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SMV-2019-12: Morphological analysis of miniature painting
Neděla, Vilém
Miniature painting were analysed in experimentally reached and optimised high pressure conditions of environmental scanning electron microscope and using light microscopy. Optimal gas pressure and humidity were set to minimise sample damage. Special detectors were used for low dose imaging of surface morphology.
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Analysis of Whirling of Gas Flow within the Specimen Chamber of ESEM
Bayer, Robert
The usage of an Environmental scanning electron microscope (ESEM) brings the possibility of studying many kinds of materials and chemical reactions never studied before. In this article there are compared and analyzed several simulation results of gas flow in the specimen chamber of ESEM with high gas pressure throughout the whole volume except on its optical axis, where low gas pressure is needed.
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FLOW ANALYSIS OF THE PRESSURE IN THE EXPERIMENTAL CHAMBER OF THE DIFFERENTIAL PUMPING
Šabacká, Pavla ; Bílek, Michal (referee) ; Maxa, Jiří (advisor)
The thesis deals with the effect of the critical flow, which is produced at pumping of the differential pumping chamber of the Environmental Scanning Electron Microscope (ESEM). The thesis compares the state of the flow in the area of pumped chamber at pressure ratio on the apertures when the critical flow occurs and its advantages in design of the chambers versus the flow without the clogging the nozzle. Problematics were solved using finite volume method by Ansys Fluent system.
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In vivo study of diatom assemblages using low temperature method for ESEM
Tihlaříková, Eva ; Neděla, Vilém ; Fránková, Markéta
Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.
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Nanofibrous Separators for Lithium-Ion Batteries
Pléha, David ; Míka, Martin (referee) ; Janderka,, Pavel (referee) ; Novák, Vítězslav (advisor)
Nanofibrous separators use in lithium-ion batteries brings many advantages. In contrast to contemporary used commercial separators, nanofibrous ones exhibit higher temperature resistance,ionic conductivity and higher electrolyte uptake. Better ionic conductivity is ensured by porous structure and large specific surface. Fibers creates channels for the ionic species motion. Amorphous texture of nanofibers allows quick lithium ionic species motion within the polymeric matrix of separator. Furthermore, these separators exhibit higher volume of uptaken electrolyte. Further advantage of electrospinned nanofibrous separators are both high porosity and chemical stability.
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Analysis of the gas flow in the passage of the primary electron beam differentially pumped chamber depending on the shape of the nozzle inlet
Hladký, David ; Vaculík, Sebastian (referee) ; Maxa, Jiří (advisor)
It is necessary to separate sample chamber with pressure up to 2000 Pa and the surroundings of tube with pressure 0.01 Pa with help of a differential pumped chamber at the environmental scanning electron microscope This differential pumped chamber is to be designed so that it has to manage to separate sections with very different pressures sufficiently and in the trajectory of transitting (overflying) prime electrons the average value of pressure and density has to be as low as possible, so that it would occure the lowest diffusion of these electrons, which is caused by collisions of electrons and air molecules too. It were created axisymmetric 2D models variant of Differentially pumped chamber with different opening angles of the clone, which were analyzed to choose the best variant. The Ansys Fluent systeme using finite volume method for analysis of fluid flow was used for analysis.
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Methods of imaging and analysis of biological specimens
Novotná, Veronika ; Tománek, Pavel (referee) ; Knápek, Alexandr (advisor)
Bachelor thesis deals with characterization of human tissues and bacteria from the microscopic point of view. Physical principles of SNOM and electron microscopy (TEM, SEM, ESEM), together with their suitability for observing of biological specimens, were presented. In the next section, there is focus on data transformation from electron microscopy from time plane to image plane and their following processing. Next section is about experimental preparation of specimens for observing in SEM, ESEM and SNOM. Currently there is an idea to use scanning near-field optical microscope for biological research. That is why the goal of this thesis is compare SNOM and EM techniques, mentioned above. In this thesis we try to use SNOM as a cheaper option than EM in the observation of biological specimens. EM and SNOM allow obtaining different, however comparable and complementary information of the specimen.
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Detection of signal electrons in ESEM
Šváb, Martin ; Špinka, Jiří (referee) ; Jirák, Josef (advisor)
This bachelor's thesis deals with problems of environmental scanning electron microscopy and with detection of signal electrons at higher pressure in working chamber. In this thesis I will focus on comparison of detected signal level achieved under different working conditions.
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