was collected via short survey distributed in Česke Budějovice and Gmünd, Austria. The results of the qualitative part were processed through coding and compiling of diagrams, from which the answers of individual respondents can be deducted. Evident is the whole course of the disease from the beginning up to the present. Data from the quantitative section were arranged into tables and then statistically evaluated. Based on the results, the frequency of vaccination of specific groups can be compared and therefore campaigns on health protection against tick-borne encephalitis can be targeted effectively.
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Level of awareness and attitudes of parents to above-standard vaccination of children 0-3 years in Přibram.
PODLENOVÁ, Kateřina
This thesis is concerned with the level of parents´ awareness and attitudes to above-standard vaccination of children from 0-3 years in Příbram. Among the optional vaccination of children from 0-3 years belong vaccination against pneumococcal disease, rotavirus infections, meningococcal invasive disease (caused by meningococcal of group C, A+C, or A, C, W 135 and Y, now also of group B), tick-borne encephalitis, varicella smallpox, influenza and hepatitis A (or a combination of type A + B). The first part deals with the issue in a theoretical perspective. It foreshadows the basic characteristics of the diseases against which the premium vaccination of children from 0-3 years is offered. Further it is focused on vaccines against these diseases, which can occur in the Czech Republic. The survey is summarized in the research. There were parents of children attending one of the 12 kindergartens in Příbram in the sample of the research. tely 40% of the parents (or mothers) of these children in each kindergarten.The results were summarized in schedules of absolute and relative frequencies, or graphs. It was set three basic goals that were met with five hypotheses. The first of these was to monitor the attitudes of parents to above-standard vaccination of children from 0-3 years in Příbram. To this target relate hypothesis H1: Parents are interested in above-standard vaccination of children from 0-3 years in Příbram, H2: Parents with higher educational level have significantly higher interest in above-standard vaccination of children from 0-3 years and H3: Parents are statistically significantly more interested in vaccination against tick-borne encephalitis than other extra vaccination of children from 0-3 years, due to an endemic area of tick-borne encephalitis in Příbram. These hypotheses were not confirmed statistically. The second aim investigated the main reason for the possible lack of interest in extra vaccination of children from 0-3 years in Příbram. The target was filled with hypothesis H4: The main reason for the possible lack of parents´ interest in extra vaccination of children from 0-3 years is high price of vaccines, which was statistically refuted. The third goal was to explore parents' knowledge about diseases against which the extra vaccination of children from 0-3 years is offered. To this goal was set the hypothesis H5: Parents have sufficient information about the diseases against which the extra vaccination of children from 0-3 years is offered. This hypothesis was confirmed. The hypotheses were verified by ?chi-square? test at a significance level of 5%, which is an instrument of verification or falsification of hypotheses. This work may be used in practice as a preview to the parents´ awareness and interest in extra vaccination of children from 0-3 years. As well as cumulative information materials for professionals and the public about the above-standard vaccination of children from 0-3 years.
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