Original title: Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy
Authors: KOČOVÁ, Pavlína
Document type: Master’s theses
Year: 2017
Language: cze
Abstract: This study is focused on changes in protein expression in a glioblastoma cell line during infection with tick-borne encephalitis virus. Newly synthesized proteins were distinguished from previously synthesized proteins using bioorthogonal chemistry (BONCAT method) to observe changes in protein synthesis. Labelled proteins were visualized using two-dimensional PAGE and western blotting followed by Click reaction on membrane. Differences in protein pattern between control and infected cells were observed.
Keywords: 2D PAGE; bioorthogonal chemistry; BONCAT; tick-borne encephalitis; 2D PAGE; bioortogonální chemie; BONCAT; klíšťová encefalitida
Citation: KOČOVÁ, Pavlína. Sledování exprese proteinů v savčích buňkách infikovaných virem klíšťové encefalitidy. České Budějovice, 2017. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/40494

Permalink: http://www.nusl.cz/ntk/nusl-317333


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses