|
|
Developement and validation of method for ferulic acid isolation from wheat bran
Nábělek, Jakub ; Obruča, Stanislav (referee) ; Pořízka, Jaromír (advisor)
This master thesis deals with an optimization of processes of isolation of ferulic acid from wheat bran. Process of isolation based on alkaline hydrolysis from the substance and its adsorption was tested. In the theoretical part were described wheat bran, adsorption proces and used methods. For adsorption were tested five types of adsorbents. Especially these adsorbents: activation carbon (2,53 ± 1,16 mg.l-1), Amberlyst A-21 (105,73 ± 11,87 mg.l-1, Amberlit XAD-16 (241,55 ± 10,42 mg.l-1), Amberlit IRA-900Cl (5,90 ± 0,68 mg.l-1) and Amberlit IRA-96 (189,16 ± 6,49 mg.l-1). As the most efficient adsorbent was determine Amberlit XAD-16. The whole process has underwent detailed optimization in order to maximize the purity of the final product. The isolate was characterized by HPLC and FTIR techniques for ferulic acid and related phenolic acids. Based on FTIR analysis was discovered that the isolate was contamined by proteins. A purification procedure based on deproteination of the raw material before the hydrolysis itself was proposed. As the ultimate solution that eliminated the presence of proteins in the isolate was determine ultrafiltration with capture of molecules with Mw > 10 KDa. Ferulic acid yield from one kilo of wheat bran was 1,00 ± 0,22 g.
|
|
|
Developement and optimization of the method for the extraction of ferulic acid from wheat bran
Hubačová, Klára ; Štursa, Václav (referee) ; Pořízka, Jaromír (advisor)
Bachelor thesis is considering usage of wheat bran for an extraction of ferulic acid. Wheat bran is a large-scale by-product of milling industry which is produced during milling of cereals (wheat) into flour. This low-cost product is usually used as a livestock feed. An initiative is raised for some other potential applications, because too large quantity produced every year can not be consumed just as a feed. There is a significant amount of phenolic acids from which ferulic acid is the most abundant one. It was found out after examination of structure of the wheat bran. This acid is connected with polysaccharides of a cell wall. The alkaline hydrolysis was used to disrupt a linkage between ferulic acid and polysaccharides. Then ethanol was added to precipitate the polysaccharides and the mixture was filtered. The filtrate contains ferulic acid which has great health benefits such as anti-inflammatory, antioxidant, anti-carcinogenic, antimicrobial effect etc. It plays an important role as an agent against UV radiation in the cosmetic products.
|
guest ::
