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Crystallographic study of the iron-regulated outer membrane lipoprotein (FrpD) from Neisseria meningitidis
SVIRIDOVA, Ekaterina
Neisseria meningitidis (N. meningitidis) is a Gram-negative commensal bacterium colonizing nasopharynx of about 10 % of healthy individuals, which can cause invasive diseases, such sepsis and meningitis, upon occasional penetration into bloodstream. Pathogenesis of N. meningitidis appears to be directly related to conditions of limited iron availability. Under these conditions two proteins of unknown function: FrpC and FrpD, are synthesized. FrpD is a highly conserved lipoprotein of N. meningitidis anchored to the bacterial outer membrane. It is known that FrpD tightly binds the FrpC protein, which belongs to the Repeat-in-Toxin (RTX) protein family and may act as bacterial exotoxin. However, the mechanism of FrpD-FrpC interaction and the exact function of this complex are unknown due to the absence of structural information on these proteins. Therefore, we set out to determine the structure of FrpD and provide insights into its interaction mechanism with FrpC and structure-functional relationships of these two proteins. We determined the first crystal and solution structures of the FrpD protein. We found that atomic structures of FrpD reveal a novel protein fold. We uncovered the structure-function relationships underlying the mechanism of interaction between the FrpD and FrpC proteins and tested the putative function of the FrpD-FrpC1-414 complex in vitro. Finally, we proposed the putative function of the FrpD-FrpC1-414 complex as a new minor adhesin of N. meningitidis, which mediates the bacterial adhesion to the host epithelial cells and facilitate the colonization. Our work constitutes the first step in clarifying the molecular basis of the FrpD-FrpC interaction and sets the base for further investigation of the role of FrpD and FrpC in the virulence mechanism of N. meningitidis.
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Preparation of model membranes to study their interactions with biopolymers using fluorescence correlation spectroscopy
Adamcová, Zuzana ; Márová, Ivana (referee) ; Mravec, Filip (advisor)
This diploma thesis is focused on preparation and characterization of supported lipid bilayers as simplified models of cell membranes. The bilayers were prepared from source system of lecithin liposomes in phosphate buffer using the vesicle fusion method on a cover glass sufrace hydrophilized by plasma. Three fluorescent probes – Nile red, Oregon Green DHPE and DiO – were utilized to characterize diffusion within the bilayer using fluorescence correlation spectroscopy. For this purpose Z-scan FCS, which is a method developed specially for planar samples, was used. After the process of preparation and characterization of supported lipid bilayer was optimalized, interaction between this artificial membrane and solution of hyaluronic acid in phosphate buffer was studied. It was found out, that addition of this biopolymer causes slowing the diffusion of the fluorescent probe within the bilayer.
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Design of system external communication in district of Prague 14
Novotný, Tomáš ; Pešek, Ondřej (advisor) ; Pavlík, Marek (referee)
The purpose of this work is to analyze the existing forms of external communications of municipal authority in Prague 14 and suggest improvements to modern approaches and appropriate to the needs of local residents. This area is developing dynamically in the Czech Republic. Methods that are common in the private sector are now moving into the public administration, as the new trend is to understand its performance as a service. This is related to the need for better interaction between the municipal authorities and the relevant residents. The theoretical part will summarize current knowledge on the topic. With their help, then the practical part will verify their existing application, reveal shortcomings and suggest corrective solutions. I would like to emphasize that I will not deal with internal communication of municipal authority.
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Volume Visualization of Human Skulls
Hrinčár, Vladimír ; Pelikán, Josef (referee) ; Maršálek, Lukáš (advisor)
Volume visualization is an important tool for exploration and understanding of complex 3D scientiffic data, as those acquired for example by CT scanning. Recent advancements in hardware and software renewal interest in interactive, high-quality volume rendering as it now becomes feasible even on desktop computers. In this work we present new application designed for visualization of CT scans of human skulls. We focus on image quality and add new features for skull comparison and di®erence visualization. Our solution is based on .NET application, which provides user interface(GUI) and external volume renderer as dynamic link library. We have implemented complex renderer for high-quality isosurfaces using algorithms based on interactive ray-marching methods including self shadowing and transparency. We also add support for multiple volumes and introduce new rendering modes for difference visualization. In addition our system uses NVidia CUDA technology, showing how to harness the power of modern GPUs for accelerating this complex task. Our results show that we are able to deliver interactive visualization tool, which can greatly help in areas like anthropological research.
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The preprocessor for system LISA
Hora, Petr ; Šiňor, M.
In this contribution we pay attention to the propagation of the stress waves in an arbitrary complex inhomogeneous media. For the system of elastodynamic wave equations we have adopted the method of solution based on the local interaction simulation approach (LISA) and the sharp interface model (SIM) introduced for 1D, 2D and 3D cases in [1-3].
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Electronic books at VŠE
Saska, Filip ; Koubský, Petr (advisor) ; Šperková, Lucie (referee)
The master dissertation Electronic books at VSE is focused on analysing the opportunities of e-books in the environment of VSE. The aim is to describe the current state of different study materials used at VSE and to explore different ways of improving them using e-book technology. The dissertation briefly describes various attributes of e-books with stress on the relevant attributes -- the possibility of inserting notes, bookmarks, multimedia and interactive content. Also the possibility of editing the content and sharing resulting changes with other students is a key attribute. The dissertation then moves on to describe the different kinds of study materials that are in use at VSE. The second half of the dissertation builds on previous analysis of the study materials and introduces a hypothetical optimal state, which could result from implementing e-book technology. This state is the answers the question of what is actually possible to achieve. The next chapter in this half is the technical analysis, which answers the question of how is it possible to achieve the suggested hypothetical state. The conclusion of this dissertation answers the questions whether the proposed solution is a viable candidate for implementation. The results of this dissertation could be used as a guideline for the project of implementation of e-book technology in the environment of VSE.
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Construction of a triangle - interactive aid
KRATOCHVÍL, Daniel
This dissertation informs readers about self-made interactive aid, enabling to understand the problematic of the construction of the triangle. The basic information about this aid, it´s preference and usage is illustrated on some several cases of the solution to tasks of the construction of the triangle. The interactive aid is appropriate for teaching, but also for consesutive usage about the solution of concrete tasks.
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