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Comparison of various ways of tick infection with the tick-borne encephalitis virus
VÝLETOVÁ, Eva
The aim of the bachelor thesis was to compare various methods of Ixodes ricinus tick infection with the tick-borne encephalitis virus. For infection by immersion method, less virulent TBE virus strain Neudoerfl was used resulting in 5 % of infected ticks. Using more virulent strain Hypr provided 60 % of infected ticks. 75 % of ticks became infected with the Neudoerfl virus by capillary feeding. Infection of ticks by feeding on viraemic mice was unsuccessful. Applicability of tested methods for infection of tick life stages is discussed.
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New potential antivirals against tick-borne encephalitis virus infection
VESELKOVÁ, Barbora
The experimental work of the thesis was concentrated on studying of the inhibition effect of twenty-six chemical substances on tick-borne encephalitis virus. The substances showing inhibition effect in High Throughput Screening assay were processed to Cytotoxicity assay, where their toxic effect on tissue cell cultures was determined. Subsequently, substances with inhibition effect and no cytotoxic effect were incubated with cell culture and virus in two different incubation time periods and the supernatant containing virions was examined using plaque assay.
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Vector-pathogen-host interaction on the example of spirochetes Lyme boreliosis disease (and tick-borne encephalistis virus)
VAVRUŠKOVÁ, Zuzana
This study was focused on vector-pathogen-host interaction. Questing ticks from field were tested for presence of Borrelia burgdorferi s.l. and host DNA. Small rodents were trapped, ticks were collected from them, infestation patterns were estimated regarding the species and stage of ticks and species, sex and body weight of the host. Ticks aquired from hosts were tested for presence of Borrellia burgdorferi s.l. and tick-borne encephalitis virus. Both results from identification of hosts and from detection of pathogens were compared to be able to investigate interactions between host, vector and pathogen.
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The use of negatve staining method for viral and bacterial diagnostics using the transmission electron microscope.
ŠOBEROVÁ, Tereza
This bachelor thesis is focused on the negative staining method used in making diagnosis of viruses and bacteria in the transmission electrom microscope (TEM). Theoretical part provides general description of TEM, negative staining method and basic characteristics of viruses used in this project. Detailed description of procedure designed for the preparation of specimens is the key part of this project. After their display in TEM, the testis analyses the quality of virus images and comparison of effects of the most common staining agents, pH, trehalose and different times of staining on displaying the ultrastructure of biological specimens and on the size of individual viral particles. The conclusion of project contains staining agents providing the best imaging of objects that have been selected based on the evaluated results and recommendations for potential enhancement of staining properties in other agents.
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Ecological and epidemiological aspects of tick-borne encephalitis
GREGOROVÁ, Eva
Tick-borne encephalitis (TBE) virus is endemic in many countries in central, eastern and northern Europe. Nowadays its importance rises because of its increasing incidence. This study focuses on characterization of tick-borne encephalitis virus, its host and human disease caused by this pathogen and efficacy of available vaccines. Furthermore, an evaluation of climatic changes and their influence on TBE incidence is discussed.
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Complete genome sequence analysis of TBE virus, strain Hypr, in different passages in host \kur{in vivo}.
ŠIMONOVÁ, Zuzana
I have compared genomic sequences of three variants of tick-borne encephalitis virus, strain Hypr. The variants used were Hypr5 {--} virus passaged five times in suckling mice brains, Hypr50/V {--} a variant highly passaged in vivo in Vienna, and Hypr50/CB {--} a variant highly passaged in vivo in České Budějovice. Both high-passage variants were passaged in suckling mouse brains as well. I was interested in changes in nucleotide sequence of genomes of high-passage variants Hypr50/V and Hypr50/CB compared with Hypr5 and also consequential changes in amino-acid sequences of viral proteins. I have found several identical changes in amino-acid sequences of E protein, NS3 and NS5 proteins in both high-passage variants and also a number of substitutions unique to one of the variants in proteins E, NS1, NS2A, NS2B, and NS3. A nucleotide substitution was found also in 5{\crq}UTR of the viral genome.
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