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Measurement of thickness of skin and subcutaneous tissue in B-mode ultrasound image
Moldříková, Zuzana ; Štohanzlová, Petra (referee) ; Šrámek, Jaromír (advisor)
In the first part this bachelor thesis deals with theoretical analysis of terms, which are needed for understanding and algorithm proposition for skin thickness and subcutaneous tissue direct measurement. Second part of this thesis deals with implementation of algorithm. In six chapters there are described terms like skin and subcutaneous tissue, adiposity, Body Composition Analysis, ultrasound, B-mode and basics of image processing, which are going to be needed for processing of ultrasound image and algorithm creation. Chapter seven includes description of algorithm implementation and description of its structure. There is also analyzed clinical verification of functionality on phantom and some volunteers. The chapter is concluded with evaluation and discussion of gained results.
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Encapsulation of active substances into nanofibers and possibilities of their application
Procházková, Lucie ; Pernicová, Iva (referee) ; Skoumalová, Petra (advisor)
The master thesis was based on the optimization of the production of nanofiber covers and to gaine the product for subsequent functional use. The production of nanofiber covers was made by electrospinning and forcespinning from selected materials. Polyhydroxybutyrate, gelatin, chitosan and alginate were used as starting materials. After successful optimization, these materials were enriched with active ingredients ampicillin and ibuprofen for the functionalized use of covers for more effective wound healing. The theoretical part was focused on the issue of skin, healing processes, types of wounds and nanofibers, the characterization of selected starting materials for the formation of nanofibers was also mentioned. The practical part was based on the lengthy optimization of the preparation of fiber covers and later enriched with active ingredients. Furthermore, combined covers made of different materials with contents of both active ingredients were designed. This was followed by the characterization of all prepared covers from the point of view of stability in the short and long term. The gradual release of active ingredients was determined spectrophotometrically and by hifh performance liquid chromatography. It was also important to determine the antimicrobial activity of selected active substances. At the end of all testing, combined coatings containing both active ingredients were used for safety testing with human keratinocyte cells (HaCaT). Safety testing was based on determining the viability of human cells using the MTT test, to verify the LDH test. A scratch test was also performed, a wound healing test after the application of devised combined covers.
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Liveness Detection on Finger Using Various Light Sources
Vančo, Petr ; Kanich, Ondřej (referee) ; Drahanský, Martin (advisor)
The purpose of this work was to increase quality, security and reliability in biometric systems. This work presents electric and optical characteristics of a human finger. In the first part of this work you can find a theoretical basis about biometric systems, a skin and light. The next part is devoted to electric characteristics of a human finger. The third part of this work describes design, implementation and results of testing optical characteristics of a finger. In the end you can find information about some of the improvements for the future.
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Psychogalvanic reflex
Peterová, Soňa ; Harabiš, Vratislav (referee) ; Chmelař, Milan (advisor)
This thesis deals with the description, origin and possibilities of measurement psychogalvanic reflex. Also included will be the proces to measure the phenomen using either AC or DC. Psychogalvanic reflex is also associated with supraliminal stimulus. Activation or deactivation of sweat glands, controlled by free will of man, are responsive to this initiative. Attention is also given the characteristics of the electric current on human beings and the associated security scan on a living subject.
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Study of penetration of selected substances from elderberry extracts through biological membrane
Škubalová, Zuzana ; Němcová, Andrea (referee) ; Vespalcová, Milena (advisor)
This Diploma thesis focuses on the study of penetration of selected phenolic substances contained in extract from black elder (Sambucus nigra) via modelling systems represented by two types of synthetic membranes and a real system represented by a biological membrane. As a biological membrane, we have chosen skin from pig´s earlobe. Experiments of transdermal absorption and absorption through synthetic membranes were conducted through vertical (Franz´s) cells. Selected substances contained in the extract from black elder were rutin and chlorogenic acid. The main focus of this work was to determine how readily, or if at all, these two selected phenolic substances penetrate the skin and selected synthetic membranes. The percentage of absorbed amount of the substance was determined by HPLC analysis.
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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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Facial features recognition of unknown persons
Bartončík, Michal ; Babinec, Tomáš (referee) ; Horák, Karel (advisor)
This paper describes the various components and phases of the search and recognition of facial expressions of unknown persons. They are presented here as well as possible solutions and methods of addressing each phase of the project. My master’s thesis is designed to recognize facial expressions of unknown persons. For this thesis, I was lent industrial video camera, computer, and place in a laboratory. Furthermore, we introduce the color spaces and their use. From the lead representatives selects the most appropriate assistance for the use of Matlab and the proposed algorithm. After finding a suitable color space segments skin color in the image. The skin, however, surrounds the entire body and so need to be found, the separated parts of the image representing the color of skin, a face. Once you find a face is needed to find relevant points for the identification subsequent deformation to definition of facial expressions. We define here the actual muscle movements in different expressions.
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Liveness Detection of Human Hand from Multispectral Images
Kľučiar, Adam ; Drahanský, Martin (referee) ; Sakin, Martin (advisor)
This thesis is aimed at liveness detection from multispectral palm images. The theoretical part includes the importance of liveness detection in biometry systems and it discusses several methods of liveness detection, hardware and software-based. Subsequently, there is a summary of individual parts of the human skin and an explanation of the light spectrum, its division and the importance of individual parts for liveness detection and their effect on individual parts of the skin. The outcome of this thesis is the design and implementation of an algorithm capable of detecting anorganic hand imitations by their properties acquired from a set of images taken under the light of three different wavelengths.
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Contactless Device for Multispectral Hand Scanning
Hlavačka, Marek ; Kanich, Ondřej (referee) ; Sakin, Martin (advisor)
This bachelor thesis concerned to design and implementation of a contactless device for multispectral hand scanning. The first part was focused on the necessary knowledge of biometrics, properties of human skin and electromagnetic radiation. When designing the work, emphasis was placed on user comfort and quality of processing. A modifiable solution sensing both visible light and near infrared radiation has been developed. As part of the work, a database of hands of 21 different people was created with a size of 400 images.
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