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Detection of neuronal pathways after CNS lesions by using dextran amine.
Muchová, Zuzana ; Konopková, Renata (referee) ; Otáhal, Jakub (advisor)
Title of work: Detection of neuronal pathways after CNS lesions by using dextran amine. Aim: Develop a metodology for pathway tracing with the substance transmitted by axonal anterograde transport and compare iontophoretic and pressure injection of BDA. Methods: The experiment was performed on adult rats which where injected iontophoretic and pressure anterograde tracer BDA. One week after injection were decapitated in deep anesthesia, their brains removed, frozen in dry ice and stored at -70řC. The brains were then cut into thin coronal slice (50 µm), immunolabeled and loaded on microscopic slides. Individual sections were examined labeling nerve fibers at the injection site, its surroundings and contralateral side of the hemisphere. Results: After injections of anterograde tracer BDA into the nervous tissue have been shown labeling of nerve fibers at the injection site, surroundings and contralateral side of the hemisphere. As an efficient method for its injection was determined according to results of the pressure method. Key words: anterograde tracer, BDA, iontophoretic injection, cerebral ischemia, neuroplasticity
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Využití vybraných zemědělských a potravinářských odpadů k výrobě funkčních potravin a biomateriálů
Urban, Marián
This dissertation thesis describes utilization of agricultural and food processing waste and intermediate materials for manufacturing of functional food and bio-materials. The aim of this study is to develop new procedures to process food and agricultural byproducts in order to create modern products and materials with higher added value. The focus here is especially on food supplements, nutraceuticals, bio-materials for health industry and pharmacology. Examples can be substances for drug delivery or substances for controlled release of active ingredients. Examples of such products are cyclodextrins, chitosan, chitin-glucan complex and glutathion.
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Stanovení biologicky aktivních substancí v rostlinném materiálu
Dobeš, Jiří
This thesis is focused on the determination of antioxidants and antioxidant activity. In the first work DMPD method was optimized to determine the antioxidant activity of antioxidants standards and the reaction was monitored depending on the time, temperature and concentration. In the second work automated determination of antioxidant activity of phenolic compounds standards rutin, quercitrine and standards of phenolic acids gallic acid and ferulic acid was tested using five different methods used in the determination of antioxidant activity using an automated pipetting machines and spectrophotometer. In the third work phenolic profile and total antioxidant capacity (TAC) was determined in apricot cultivars with methods high frequency liquid chromatography with tandem electrochemical and UV spectrometric detection and these two methods were compared. In the fourth work electrochemical methods used for the determination of phenolic compounds were described. In the fifth work oxidative stress induced by silver ions in the Staphylococcus aureus bacterial culture was investigated using spectrophotometric and electrochemical methods.
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Porovnání fluorescence přírodních humusových látek
Pořízková, Karla
Diploma thesis "Comparison of natural humic substances fluorescence" is aimed at identification of soil humic substances and natural humic substances using fluorescence spectroscopy. Results showed that fluorescence of humic acids isolated from lignite was similar to soil humic acids and was registered at longer wavelength. On the other hands lignohumate had higher fluorescence intensity at shorter wavelength. Fluorescence spectroscopy is a useful tool for identification of humic substances isolated from different sources.
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Financial analysis of Stavby silnic a železnic, a.s.
Vágner, Jan ; Boukal, Petr (advisor) ; Zelený, Miroslav (referee)
Diploma work is engaged in financial analysis of Stavby silnic a železnic, a.s. company. The aim is to pass judgment on company's present financial situation and its progress from year 2001. It is focused on important facts concerning financial health. It warns of possible threats and try to define some recommendations to achieve stability and prosperity. It utilizes theoretical knowledge of financial analysis like horizontal and vertical analysis of financial statements, rules of financing, ratio indicators, pyramidal system of indicators, working capital analysis, basic economic inequality, EVA method and bankrupt models. Company is also compared with a number of transport building companies.
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The city of Zlin in the whirl of political changes during 1899-1914
Škrabala, Petr ; Dobeš, Jan (referee) ; Ebelová, Ivana (advisor)
The main goal of this work was to describe a background of politi cal combat between the so-called clerical and progressive part of population in Zlin in the first decade of 20th century and also to focus on the achievements of the progressive council, led by the mayor Frantisek Stepanek. The town expanded dramatically during this period. Zl in population was suddenly rising and also the importance ofthe town as a natural centre of the region grew in a clo se connection with the economic progress (foundation ofthe Bata Company and other shoemaking firms). The victory of the progressive groups in local election in 1908 was very important as it brought a release of the tension into Zlin' s politi cal life on the one hand and it enlivened the importance of the town as a political, administrative, economic and transport centre on the other hand. It was achieved to establish a district court based in Zlin, the new council was working on improvement of the life standard of Zlin inhabitants. A council power station was built, new roads connecting Zlin with surrounding villages were constructed, a telephone connection was established, a sewerage system was developed and streets in the town were cobbled ... Fundamental steps were taken to start works on a regulation of the Drevnice River and on a local railway...
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Nicotinism as a predisposition to the use of illegal addictive substances.
NOVÁ, Iveta
Nicotinism as a predisposition to the use of illegal addictive substances Bachelor Dissertation Abstract The goal of my Bachelor Dissertation is to map out and analyze how tobacco products and other addictive substances are used, to determine the dependence between the use of nicotine and other addictive substances and to identify the risk factors which lead to the use of these substances in the population of basic school pupils. In the theoretical part I address the concept of tobacco, its composition, dependence and the consequences of its use. I then describe the term drug addiction, its origin, development and stages. In the conclusion I characterise the period of adolescence and the risk factors and differences concerning addictive substances which are connected with it. To meet the set goals, quantitative research was chosen, a method of questioning, using anonymous questionnaires, which were employed to define pupils' experience with tobacco and other addictive drugs. Data was collected from the 9th years of basic shools and from the corresponding years of 8-year grammar schools. For the research, two assumed hypotheses were designated and opened. Hypothesis 1: The users of tobacco products have more extensive experience with illegal addictive substances than non-users of tobacco products. This hypothesis was verified. Hypothesis 2: Girls use more tobacco products than boys. This hypothesis was also verified. The results of my Bachelor Dissertation can be used for educational purposes, as part of preventive activity in educational institutions and for making this more effective, for further research and also as material for improving awareness of this issue.
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Free time activities in civic association Český sedlák with regard to health limitations of drug users
BRODSKÝ, Dušan
The thesis deals with the evaluation of situation on the drug scene in Europe and Czech Republic. Introduces basic concepts related to health limitations of drug users and other addictive substances users. It works with clients potential health limitations. It addresses health and its importance for life. Describes basic components of free time. It offers questions for clients. Proposes the basic methods of work in free time. Animation as the most suitable method.
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Preventive programs of addiction at the seconder school
FILOVÁ, Aneta
The bachelor thesis is focused on problems of addictive substances and prevention of these problems in the area of school education. The objective of this thesis is to find on empirical investigation basis, under what circumstances and how much does the use of addictive drugs occur in student?s society. The theoretical part of bachelor thesis analyzes drug problems and discusses drug addiction reasons. The practical part of bachelor thesis is based on proper empirical investigation, where the high school students were asked.
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