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Expression of selected proteins in sperm in men with normal and pathological spermiograms using monoclonal antibodies
Pěknicová, Jana ; Čapková, Jana ; Dorosh, Andriy ; Margaryan, Hasmik ; Kubátová, Alena ; Děd, Lukáš
Recent studies show that infertility in human populations it affects an estimated 15% of couples of reproductive age. Male infertility is the primary cause for 60% of these cases. For these reasons, we analyzed the acrosomal and sperm surface proteins in men with normal and pathological spermiograms. We found that intra-acrosome proteins: TERA (Transitional endoplasmic reticulum ATPase), GAPDHS (Sperm Glyceraldehyde-3-phosphate dehydrogenase), and PRKAR2A (C-AMP-dependent protein kinase II, PRKAR2A), which can be identified using our monoclonal antibodies, are different express in healthy men and men with astenozoospermia (with reduced sperm motility), and with a significantly reduced expression in the astenozoospermia. These proteins are involved in energy metabolism and apoptosis of the cells, and some of them in the sperm-egg interaction; therefore, they have an important role in reproduction. On the other hand, there were no statistically significant differences in the expression of surface proteins (Appolipoprotein J (Clusterin) and Semenogelin). Our findings show that astenozoospermia as a complex disorder of the semen is often combined with other pathological conditions that are not diagnosed by the semen analysis. Therefore monoclonal antibodies are so suitable instrument for the detection of proteins associated with the pathology of the sperm in the semen with low sperm motility. In general, monoclonal antibodies against the sperm proteins are an appropriate tool to detect sperm quality in reproductive medicine.
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Differentiation of offers and methods of working physiotherapy of persons with a mental or combined handicap in a practice of social service providers and another subjects.
POŠMUROVÁ, Nikola
In the Bachelor's thesis I have dealt with differentiation of tenders and rehabilitation methods. Specifically, individuals with mental or multiple disabilities and for providers of social services and other bodies. In the theoretical part I focused on defining the basic terms relating to that issue. In the introductory chapter I dealt with the definition of the concept of mental disability, causes of its creation and definition of degrees of mental retardation. The next chapter is devoted to the combined disability, specifically the yoke of the definition, causes of and classification. In the third chapter is devoted to the importance of the work, the status of the work in a person's life and the status of work for persons with disabilities, including individuals with mental or multiple disabilities in Bachelor thesis I. In the next chapter, I deal with comprehensive rehabilitation system, specifically the remedies rehabilitation, educational resources, social resources, rehabilitation, rehabilitation and special attention devoted work resources, rehabilitation, which will directly affect the Bachelor thesis. Furthermore, I determine a target group the work of rehabilitation and the preconditions for its implementation. In other chapters is devoted to the specification of tools and resources work rehabilitation, employment policy tools and I define jobs that are offered to persons with intellectual disabilities. In the practical part of the thesis, first determine the main aim and target work. The main goal was to determine what are the menus and methods of rehabilitation for people with mental or multiple disabilities with providers of social services. As the target I had set to determine what are the possibilities of transmission from individual to social services on the open employment market. Specifically, I've focused on the resources provided by the rehabilitation of daily, weekly care centers or day service center, in addition to the socio-therapeutic workshops, in a sheltered employment, and employment on the open employment market. To meet the objectives I used a qualitative approach, specifically the semi-structured interview with six providers of social services of the South Bohemian region The analysis of the information obtained, I discovered that if the device is operated daily, weekly social welfare institution or Centre, day services, the services provided are identical, not different from each other. In the case of socio-therapeutic workshops is the diversity of the wider, each device provides a variety of services, different options, some devices have socio-therapeutic workshops directly on the device, others have socio-therapeutic workshops and externally, outside of your device. Protected jobs are also in the majority of providers of social services offered both within and outside the device, always the odd jobs, such as in the kitchen, cleaning, in teahouses and the like. It always depends on the type and degree of disability, on the basis of a levy for the client to the appropriate activities. Location of the client on the open employment market is not very common in most devices, some devices that offer clients or do not provide. Partial objective was to detect transmission, I found that the only limiting factor is the client's disability that limits its options and device options, of course, that the services it offers and delivers. The aim of all devices is to achieve the greatest possible degree of autonomy of the client and allow it to advance from care centers or day service center in the socio-therapeutic workshops, found a protected job, and a job on the open employment market.
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The need for security and safety in connection with a planned surgery with the patient's perspective and nurses
BOHDALOVÁ, Jaroslava
A planned operation performance always affects patient´s needs for assurance and safety. It is important to provide necessary information connected with the planned operation performance itself so that the patient can feel assure and safe. The key role for the patient and her/his needs for assurance and safety is in general nurse´s hands because she is the closest person to the patient and can help to inspire her/his needs for assurance and safety with konfidence best through the aid of her knowledge and skills that she should have as well as through the aid of her attitudes. The target of the thesis was to find out how a planned operation performance affects patient´s needs for assurance and safety and how a general nurse inspires patient´s needs for assurance and safety connected with a planned operation performance with confidence. The following research questions were stated: How does a planned operation performance affect patient´s needs for assurance and safety? How does a general nurse affect patient´s needs for assurance and safety connected with a planned operation performance to inspire her/him with confidence? What is insufficient in filling patient´s needs for assurance and safety before a planned operation performance? How can a general nurse help a patient control her/his fear and state of anxiety related to a planned operation performance? The method qualitative research with the use of depth interview was used to figure out the investigation. The respondents were willing to record the interview which were verbally rewritten and then subjected to the contents analysis by the method paper and pencil. From the research it is clear that a planned operation performance affects patient´s needs for assurance and safety in a negative way disregarding to her/his previous experience. Patient´s worries and negative feelings can be eliminated by a general nurse who treats her/him as equal, taking up an individual approach, informing her/him about all performances and proceedings, having a calming and welcoming way of communication, using empathy and listening as well as trying to meet patient´s wishes according to her possibilities. The research reveals the advantage of patients´ readiness for planned operation performances. Patients can learn it in the framework of educational proceeding in the out-patients´ department at the same time when they learn that they are going to be operated. The research also shows that general nurses do not have enough knowledge in the sphere of therapeutical communication. The results indicate that it would be suitable to start educating patients as soon as it is known how their disease will be solved and treated in order to reduce the negative impact of surgical treatment to patients´ needs for assurance and safety. General nurses should complete their knowledge and skills to be able to help patients to cope with their difficult life-situation. On the basis of the research investigation and after the consultation with the senior nursing officer, the training course will be organized. The topic of the training course will concentrate on communication with patients before planned operation performances with a view to therapeutical communications and therapeutical interviews. Other discovered facts, for example, the lack of information provided to patients and possibilities regarding better patients´ readiness for planned operation performances will surely be solved by the hospital management.
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Pick´s disease and the specifics of the nursing care of a patient with this disease
KOPKAŠOVÁ, Lenka
Pick's disease is a degenerative-atrophic process that primarily affects the frontal and temporal lobes of the brain. Besides Alzheimer's disease, it is a lesser known form of dementia which affects people of younger age. Although the exact range of Pick's disease occurrence varies in the literature, the average reported age is between 50-60 years. The disease can change eating habits, cause emotional swings, inappropriate behaviour and sloppy appearance of the patients. The behaviour to others is often selfish, the patients are unable to listen to others and ignore their surroundings. Pick's disease is also characterized by inappropriate sexual behaviour. Globally, the number of dementia patients is still growing and according to expert guesses the increase will continue. Nursing care for patients with dementia is therefore becoming a very actual topic as dementia is called a quiet epidemic of our century. Nurses in their departments quite frequently encounter patients suffering from dementia of various origins at different stages of disability and the staff at healthcare facilities often regard these people as a burden. The aim of this thesis, which is called Pick's Disease and Specifics of Nursing Care for Patients with this Disease, is, based on the theoretical knowledge, to map definitions and diagnosis of Pick's disease, to define the needs of such a patient, and to list the most common problems in the nursing care. It focuses on people with dementia within medical facilities. For proper understanding of the problem, the thesis starts with the anatomy and physiology of the brain and the description of cognitive functions which are affected by the dementia syndrome and the definition of dementia. Based on the studied literature, dementia is divided according to various criteria. It has been found out that dividing dementia is not entirely uniform, but the essential facts and overall opinions of the authors do not diverge. Pick's disease belongs to a group of frontotemporal dementia and its position in this group has changed over time, as it is recorded in the literature. The thesis includes recommendations resulting from the studied literature in the approach to patients with dementia and emphasizes personal communication with the patients and empathetic and humane approach. Although medicine cannot prevent dementia, an early diagnosis of a specific type of dementia and correctly targeted treatment can increase the quality of life not only of the patients but also of their families. I have chosen this topic because I find it very current and interesting. During my nursing practice at the Surgery Department and Aftercare Department, I have met patients suffering from dementia and I did not know how to properly and efficiently communicate with them. For the theoretical part of my work, the methods of explanation, synthesis and induction on the basis of Czech and foreign information sources have been used. The most frequently quoted Czech authors in my thesis are doc. MD. Roman Jirák, PhD., the head of the center for diagnosis and therapy of Alzheimer's disease at the psychiatric clinic of the General University Hospital in Prague, the 1st Medical Faculty of Charles University. This thesis can serve as an informational and educational source both for nurses and the general public who may experience the disease within their families. It would be considered a great success if it helped nurses or family members to diagnose Pick's disease or other dementia at an early stage.
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Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.
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Ability of dogs to discriminate human odor exposed to chemical detergent
Čajágiová, Martina ; Vyplelová, Petra (advisor) ; Jaroslav, Jaroslav (referee)
In the recherche part of our thesis we familiarize ourselves with the human odor, theories of its origin, definition, anatomy of human skin and odors, composition of individual human odors and with odor secretion. We also look closer on the topics of odor absorbent and its use in the world, the transmission of odors to odor sensors and securing of scents. This section of our thesis discusses the resistance and survival abilities of odor, scent identification method and its history, organic acids, and defines the application of laundry detergents and ultrasonic washers. Aim of our thesis was to verify the relevance of the use of chemical detergents in the purification process when working with scents. We tried to verify the ability of detergents to degrade the human scent on odor absorbents to such an extent, that specially trained dogs would not be able to identify it. Our experiment was following a precise determination methodology. In the first phase samples were collected from the hands of targeted persons to a scent carrier - a metal cylinder (extirpate odour), by one researcher. In the second phase samples were collected from the body to a textile carrier, by another researcher. The metal scent carriers were processed. Some of them were left as they were (control sample) and some were exposed to chemical detergents with and without usage of an ultrasonic washer.
In the experiment where we tried to identify the odour samples, six bitches of German shepherd were used. They were specially trained for odour identification. Each dog was let three times to identify the target scent exposed to a detergent and three times to identify the target scent not exposed to a detergent. Target smell was randomly deposited between other samples and its position was changed, so that the handler did not know its position and thus was unable to affect the work of his dog. The indicator of positive identification was a sign the dogs were taught - to sit or lay in front of a sample.
Any dog was unable to identify the scent which was exposed to chemical detergent and all dogs identify the scent unexposed to a chemical detergent. Our experiment has shown that the use of chemical detergents in the purification process when working with scents is relevant to the degradation of individual human scent.
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Appaloosa breeding and their utilization
Zuzjaková, Eva ; Neumann, Cyril (advisor) ; Starostová, Lucie (referee)
This bachelor thesis is a summary of the basic information about breeding and utilization of appaloosa. A dominant characteristic of appaloosa is an attractive color coat pattern, which is caused through a set of genes called Leopard Complex (Lp). That is the reason, why in this thesis, at first, the history of presence of the Lp in genus Equus takes place. The presence of Lp can be found already in many cave paintings of prehistoric horses. During domestication, the coat pattern became important. The horses of the same color coat pattern, which have today appaloosa, are depicted in the Celts, Scythians and Etruscans tombs, as well as in murals of Chinese and Egyptian cultures. Significant breeding of the spotted horses are also documented in the Ferghana Valley in eastern Uzbekistan. In Europe at 16th century horses were expanded and a lot of them have spotted coat pattern. Then the Spanish settlers imported these horses to the America, where Nez Perce Indian tribe bred horses, which we know today as Appaloosa.
Furthermore, in this thesis the Appaloosa breed organization, which belongs complete to Appaloosa Horse club of America, is described. The breeding standard and characteristics, which are color coat pattern, mottled skin, visible white sclera and striped hooves are inducted. Seven various types of coat pattern and tree types of registration are also described.
The major problems in the appaloosa breed are inherited diseases. In this thesis the most serious five of them are identified. It is CSNB, congenital stationary night blindnes associated with a homozygous variant of LP gene. HYPP, hyperkalaemic periodic paralysis, which causes muscle spasm of affected horses. This disease was obtained from quarter horses. HERDA - Hereditary Equine Regional Dermal Asthenia which causes irreversible damage to exposed skin. Another serious disease is PSSM, polysaccharide storage myopathy, which is one of many myopathy occuring in horses. The latter disease is lethal GBED. Affected foals lack the enzyme GBE which is necessary for the synthesis and storage of glycogen. In spite of the improving precautions, these diseases have not been fully eliminated from breeding.
Further, there are given the possibilities of using Appaloosa horses in sports, hobby and ranch works. The system of shows and their ratings is described as well.
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The effect of the operator on the accuracy of the estimates of lean meat share in pigs
Jiravová, Renata ; Šprysl, Michal (advisor) ; Libor , Libor (referee)
The aim of the study followed the determination of operator error, ie repeatability, or influence of the measuring point on the accuracy of the estimate of lean meat share (LMP). It was a FOM-SFK instrument measured at prescribed locations the backfat thickness, muscle depth, and thus their LMP in their carcass realization in SEUROP system. For this purpose a total of 71 hybrid pigs (Dan-Bred) were measured at the Velvary slaughterhouse. In order to determine the error from incorrectly determined place of measurement, the following six classification insertions were done per one animal, thus 1. 2nd -- 3rd last rib 7 cm off the midline (right point), 2. 2nd -- 3rd last rib 7 cm off the midline (repeat in the same hole), 3. 2nd -- 3rd last rib 1 cm caudal to the right point, 4. 2nd -- 3rd last rib 1 cm cranial to the right point, 5. 3rd -- 4th last rib 1 cm medial to the right point, 6. 1st --2nd last rib 1 cm lateral to the right point. Measurements were performed on the carcass that insertion 2 should be identical with insertion 1, insertions 3 and 4 were moved 1 cm off insertion 1 in the cranial or caudal direction and insertions 5 and 6 were moved by 1 rib from insertion 1 in the cranial or caudal direction respectively. Classification was held by FOM instrument, for further comparison also by ZP method. For the above classifications following regression equations were used. For: - FOM y= 81,8909+0,2006*M+14,1911*ln S, kde M=MLLT depth, S =backfat thickness, - ZP y= 76,6722--1,0485*M+0,00794*M2--0,002884*S2+9,0151*ln (M/S), kde M = MLLT depth, S= backfat thickness. Calculation and comparison of the results was performed by statistical program SAS Propriety Software Release 6.04, differences were tested by analysis of variance. Based on the results we can say that a given hypothesis was confirmed. Also, in the realization of slaughter pigs in SEUROP system can be stated that - by invasive technique FOM - precise LMP determination of the carcass is a function of the need for precise determination of the puncture site of operator, - accuracy of the backfat thickness as well as LMP estimate is affected by repeated punctures, - more reliably is measured the backfat thickness with repeated injection than the muscle depth due to its possible deformation, - inaccuracy of repeated measurements (repeatability) will not significantly affect the carcass classification into classes at the slaughterhouse; any errors will occur to the detriment of the supplier, - the injection shift 1 rib cranially respectively caudally affect the overall carcass classification more than shift the injection 1 cm medially or laterally away from the spine, - in the case of an unsuccessful measurement the best is to repeat the puncture or the second puncture shift 1 cm caudal or repeat puncture between the 2nd and the 3rd thoracic vertebra 1 cm towards the spine, - non-invasive technique ZP, compared to FOM, underestimates the LMP estimate by about 1,4%.
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Use of Festuca arundinacea for decorative lawns
Doskočil, Jan ; Svobodová, Miluše (advisor) ; Hrevušová, Zuzana (referee)
Use of Festuca arundinacea for decorative lawns
Summary
Festuca arundinacea is briefly bentgrass with broader and coarser leaves. Hardly perennial grass, native to Europe, is well known for its high resistance of dry weather, low intensity of maintenance and resistance of high use. These qualities gains thanks to its well-developed and deep root system, which is capable to gain water and nutrients from deeper parts of soil. Its use is in places with high use, like a race-course, edges of roads or vineyards alleyway. Today, in time of global warming, its usage becomes more important for park purposes and low-input turfs, where is its higher resistance against biotic and abiotic stresses, mainly against drought, utilized. Festuca arundinacea complement well with Poa pratensis in turf.
Goal of this work was to evaluate turfs with Festuca arundinacea and chosen grass species under different mowing frequency. According to hypothesis frequency shouldn´t affect turf coverage. Coverage should be same with different mixture composition with Festuca arundinacea.
The experiment was performed on experimental land of Czech university of Life Sciences Prague, Suchdol in 2015. Mixtures of Festuca arundinacea with Festuca rubra, Poa pratensis and Lolium perenne were seeded together with monocultures of these species in 2012. Mixture composition was 50/50 except mixture of Fesruca arundinacea and Poa pratensis, where the mixture was 75/25. There were conducted 63 plots, in 3 repetitions and in 3 different frequencies of mowing (by 14, 30 and 45 days). Each plot was 6 m2 big. The results were evaluated by analysis of variance ANOVA (P is smaller than 0.05) in program Statgraphics, version XV.
Influence of mixture composition to level of growth was significant. The highest level of growth has monoculture of Festuca arundinacea (10,6 cm). The lowest level of growth has monoculture of Lolium perenne (5,4 cm). Monoculture of Festuca arundinacea had the highest intensity of grow (0,07 cm/day) at 14-day frequency of moving. Lolium perenne has the lowest intensity of grow (0,02 cm/day). At 30-day frequency of mowing mixture of Festuca arundinacea with Poa pratensis has the highest intensity of grow (0,24 cm/day). The lowest intensity of grow has Lolium perenne (0,01 cm/day). The highest intensity of grow was at 45-day mowing frequency. The highest intensity of grow has mixture of Festuca arundinacea with Festuca rubra (0,75 cm/day). The lowest intensity of grow has monoculture of Lolium perenne (0,06 cm/day). Influence of mixture composition to coverage was statistically significant. The highest coverage has mixture of Festuca arundinace with Festuca rubra (81,3 %). The lowest coverage has monoculture of Lolium perenne (58,6%). Influence of frequency to level of growth was statistically significant. The highest level of growth has 45-day frequency of mowing (6 cm) and the lowest level of growth has 14-day frequency of mowing (3,8 cm). Influence of frequency to coverage was statistically significant. The highest coverage 14-day frequency of moving (76,5 %) and the lowest has 45-day frequency of growing (66,7 %).
The knowledge gained will be used for further compilation grass mixtures for park lawns. It turned out that it is better to prefer seeding mixtures with Festuca arundinacea than monocultures. To achieve high coverage and optimal intensity of growth with Festuca arundinacea was recommended to follow 30-day frequency of mowing.
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Factors affecting postmortem changes in pork meat
Seilerová, Michaela ; Čítek, Jaroslav (advisor) ; Lebedová, Nicole (referee)
The thesis focused on the summary of the quality of pork. The main indicators of the quality of pork and of meat generally together with a chemical composition and the external and internal factors dealing with the quality of meat are described in the thesis. The various histological structure, the variable chemical content, technological features are found in meat. The structure and composition depends on a way of living, the function of the parts of an animal body, on lots of intravital factors (a kind of an animal, breed, male or female, age, the way of nutrition, the state of health). Meat is a valuable source of nutrients and essential acids for a man. The human organism is not able to synthesize the essential acids itself. Lipids are the important part of meat, their main function is storing energy. They are found as a reserve tissue in the subcutaneus parts of animals or among muscular fibre. The amount of carbohydrates contained in meat is not important, meat is low in carbohydrates. They are only important for meat maturation. Meat is high in minerals and vitamins. It is necesary for a man and should be a part of our diet. The important factor for a meat quality is described as a formation of muscular fibres. There are the thin fibres in the meat of a high quality and the thick and fat fibres in the meat of a low quality. The meat quality depends on a number and charactes of animal fibres. The meat of a high quality is for example loin - it is the meat with fine fibres. The next factor which has influence on a meat quality is course of post mortem changes.We can differentiate four stages in these processes: prae rigor, rigor mortis, aging of the meat and deep autolysis. Post mortem aiging processes can deviate from teh standard from variety of reasons and as concequences we can see quality deviation of the meat for example PSE, DFD, RSE, Hampshire efect and cold shortening.Also the breed has the influance on the quality of the meat namely sensitivity to stress,growth characteristic and parameters of slaughter value, other internal factors are age and sex. External factors which has the influance on the quality of the meat are transport of the animals to the slaughterhouse, attitude of the employees during chasing after the animals, the rest before slaughter, stunning itself,bleeding thereafter and cooling down after slaughtery.
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