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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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Chosen natural resources: focuses of conflicts nad its economic consequences
Sytný, Patrik ; Vošta, Milan (advisor) ; Sankot, Ondřej (referee)
The bachelor´s thesis aims to three chosen natural resources, which are petroleum, natural gas and water, and conflicts based on disputes over them. The aim of the thesis was to valorize nature of the conflicts, define their main focus and draw prospective economic consequences. Professional literature that deals with this kind of issue, was used in order to find out the true nature of the conflicts. The result is an evaluation, which has proven, that the most influental conflicts are rising over disputes over petroleum. Their economic and political impacts are very perceptible and economically measurable in the way of GDP, the total product and other economic indicators. In the thesis has been also proven that the conflicts on the basis of natural gas have similiar nature and extent as it is in the case of petroleum, even thought in a smaller amount. The least important conflicts in a meaning of economic impacts are those over water, by those has been proven, that they do not poses such an impact on economic and political situation as petroleum or natural gas nowdays.
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Analysis of the Motivation and Satisfaction of Employees in a firm
Gonta, Natalya ; Dvořáková, Zuzana (advisor) ; Odvárková, Markéta (referee)
The aim of this study is to analyze the motivation and satisfaction of employees at British American Tobacco Ltd. The bachelor thesis consists of two parts - theoretical and practical. The first part describes the concepts and sources of motivation, which affect the performance of employees in the company. The second part describes the aim of the research, its progress and evaluation results in respect of which was carried out to analyze the way of motivation and satisfaction of employees in the company. For the following analysis methods were used questionnaire and a personal unstructured interview. The result of the thesis is mentioned proposal for improving the motivation of employees, which has potential and could be implemented in the company.
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World Trade in Crude Oil
Benešová, Nela ; Čajka, Radek (advisor) ; Bolotov, Ilya (referee)
This master´s thesis deals with trade with crude oil and its impact on the world economy. Everyone, who wants to start to trade with crude oil, should find out basic terminology and information about crude oil that is characterized in the first chapter. The hunt for oil started many years ago, therefore the history is also mentioned in this master´s thesis. The second chapter focuses on the world trade with crude oil. Territorial structure and trade flows are described in this part of the master´s thesis. Nevertheless, the crude oil also has an impact on the world economy and economies of single states. The third chapter analyses states, where the inhabitants live beyond means of people from other countries but these states are so depended on crude oil that if this trade stopped, these countries would go bankrupt. However, there are also exceptions. Even though, there are states that have huge sources of crude oil, they still have to deal with financial problems. The forth chapter characterizes the trade with crude oil of the Czech Republic because it is a net importer of crude oil and the last chapter focuses on new trends in crude oil such as shale oil and trading at a crude oil market.
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Corporate social responsibility of SAP
Vaníková, Zuzana ; Müllerová, Františka (advisor) ; Sitter, Barbora (referee)
The thesis deals with corporate social responsibility. The subject of investigation is the CSR strategy of the chosen company. The theoretical part describes the corporate social responsibility from a historical and a current perspective. Above all, the thesis puts emphasis on the social pillar, namely the activities of the company in relation to its stakeholders, particularly employees and volunteering. Volunteering is an important part of corporate social responsibility. The questionnaire and subsequently its statistical evaluation answers the question whether the students currently studying in two economic universities consider SAP as a responsible company and how they relate to volunteerism.
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The Conclusion of a Contract under the United Nations Convention on Contracts for the International Sale of Goods in Comparison with Czech National Legislation
Krčálová, Kristýna ; Boháček, Martin (advisor) ; Levý, Jan (referee)
This Master's thesis focuses on the United Nations Convention on Contracts for the International Sale of Goods (CISG), better known as the Vienna Convention. The main purpose of this thesis is to evaluate conditions for application of this international treaty and to assess the contracting process under the CISG including the similarities and differences with the Czech national legislation. Finally, it seeks an answer to the question, which of these rules (international or national) are more advantageous for a Czech contractual party with respect to concluding a contract. The thesis is divided into five parts. The first one represents an introduction to the CISG, its origin and the Czech Republic's attitude to it. The second part identifies the conditions for application of the CISG and therefore defines a subject and an object of an international contract of sale under the CISG. Both third and fourth parts gradually introduce two phases of the contracting process (offer and acceptance) including the relevant case-law. The last chapter strives to complete the contracting process with an analysis of the exact moment, when a contract is concluded, as well as with an analysis of a potential battle of the forms and its impact on a contract conclusion.
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The current level of compliance with the concept of prenatal care from the perspective of pregnant women
URBÁNKOVÁ, Martina
This bachelor thesis deals with a current level of abidance of the prenatal care conception from the point of view of pregnant women. Theoretical part is divided into four main chapters. The first chapter is focused on the history of midwifery and prenatal care. The second one describes prenatal care in general, organisations, goals, antenatal preparation by the midwife, pregnancy card and a role of the midwife in the prenatal consulting. The third chapter deals with pregnancy diagnosis and the last one concerns the examination of pregnant women in each trimester. Practical part of the thesis points out a comparison of a current level of abiding the prenatal care conception in České Budějovice and Benešov region.Three goals were set prior to writting this thesis. The first goal was to find out the abidance of the prenatal care conception from the point of view of pregnant women. The second goal was to find out, whether pregnant women understand the importance of all the examinations carried out during prenatal consultings. The third goal was to find our and compare the level of abiding the prenatal care conception in České Budějovice and Benešov region. Three questions arose from these three goals: What is the level of abiding the prenatal care conception? What examinations are being carried out during prenatal consultings? What is the difference between abiding the prenatal care conception in České Budějovice and Benešov region?The qualitative method had been used to conduct the survey.Answers to the questions from the survey were searched during the acquisition and processing of the semi-structured interviews. Conclusion of the survey is, that all the pregnant women are satisfied with the current conception of the prenatal care. This way of timing of visit intervals in prenatal consultings seems right to them, important and necessary, not only for a control over themselves, but also for a control over the fetus. The level of the conception of prenatal care is good, satisfactory for all the mothers. Surprising result was, that half of the mothers is unable to describe and elaborate what examinations they went through in prenatal consulting, when it was and what was its purpose. Especially when only two respondents complained about insufficient awareness from the physician about performed examinations. As the most common examinations were stated: blood pressure, urine, weight, CTG scan and vaginal examinations. Based on the conducted survey I have managed to find out that the frequency of regular visits in prenatal consulting, according to CGOS recommendations in České Budějovice was abode only in two cases. This fact was not confirmed by any respondent from the Benešov region. Schedule of the regular visits depends on physician´s consideration. Another difference was not discovered. Current level of abidance of the prenatal care conception in Benešov region is similar to České Budějovice.The thesis may serve as an informational material in prenatal consultings in the future, especially for pregnant women, who want to know the progress of prenatal care.
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Comparison of old and current cine lenses in combination with modern digital camera
Jakubec, František ; HOJDA, Petr (advisor) ; MIKŠ, Antonín (referee)
A Director of Photography has many artistic possibilities when working with cine lenses. Today, we can produce many optical effects in digital post-productions; however, working with lenses and with their defects (aberrations) is very interesting when you use them with modern recording technology. This thesis examines a small part of these issues. The theoretical part is about general qualities of optical systems, their aberrations, and measurement of picture quality. Furthermore, there is also a description of the movie camera and lenses, which were used for testing. The analytical part is about analyzing real footage from tests.
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The Role of a Nurse in Care of a Patient with Mononucleosis
LAŽANSKÁ, Soňa
Theoretical background Infectious mononucleosis belongs to relatively widespread viral infectious diseases in the Czech Republic. Epstein-Baar virus (EBV) participates in occurrence of the infection by 80 %, infectious mononucleosis may more rarely be caused by cytomegalovirus. Each individual factually suffers from infection caused by EBV during his/her life, however its course is unapparent in most cases. An affected individual or an individual not affected, who is a virus carrier may be considered the source of the infection. Infectious mononucleosis usually affects children and teenagers, in older individuals the infection is quite rare. The disease might start rapidly or gradually with prodromes like headache, sore throat, nausea, fatigue and perspiration. Infectious mononucleosis is often confused with other diseases like e.g. tonsillitis, or more serious progress of flue, particularly for the symptoms accompanying the disease. Fever, lymphadenopathy and hepatosplenomegaly are the most frequent symptoms. The disease may also cause numerous unpleasant complications, particularly hematologic, neurologic, cardio-pulmonary and autoimmunity complications. A nurse plays a very substantial role in client care. It is very important for a nurse to have sufficient knowledge and to know the right procedure of all interventions he/she meets within diagnostics and treatment of infectious mononucleosis. These are particularly biological material collection, per os administration or intravenous administration of medicines. A nurse should also be familiar with the Priessnitz compress application technique. In non-self-sufficient clients a nurse is also responsible for client's hygiene. A nurse has an important role in client education providing information on dietetic measures. A nurse has to lead a client to adherence to the prescribed diet and body calm. Everything depends on client's current condition. A client should also avoid mental strain during convalescence. We should note that not all forms of infectious mononucleosis require hospitalization. The aim of the thesis The aim of the thesis was to find out the role of nurses in the care of patients with infectious mononucleosis and also to find how satisfied patients with the nursing care and education are after going through mononucleosis. This was why the following research questions were chosen: What is the role of a nurse in care of a patient with mononucleosis? How satisfied are patients with the nursing care after going through mononucleosis? How satisfied are patients with education in diet regime adherence after going through mononucleosis? Applied Methods Qualitative research was chosen for finding out the required information. The information was gained by means of deep interviews with nurses working at the infectious department of České Budějovice Hospital and with clients after going through infectious mononucleosis. All the interviews were recorded on a voice recorder and then transcribed. The transcribed interviews were subject to detailed analysis by the paper and pencil method with open coding. Results Six categories were set upon analysis of the interviews with nurses: Awareness of the infectious mononucleosis disease among nurses, Nursing care of a client with infectious mononucleosis, Regime measures, Information on dietetic measures, Recommendations after infectious mononucleosis and Education of a client with infectious mononucleosis. Five categories were set upon detailed analysis of the interviews with the clients: Recommended treatment, Nursing care, Recommended measures, Methods of information communication and Information sources for clients. Subcategories, in which the key data are coded, are assigned to the individual categories.
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Nursing care of the operation area before and after the operation
KRATOCHVÍLOVÁ, Michaela
Theoretical outcomes The preparation of the operating field is an important part of all preoperative preparation. Preoperative preparation, before surgical intervention, cannot be neglected. In most cases, the doctor will not perform an operation without proper preoperative preparation. The only exception is urgent surgical intervention for which only the most basic preoperative preparation is provided. Preoperative preparation can be categorized as long-term, short-term, or immediate. A thorough and proper preparation of the operating field reduces any development of postoperative wound complications. The aim of thorough preoperative preparation of the operating field is to reduce any risk of developing postoperative wound complications. Postoperative care prevents postoperative complications. It is aimed at controlling the state of a patient's current health after surgical intervention, but also the surgical wound and its dressing. Both nurses and doctors are obliged to perform these controls. In the early postoperative period, the client stays in the ward or in the postoperative room. In the case of arising complications during surgical intervention, the client is moved to the ICU/ARO. The Aims of the Thesis The aim of the thesis is to map the differences in preoperative preparation of the operating field among surgery departments, and to find the differences between preparation in the operating field before a scheduled and an urgent surgical intervention. Furthermore, the aim is to find out if nurses perform preoperative and postoperative care properly. The following questions were chosen: What are the differences in preoperative preparation of the operating field among surgery departments? How do nurses perform preoperative and postoperative care of the operating field? Implemented methods In order to gain the desired information, a qualitative form of research was chosen, namely an in-depth interview method, a method of questioning, and an observational method. Observation was implemented using the hidden observation method. The respondents in the research were the nurses from the urology and the surgery departments at a hospital in Vysočina. Data collection was conducted from February to March, 2014. The acquired data was then analyzed through an open coding method. Results The subsequently transcribed interviews and observations were carefully analyzed using the 'pencil & paper' method and open coding. Eventually, five categories were created: preoperative preparation before a scheduled surgical intervention, preoperative preparation before an acute or urgent surgical intervention, preparation of the operating field, postoperative wound care, medical documentation. Conclusion The research suggests that nurses are well aware of the complete preoperative and postoperative care. What is, however, surprising is how adherence to the principles of barrier care and the client's intimity vary in the course of providing this care.The conclusions in this thesis should aim at an improvement of preoperative and postoperative care of a client and to eliminate any discovered shortcomings in the provided nursing care. The practical outcome of this thesis will be an article in a professional magazine for nurses.
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