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Preparation and characterization of superparamagnetic inorganic/polymer particles for biomedical application
Zasońska, Beata Anna ; Horák, Daniel (advisor) ; Šafařík, Ivo (referee) ; Sysel, Petr (referee)
Superparamagnetic -Fe2O3 nanoparticles were synthesized by coprecipitation of ferric and ferrous salts with a base. Resulting nanoparticles were coated with shells, such as poly(N,N- dimethylacrylamide) (PDMAAm), neat and functionalized silica (SiO2 and SiO2-NH2), and polyaniline (PANI). PDMAAm shell was introduced by modification of iron oxide nanoparticle surface with an initiator and N,N-dimethylacrylamide was polymerized producing -Fe2O3&PDMAAm core-shell particles. In case of SiO2-NH2 shell, tetramethyl orthosilicate was used to yield -Fe2O3&SiO2 nanoparticles, which were subsequently modified by (3-aminopropyl)triethoxysilane to prepare γ-Fe2O3&SiO2-NH2 particles. Oxidation of aniline hydrochloride with ammonium persulfate in an aqueous solution of poly(N-vinylpyrrolidone) in the presence of iron oxides produced -Fe2O3&PANI nanoparticles. Finally, the last type of the particles was based on thionin-modified poly(carboxymethyl methacrylate) (PCMMA&Th). The particles were characterized by techniques, such as scanning and transmission electron microscopy (SEM and TEM) and dynamic light scattering (DLS) to determine the particle morphology and hydrodynamic diameter. The presence of the functional groups, chemical composition, and the iron content were investigated by Fourier-transform...
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The prognostic significance of molecular markers of acute myeloid leukemia on the outcome of allogeneie hematopoietic stem cell transplantation
Karas, Michal ; Holubec, Luboš (advisor) ; Faber, Edgar (referee) ; Žák, Pavel (referee)
In this thesis entitled " The prognostic significance of molecular markers of acute myeloid leukemia on the outcome of allogeneic hematopoietic stem cell transplantation " the author deals with the following issues: 1. In the literature overview: the author characterizes the epidemiology, patogenesis, diagnostics, prognosis and treatment of acute myeloid leukemia, focusing on evaluation of different prognostic markers in relation to the treatment of acute myeloid leukemia mainly using allogeneic hematopoietic stem cell transplantation. 1. The methodological section characterizes the following: a) Groups of patients and methods of clinical evaluation in: Detailed characteristics of 60 patients with acute myeloid leukemia with normal karyotype and NPM1 gene mutations in complete remission who underwent allogeneic hematopoietic stem cell transplantation on Haematology and oncology department of University Hospital Pilsen in the years 2005-2014. The evaluation of other parameters of the underlying disease, and a detailed description of transplant procedure (donor selection, the type of pre-transplantation preparation etc.) is also part of characteristics. b) The timing and method of sampling for evaluation of the level of minimal residual disease before transplantation. Description of the method for...
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Signalling of protein kinase B and expression of cyclooxygenase in early phases of diabetic nephropathy
Ždychová, Jana ; Komers, Radko (advisor) ; Haluzík, Martin (referee) ; Matoušovic, Karel (referee) ; Škrha, Jan (referee)
Introduction: Renal hypertrophy, extracellular matrix accumulation, aItered apoptosis as well as changes in regional hemodynamics have been implicated in the pathophysiology of nephropathy in diabetes mellitus (DM). On the molecular level the detailed mechanisms for development of diabetic nephropathy (DN) have becn intensively studied. Insulin induces a variety of biologicaI effects in a number of cell types via phosphatidylinositol-3 kinase (PI3K)/Akt kinase signaling pathway. Considering multiple function of Akt that incIude potentiaIly hannful pro-growth effects mediated by mTOR and cyclooxygenas-2 (COX-2), as well as protective effects mediated by endotheliaI nitric oxide synthase (eNOS), it is possible that aIterations in activities of Akt may play role in the pathophysiology ofDN. Renal corticaI activity and expression of Akt, its down-strearn effectors mTOR, eNOS, and "C<JIX-:Z. as well as PTEN, an endogenous Akt inhibitor, were investigated in streptozotocin (STZ): diabetic rats as a model of Type 1 DM with different levels of glycenůc control, and in Zucker ~d.iabetic fatty rats, a model ofDM2, and in nondiabetic rats as controls. Methods: Akt activity was measured by kinase assay. Protein expressions were measured by .immunoblotting and immunohistochemistry in renal cortex of 4- and 12- week old...
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Charity Projects of Organizations Providing Care for Children with Disabilities in South Bohemia
LANDOVÁ, Lenka
The bachelor thesis "Charity Projects of Organisations Providing Care for Children with Health Disability in the Region of South Bohemia" deals with those projects that were founded by non-profit organisations and that provide care for children with health disability in the Region of South Bohemia. The aim of this thesis is to chart the charity projects of above mentioned organisations and to find out what is a proper and effective way to create them and what methods and procedures are used by non-state, non-profit organisations. The first research inquiry refers to a successful implementation of these projects. The result will be not only a realisation what kind of projetcs were created by the above mentioned organisations but also what is important for an organisation itself. Within the bounds of the second research inquiry I find out wheather there is an optimal procedure for the implementation of the charity projects of non-state, non-profit organisations. A qualitative method was used for a research part of this thesis. I used a method of a half-structured interview, a technique of a personal interview. A research group was selected for bringing the research into practice, which includes nine organisations seleceted from non-state, non-profit organisations providing a care for health disabled children in the Region of South Bohemia. It is more difficult for non-state, non-profit organisations it this field to obtain enough finances for providing services and for obtaining necessary aids than for state non-profit organisations because of different ways of fundraising . A non-state, non-profit organisation has several possibilities how to obtain necessary material and non-material sources. There are charity projects that offer means of selfless help; provided they are successful, they help the organisation to obtain necessary sources. It emerged from the research that it was very important for non-state, non-profit organisations to get a multi-source fundraising and supporters of their projects, mainly donors and sponsors, to obtain available care for children with heath disability. On the basis of the research, it is possible to state that non-state, non-profit organisations providing care for children with health disability, implemented many successful projects. The results of this thesis can be used in the way of an informative material for setting a complete view of a solution of the problems of the above mentioned organisations projects.
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3D scanning of glossy surfaces
Zeman, Jakub ; Zatočilová, Aneta (referee) ; Koutecký, Tomáš (advisor)
The goal of this bachelor thesis is to experimentally determine the impact of different concentrations of titanium matte coating on the properties of matte film covering glossy surface intended for 3D scanning. Theoretical part of the thesis contains brief introduction to the 3D digitization of real objects as well as more detailed description of the particular scanning method used in this thesis and summary of possible digitizing errors and existing knowledge about matte coatings. The practical part composes of applying the titanium matte coating on specimens using automated coating device thus maintaining the exact same conditions for all concentrations. Following measurement of specimens using 3D scanner and optical profilometer results in determining the optimal concentration of titanium coating providing the best properties of the matte film.
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Effect of colloidal silicon dioxide on selected properties of cement pastes and mortars
Barek, Jaroslav ; Žižková, Nikol (referee) ; Rovnaníková, Pavla (advisor)
The presented diploma thesis deals with the potential use of colloidal silica in cement composites. Investigation is focused primarily on selected properties of cement pastes and mortars with colloidal silica content. Two selected types of colloidal silica (particles with average size of 14 nm) have been used as a cement addition and partial replacement of cement, respectively. The experimental program for cement pastes with colloidal silica included tests for isothermal calorimetry (the study of cement hydration) and thermal analysis (determining the portlandite content). Hardened cement pastes have been studied through measurements of compressive strength. The microstructure was examined by scanning electron microscopy. Hardened mortars with colloidal silica have been studied through measurements of compressive strength, flexural strength, modulus of elasticity and fracture mechanics parameters. Fracture mechanics tests show that 5 % and 20 % addition of colloidal silica can enhance after 28 days of curing fracture energy up to 18,4 % and 32,7 %, respectively. For the compressive strength enhancing effect of colloidal silica, it was found to be more pronounced in the early age, while rate of strength gain can be lower than the control in the later ages. Our investigations revealed that the silica sol will coagulate immediately when the cement is mixed into the water containing sol. The ionic composition of pore fluid significantly influences the stability of colloidal silica and lead to their aggregation. After additional tests it has been found that small addition of calcium hydroxide greatly improved the compressive strength of the resulting cement pastes with colloidal silica content. Addition of calcium hydroxide in conjunction with modified cement paste preparation can enhance compressive strength after 3 days of curing up to 64,4 % in comparison with the blank paste.
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Economic rationale of private donors
Miklovičová, Katarína ; Hlaváček, Jiří (advisor) ; Turnovec, František (referee)
The aim of this thesis is to analyze motivation of companies to support social, environmental and development projects. The analysis is focused on Central European companies, namely the Czech Republic, Slovakia, Hungary and Poland. In the empirical part of the research interviews with selected companies and non-profit organizations are conducted. By interviewing both sides we can see how both donors and main recipients view the motivation behind the support. Afterwards, the relationship between supporting social, environmental and development projects and long term financial performance is examined. Coefficients are assigned to each company according to their involvement in the three categories - social, environmental and development. As measures of financial performance we choose stock market returns, which are then regressed on the three coefficients and selected control variables. The regression results show that supporting development projects tends to have a positive influence on companies' stock market performance. We do not confirm that supporting social and environmental projects improves stock market performance of companies. This outcome suggests that supporting development projects is a win-win situation for both companies and non-profit organizations. Companies can improve their...
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Risks of organism intoxication related to chemical substances commonly used in households or public places
Drápal, Stanislav ; Neklapilová, Vlasta (referee) ; Adamec, Vladimír (advisor)
The thesis is focused on the possibility of intoxication by selected chemicals at home or in public places. Especially the danger of carbon monoxide poisoning. We’re assessing the current state of the level of awareness and prevention in the Czech Republic. The thesis prepared an overview of the current state of intoxication busy the carbon monoxide in the Czech Republic and the level of medical procedures. There are evaluated measures, which are aimed to reduce the frequency of poisoning. The outcome of the thesis is to design a set of measures, which are designed to eliminate health risks to the population and raise public awareness. The thesis was carried out their own research facilities, household detectors, carbon monoxide at a pre-selected location.
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