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Comparison of methods of ECLIA and ELISA in determining the beta-CrossLaps
KOŽANTOVÁ, Jana
Osteoporosis is a very old disease affecting a large majority of population. Its occurrence rate has been growing permanently. It is a systemic disease of bones for which the decrease in bone mass, i.e. of inorganic as well as organic bone part is characteristic. Typical symptoms are osteoporotic fractures. To perform an in time diagnostics of the disease, it is necessary to execute the necessary examinations. One of them is also the laboratory determination of markers of bone resorption and new creation for finding out the state of bone metabolism. The target of my bachelor thesis was to find out the level of the bone resorption marker (beta-CrossLaps) by means of ELISA and ECLIA methods on the same serum from patients with the diagnosed osteoporosis and to process statistically the results. In the next part I dealt with the structure and functions of bones. Then I focused on markers of bone formation and resorption. Finally I mentioned the bone metabolism and its control mechanisms. I concentrated mainly on the metabolism of calcium, D vitamin, parathormone and calcitonin. I carried out the practical part of my bachelor's thesis in the accredited private laboratory STAFILA, spol. s r. o. in České Budějovice. The procedure of my work followed the standard operating procedure of the laboratory. In the research, in total 66 serums of patients with osteoporosis were analysed by me. At first, I determined the concentration by beta-CrossLaps method based on the principle of sandwich ELISA on the appliance NexGen Four from TestLine company. Then I analysed the same samples on the automatic analyser IDS-iSYS from IDS company, this time by ECLIA method. Both appliances on which the determination was executed, principles of both methods, applied analytic sets and the procedures of analysis are described in the methodical part. In the next part of my bachelor's thesis, the results of my measuring are stated. At first I entered the concentrations beta-CrossLaps in ng/ml measured by both methods into the table and for the respective samples I stated the sex and years of birth of individual patients. Moreover I prepared a graph illustrating the share of men and women of various age categories in my research. The second graph shows the differences in values of beta-CrossLaps concentrations between both methods. Resulting concentrations were subject to regression analysis. I applied linear regression and Grubbs test for finding out the outlying values excluded from the further statistical processing. I performed Passing-Bablok regression based on the remaining values. As a result of regression equation and 95% CI of the slope B and intercept A, the proportional error of measuring was rejected but on the contrary, the systematic error was confirmed. Correlation coefficient R = 0,97 shows a very good linear dependency between both methods. It turned out by practice in the laboratory that the evaluation of concentrations beta-CrossLaps from the same patients on various appliances may show partially different values. I worked at two different analysers, each of them was based on another principle, with a different sensitivity and calibration. All of this could have the influence on the resulting concentration of individual samples. The advantage of automatic analyser IDS-iSYS is a quicker determination. Moreover it is possible to perform on this apparatus all the possible examination concerning the bone metabolism from D vitamin, parathormone, osteocalcin up to bone markers. It is advantage for the patients with osteoporosis as well as with other metabolic skeleton diseases. It follows from this that all the necessary examinations may be carried out on one apparatus.
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Podnikatelský plán vybrané firmy (model lean canvas)
Strupplová, Lucie ; Filipová, Alena (advisor) ; Zeman, Jiří (referee)
The well arranged and instructivee business plan is the basic part of every business project.. Currently is very often used the lean canvas model of the business plan. This model shows us very istructively all the factors influencing the project realisation. The dissertation is divided into the two basic parts. In the thoretical were compared the essential forms of the business plan with their differences. The practical part consists of creating the lean canvas model for the progressive company using the UAV and the MAIA application, monitoring the flight of the UAV. The economic data were analyzed and the structured scheme of the project, with the prediction was realized. The lean canvas model was very useful and optimal for this company and it´s project.
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Výběr a implementace open source nástroje pro řízení portfolia projektů
Marek, Jan ; Chlapek, Dušan (advisor) ; Kučera, Jan (referee)
Methods and ways of implementation of changes and innovations in companies through project management are in today's society very well established. There exists methodologies, techniques and tools for the management of individual projects. However in a role of project manager, I very often faced the fact that companies are performing the project portfolio management in very intuitive way. This in itself leads to failed and prematurely terminated projects, initiation of the wrong projects or realisation of correct projects, but at the wrong time. Very often I have also recognized, that there is lack of awareness of fact, that there are also Open Source applications that can help with the organization's portfolio. This thesis deals with the definition of requirements, search and selection of proper OSS application and subsequent implementation. In the first part of this thesis I prepare a theoretical framework about PPM, and on that basis then identify and verify a set of requirements for selection of right OSS PPM application. The next section of thesis describes searching for suitable applications from a variety of sources, assessment against the requirements and the final selection. In the last part the implementation project is drafted, which aims to serve other colleagues in project management as one of the possible implementation paths. The outputs are then continuously confronted with experts in matter of projects and project management in the field of IT in order to maximize symbiosis between theory and real life experiences. Virtually every project management methodology defines the collection of already-proven techniques, best practices or lessons learned from the previous similar implementations. The main contribution of this thesis I see in fact that it contains not only the design of the project implementation, but also describes a logical path, what leads to the result. Therefore this thesis could be used as a base or discussed best practice, when a project of implementation of PPM application in place.
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Access to Information and Access to Justice in Environmental Matters in the Republic of Irealand and the Czech Republic
Vítková, Martina ; Stejskal, Vojtěch (advisor) ; Sobotka, Michal (referee)
This diploma thesis deals with access to environmental information and access to justice in environmental matters introduced by Aarhus Convention in the specific conditions of Czech Republic and Ireland. These rights are considered to be very important tools for environmental protection. At a time when people threaten their own existence by negative interference with the environment it is necessary that effective means for its protection exist. These means are access to environmental information and access to justice in environmental matters, which together can be called as environmental procedural rights. The first chapter of this thesis presents the sources of environmental procedural law and observes the development of law at international, European and national level. The most important international document in this field is the Aarhus Convention that was adopted in 1998 by most of the European countries and that provides for access to environmental information, access to justice and public participation in environmental decision-making. This convention has largely affected the law of the European Union, where several directives has been adopted, including most importantly the Directive 2003/4/EC on access to environmental information and the Directive 2003/35/EC on public participation in...
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Introduction to bio art
Šperanda, Nina ; SILVERIO, Robert (advisor) ; JANOŠČÍK, Václav (referee)
Bio art is a contemporary art practice that works with living organisms (except humans) or is created in cooperation with living organisms. It is relatively new art movement that is rooted deeply inside the history of evolution, domestication of animals and plants, technological discoveries, their development and science. Never before was art questioning its ethics and aesthetics in such profound, molecular and sub-molecular way.
In this work I will consider the historical influences and conditions that led to current developments in the field. I will also try to explain the problematics of defining what bio art is and try to clarify the associated terminology. Most relevant and ground breaking works in the field will be mentioned as some new recent developments.
Aesthetics of bio art is also an important subject often set aside by the ado around ethics of it. It is an extremely important subject to be considered when talking about bio art and I will try to sketch out a brief outline of it with references to philosophy and contemporary bio art theory.
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Interaction of apoprotein with heme side chains: A comparison of cytochromes P450 and NO-synthases
Fastová, Dagmar ; Hudeček, Jiří (advisor) ; Šulc, Miroslav (referee)
Cytochromes P450 and NO-synthases are two classes of heme-thiolate proteins, participating in catalysis of important biochemical reactions. In this work we analysed and compared both enzyme groups from the viewpoint of torsional angles of vinyl side chains of heme in positions 2 and 4. We used the Yasara programme to analyse the available crystalographic data. Whereas the torsional angle distributions for both vinyl groups in NO-synthases are pointing to a rather "conservative" conformations of both vinyls (between -30ř and 90ř for position 2, -150ř to 120ř for position 4, resp.) with little differences for individual forms of these enzymes, the mammalian cytochromes P450 display considerable differences between different families. In accordance with previous analyses is the distribution of torsional angles of 2-vinyls in most cases narrower (-120ř to 150ř) compared to 4-vinyls, which display more conformational flexibility (with torsional angles between -30ř and 180ř). In the second part of the Thesis we analysed the interaction of cytochrome P450 with its reaction partner, cytochrome P450 reductase. On the basis of crystalographic data we tried to prove, if the spatial positions of basic aminoacids, corresponding in the primary structure of various mammalian forms the those in the isoform 2B4...
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Dance education of preschool hyperactive children
Lacková, Ivana ; LÖSSL, Jiří (advisor) ; FRIČOVÁ, Marie (referee)
The topic of this diploma thesis is Dance education of preschool hyperactive children. Goal of research is to describe teaching of hyperactive children especially dance lessons.
There is described Attention Deficit Hyperactivity Disorder in the first part of thesis. There is written about special needs of children with ADHD focused on education.
Second part is focused on practical dance lesson of ADHD children. There is described example dance lesson based on theory and practical experience of experts.
The result of research is that supply of special lessons is very limited and it is big pity. In the diploma thesis was recognized that the education with special program should help to children with ADHD and it can be good supplement to standard medical therapy.
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The Risks of Premature Start of the Sexual Life of Women
SMUTNÁ, Kateřina
My thesis is called {\clqq}The Risks of Premature Start of the Sexual Life of Women``. This thesis has two parts, a practical one and theoretical one. The theoretical part describes the anatomy and physiology of woman´s body and its development. The start of the sexual life is an important part of women´s life and i tis influenced by many factors that can have both the positive and negative influence and these factors can be cultural, related to health or social. It is very important for young women considering starting their sexual life to have enough information about thein body and process going on in it and prevention of risks arising from unsuitable behaviour in adolescence. For a healthy sexual life it is important to be informed how to care for your body and health and in connection with it also for the reproductive health, a good sexual education is important too as well as biology at school connected with educational programmes for young people. I described the legislative conditions for starting the sexual live in the Czech Republic in theoretical part as well. I try find out in the practical part what risks are known to young women and what is their opinion as for their prevention. I chose the form of a qualitative research for my investigation and I thought of poll questions for this purpose and made a questionnaire and a set of questions out of it. There were two categories questioned, the first category were mothers whofilled in the questionnare, the second category were daughters who I had a standard conversation with. I tried to find out about the reproductive health and the sources of the information in this field the women use. I was trying to find out too whether women know the rsk factors influencing their sexual life. The end of my thesis is a list of enclosures.
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White crow dressed in black. Life's journey of František Jan Kroiher (1871-1948)
CUKR, Jiří
The present rigorous thesis is about the personality of František Jan Kroiher (1871-1948). He represented a very engaged person in a public area. His activity was very extensive and intervened in minor works in the country up to the contacts with the highest clerical and political circles. Individual chapters are devoted Kroiher´s childhood and study, career of the priest in the South Bohemia, his taking part in the reform efforts of the Czech clergy, work in a cooperative and economic sphere, a politic career in Agrarian Party and his extensive journalistic activities and sponsorship. At the end there is evaluation of Kroiher´s personality, followed by the list of sources and bibliography, index of names, a list of abbreviations and supplements. The rigorous thesis was based on prospecting quantity of the archival documents, period newspapers and literature. The present rigorous thesis is about the personality of František Jan Kroiher (1871-1948). He represented a very engaged person in a public area. His activity was very extensive and intervened in minor works in the country up to the contacts with the highest clerical and political circles. Individual chapters are devoted Kroiher´s childhood and study, career of the priest in the South Bohemia, his taking part in the reform efforts of the Czech clergy, work in a cooperative and economic sphere, a politic career in Agrarian Party and his extensive journalistic activities and sponsorship. At the end there is evaluation of Kroiher´s personality, followed by the list of sources and bibliography, index of names, a list of abbreviations and supplements. The rigorous thesis was based on prospecting quantity of the archival documents, period newspapers and literature.
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Etienne Decroux and his method Le Mime Corporel
Šimon, Lukáš ; VIZVÁRY, Radim (advisor) ; SOPROVÁ, Jana (referee)
This thesis discusses the life and work of Etienne Decroux, one of France's most important reformers of the theater and his method of Le Mime Corporel, which still forms the basis of modern pantomime. Decroux is known worldwide as an excellent mime, director, teacher, theorist and reformer of the theater. His contribution to the development of the European theater in the last century is very significant, especially for mime theatre. The work presents Decroux's personality and his contribution to the creation of the new method of mime theatre - Le Mime Corporel. Another task of this thesis is to explore the effects of different important personalities in the artistic development of Etienne Decroux, to describe the basic elements forming the method Le Mime Corporel and to compare it to other genres of movement theater.
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