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T cells labelling by bimodal contrast agent for in vivo studies of stroke
Krijt, Matyáš ; Poljaková, Jitka (advisor) ; Kříž, Jan (referee)
Stroke is a serious brain injury, which causes sudden death or terminates in permanent neurological disability. Nowadays, tissue plasminogen activator (tPA) is used as the only effective treatment of stroke. One of the potential targets for novel therapy are T cells. Even though the explicit role of T cells in the pathogenesis of brain injury, amounts and timing of all T cell subtypes infiltrating into brain during the stroke still needs further investigation. The research in this field is complicated by the lack of efficient methods for in vivo cell tracking. Therefore the aim of this thesis was to develop a method of T cells labelling by MRI contrast agent in order to investigate T cells distribution in ischemic mice model using in vivo MR imaging. T cells were isolated from C57/BL6 mice in two step isolation protocol using gradient centrifugation and magnetic separation with the efficiency of 97 %. The isolated cells were labelled with 100 μg Fe/mL of Molday ION Rhodamine B contrast agent. The labelling efficiency after 17 hours of cells incubation was higher than 99 %. The labelled cells were cultured with CD3 and CD28 antibodies resulting into the 74 % viability of labelled T cells compared to 83 % viability of non labelled T cells. The labelled T cells were visualized by fluorescent...
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Výběr a implementace open source nástroje pro řízení portfolia projektů
Marek, Jan ; Chlapek, Dušan (advisor) ; Kučera, Jan (referee)
Methods and ways of implementation of changes and innovations in companies through project management are in today's society very well established. There exists methodologies, techniques and tools for the management of individual projects. However in a role of project manager, I very often faced the fact that companies are performing the project portfolio management in very intuitive way. This in itself leads to failed and prematurely terminated projects, initiation of the wrong projects or realisation of correct projects, but at the wrong time. Very often I have also recognized, that there is lack of awareness of fact, that there are also Open Source applications that can help with the organization's portfolio. This thesis deals with the definition of requirements, search and selection of proper OSS application and subsequent implementation. In the first part of this thesis I prepare a theoretical framework about PPM, and on that basis then identify and verify a set of requirements for selection of right OSS PPM application. The next section of thesis describes searching for suitable applications from a variety of sources, assessment against the requirements and the final selection. In the last part the implementation project is drafted, which aims to serve other colleagues in project management as one of the possible implementation paths. The outputs are then continuously confronted with experts in matter of projects and project management in the field of IT in order to maximize symbiosis between theory and real life experiences. Virtually every project management methodology defines the collection of already-proven techniques, best practices or lessons learned from the previous similar implementations. The main contribution of this thesis I see in fact that it contains not only the design of the project implementation, but also describes a logical path, what leads to the result. Therefore this thesis could be used as a base or discussed best practice, when a project of implementation of PPM application in place.
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Supported Emplayment from the Perspective of Emplayers
MYŠÁKOVÁ, Hana
The philosophy of supported employment in the Czech Republic has been developing for over than twenty years. Nevertheless this service is still neglected and only a few non-profit organizations put it into practice. The service is not legislatively based and also the society does not have a clear opinion on employment of people with disabilities. Current labour market situation is not advantageous at all. Therefore some support of disabled people looking for a job is becoming more necessary and required. The employers are an important part of the whole problems, because their attitude to the idea of supported employment plays a significant role. The bachelor's thesis "Supported Employment from the Perspective of Employers" has pretentions to look into supported employment from the employers' point of view. They are not active initiators of the service, but they are important participants for its successful realization. The theoretical part of the thesis tries to describe the basic problems. It covers the service of supported employment from historical development to its importance. It focuses on special characteristics of disabilities and their social impact on human beings. The closing part is dedicated to working opportunities for people with disabilities in the circumstances of current legislative and economic situation. The practical part is written in the form of a qualitative research. The method of questioning and semi-structured interviews were used for the purposes of the research. Eight people were chosen intentionally for the survey. The data gained from the interviews were analysed with the method of open coding. The main aim of the thesis was to map out empoyers' experience with the service of supported employment. Motivation of employers to put this service into practice was the next part of the research. The question was: "What reasons for realization of supported employment do predominate by employers?" The research showed the most frequent facts, which predestine the integration possibility of disabled people in open labour market fundamentally. After the interviews and answering the questions of the research, it was possible to describe the employers' experience with the supported employment service and also their main incentives for its realization. It was found out that employers' experience with the supported employment service is quite limited. Employers often get to know it through the agency for supported employment, which is not only the initiator of cooperation, but it also plays an important role when a disabled person is being employed. An important point of successful employment is often how the emploeyer is satisfied with the agency. The employment of disabled people often brings certain risks connected with the limits of disabilities. Employers, who enable disabled people to work, are aware of that. The impulse for employment can vary from financial advantages, company philantrophy to personal solidarity of the employer. Social responsibility of the company is becoming a trend, that many employers start to know. But it can be presumed to influence the employment of more people with disabilities and at the same time the looking up the supported employment service by the employers. This bachelor's thesis can be contributive to understanding the importance of employment for disabled people's living. It brings information about the employers' experience with the realization of supported employment and motivation to the realization. It can also help to uncover integration obstacles of people with disabilities in open labour market.
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The Oriental Institute in Prague: Cultural Department and the Orient
Jůnová Macková, Adéla
A substantial contribution to Oriental studies was founded with a new institution, the Oriental Institute. The idea was promoted by the Czechoslovak president T. G. Masaryk and by his advisor in Oriental politics, Alois Musil. The institute was financially supported by the ministry of Foreign trade, the main role played Minister Rudolf Hotowetz (first director of the Oriental Institute). The Oriental Institute was established in 1922 and financially and functionally stabilised in 1927/1928. There were no permanent salaried posts; however, the cultural section of the Institute financed publications and travel by means of fellowships and grants.
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Study of the composition and organization of cytochrome P450 system by covalent crosslinking
Koberová, Monika ; Hodek, Petr (advisor) ; Hansíková, Hana (referee)
The system of mixed function oxygenase (MFO system) participates in significant roles in the metabolism of endogenous compounds and xenobiotics. This system contains cytochrome P450, NADPH:cytochrome P450 reductase, and also there are assigned NADH: cytochrome b5 reductase and cytochrome b5. It was proved that cytochrome b5 can stimulate or inhibit cytochrome P450 (CYP)-dependent reactions and even change the ratio of resulting metabolites. The mechanism of cytochrome b5 action has not been fully elucidated yet. Elucidation of protein-protein interactions in MFO system and determination of topology of this system could explain the mechanism of cyt b5 action. The covalent cross- linking technique is suitable method for identifying protein-protein interactions within the membrane. Cytochrome b5 contains 3 methionines and in 2 cases the methionines are localized in a short hydrophobic C-terminal membrane anchor. Interactions with cytochrome P450 in the membrane environment can be identified by substitution of two methionine for photoactivatable analogue of methionine (photo-methionine) and subsequent photoactivation. This work is focused on expression and isolation of photo-cytochrome b5 (photo- cyt b5), cytochrome b5 analogue with incorporated photo-methionine. Conditions for photo-methionine...
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Eating habits of patients with metabolic syndrome
NOVÁČKOVÁ, Anna
Metabolic syndrome (syndrome X, Reaven's syndrome) is frequently occurring disease in Czech Republic. The diagnosis often detected only separated parts of metabolic syndrome which are usually not connected with complex of MS. Medical conditions co-occurred within this syndrome are following: elevated fasting plasma glucose (insulinoresistance), abdominal (central) obesity (women waistline > 88 cm, men waistline > 102 cm), elevated blood pressure, high serum triglycerides (> 1,7 mmol/l), and low high-density cholesterol (HDL) levels (women 1,25 mmol/l, men 1,0 mmol/l). The incidence of MS can be divided into the prevalence of MS itself (this correspond with 30%) and the prevalence of each individual part of MS which correspond with 80% population. The most frequently reasons of MS are so called environmental impacts as e.g. central obesity, insulinoresustance syndrome, fatty liver, systemic inflammation, intestinal alterations, hypertension, hypertriglyceridemia, foetal malnutrition, low basal energetic outcome, sleep apnoea syndrome, changes of gut flora, hyperglycaemia and cumulation of visceral fat. This bachelor´s work is concerned with eating habits, life style and other factors which could be reason of described metabolic syndrome. The aim of this study is evaluation of eating habits and life style of patients suffered from MS before syndrome outbreak, as well as participation of obesity in MS outbreak assessment. Two explorative questions were formulated on the basis of described goals: First research question: Did the MS patients follow principles of healthy eating before syndrome outbreak? Second research question: Does obesity have a significant effect on MS outbreak? Definition and characterization of MS in relation to diet is described in theoretical part of my work as well as explanation for reasons of MS outbreak, descriptions of each separated diseases of MS complex and their treatment. Metabolic syndrome in connection with obesity and its classification into each level is defined inthis part. Obesity treatment together with prevention and treatment of MS are suggested in terminal part of this section. In practical part of work, the results of own research are presented. This section is focused on: 5. evaluation of eating habits before MS outbreak; 6. obesity incidence in MS patients; 7. patient´s life style; 8. the presence of individual diseases participate in MS. Research part of my bachelor´s work is prepared with using of quantitative assessment, which was done by questionnaire investigation in southbohemian and westbohemian region. Research population consists of thirty gender-nonspecific and age various patients diagnosed as having MS. The research questions were focused on mapping of respondent´s eating habits, life style (smoking, movement activity, daily eating portions and breakfast regularity), previous and current illnesses as well as diseases of respondent´s relations. The aim of described investigation was evaluation of obesity, life style and diet participations in MS outbreak. The results did not prove observance of healthy eating principles in MS patients before and after syndrome outbreak, while most of respondents were overweight or obesity. It can be assumed that genetic predispositions play role in MS outbreak. Additionally, very interesting is detected occurrence of MS in many individuals younger then forty years old. The present work extended the knowledge about risk factors of MS outbreak together with eating habits, obesity and MS outbreak connections. When reasons of MS seem to be more clear, it is easier to establish new more effective prevention. On the basis of the results, it can be arranged diet suggestions useable as effective prevention of MS outbreak.
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Miřenka Čechová
Janečková, Kateřina ; PETIŠKOVÁ, Ladislava (advisor) ; SOPROVÁ, Jana (referee)
Monographic work focuses on artistic creation Miřenka Čechová, one of the
the leading personalities of our contemporary stagecraft. Her personal and
artistic development started when she began studying dance and continued to
mimic and physical theater. Material presents her contribution to
contemporary Czech scene physical theater and refers to her international
success. Work observes her role like co-founder of physical theaters Theatro
Pantomissimo and Spitfire Company and to presents the work of these
physical theaters and reveals the working and teaching methods in the
preparation of copyright performances.
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Mask, Disguise and Role Play in Worldwide Contemporary Self-Portrait
Helfertová, Žofie ; SILVERIO, Robert (advisor) ; DVOŘÁK, Tomáš (referee)
This bachelor thesis deals with the role of mask, disguise and playing with the roles through self-portrait in worldwide contemporary photography. In the first chapters it follows the general development of the self-portrait and a brief history of photography and its forms and tendencies from beginning to the present, it is also focused on the mask in general and role of masks and disguises in the history of photography. Main part of this work is the last chapter, which is devoted to the youngest generation of contemporary photographers. In their self-portraits we can follow tendency which reveals use of mask and disguise for change of their identity. However, they do not create a new identity but takes over, or form the illusion of existing already defined identities. The aim of this thesis is to present the mask as an instrument for role play through a self-portrait.
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