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Analýza růstové schopnosti krůt ve vybraném podniku
JANDOVÁ, Lucie
The aim of the thesis was to evaluate the feeding parameters of hybrid B.U.T. 6 at selected farm. Six batches of tom turkeys were evaluated in this study. The growth intensity analysis was performed based on weekly weighing. The average live weight was 21.83 kg at the age of 21 weeks. The highest value was in the 3rd batch (22.18 kg) and the lowest value was in the 2nd and the 6th batch (21.62 kg). In comparison with the "Management guide", the differences in average weight from the 5th week of age to the end of fattening were evaluated as statistically significant. The average feed conversion ratio was registered 2.73 kg. The highest feed conversion was in the 1st batch (2.82 kg) and the lowest was in the 5th batch (2.62 kg). The average inflection point was reached in the 14th week (12.1 kg). First the inflection point was achieved in the 1st and 2nd batch (13.6 weeks; 11.7 kg and 13.7 weeks; 11.4 kg) and at the latest in the 4th batch (15.5 weeks; 13.6 kg). The average maximum daily gain was reached 203.1 g (13.6 weeks). The highest was in the 3rd batch (204.9 g; 13.6 weeks) and the lowest was in the 4th batch (195.3 g; 14.3 weeks). The average mortality rate was found 6.39%. The highest mortality rate was in the 6th batch (8.44%; 858 pcs) and the lowest was in the 4th batch (4.50%; 457 pcs).
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Mathematic modeling of microorganisms growth
Kecskésová, Viktória ; Obruča, Stanislav (referee) ; Babák, Libor (advisor)
The aim of Bachelor thesis was mathematical modeling of microbial growth, namely Lactobacillus casei CCM 4798. Microorganism was cultivated in Erlenmeyer flasks by technique batch cultivation. Used media were composed with recommended chemicals for the growth of lactobacilli and various concentrations of substrate. Subsequently, the growth curves were determined in two ways, as the dependence of optical density to time and the concentration of dry biomass per time. It were selected three suitable models, model of exponential growth, Monod model and Tessier model. Further, using MATLAB, application Curve Fitting Toolbox and mathematical models was modeled exponential phase of growth curves. According to statistics in MATLAB it was founded that the best fit was enabled of model of exponential growth.
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Preparation of selected microbial metabolites from waste materials
Jechová, Iva ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This diploma thesis deals with the biodegradation of whey on selected microbial products (carbohydrates, lactic acid, acetic acid and ethanol) thermophilic bacteria of genus Thermus aquaticus and mesophilic bacteria of genus Lactobacillus casei and Bacillus coagulans. For cultivation was used as medium whey, from which the proteins were removed and which was enriched with nutrients. On the basis of culture in the fermentor were determined growth curve and the HPLC method were determined individual bioremediation products.
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Growth characteristics of thermophilic microorganisms
Kuchtová, Ludmila ; Vítová, Eva (referee) ; Babák, Libor (advisor)
The aim of this work was focused on study of influence of cultivation conditions on growth of thermophilic bacteria Thermus aquaticus, Thermus scotoductus, Geobacillus thermodenitrificans and Geobacillus thermocatenulatus in cultivation medium recommended by Czech Collection of Microorganisms (CCM). The change of concentration of biomass during cultivation with various pH of media, cultivation temperature, agitation rate and with addition of glucose to medium during cultivation in Erlenmayer flasks was observed. Results served for determination of optimal growth conditions for each microorganism. After optimal growth conditions were obtained, cultivations in fermenter were performed. Growth curves were made from values of absorbance and dry weight of samples.
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An antimicrobial activity of Stevia rebaudiana extracts
Mlatečková, Tereza ; Zemanová, Jana (referee) ; Vespalcová, Milena (advisor)
This master thesis is oriented on study antimicrobial effects extracts and macerates from cure Stevia rebaudiana Bertoni. Teoretical part describes basic information about plant Stevia, summary of health significant matters contained in Stevia and posobilities preparing extracts from Stevia. Antimicrobial effects extracts and macerates from cure Stevia were testing on food-borne bacteria (Bacillus cereus and Micrococcus luteus) and yeasts (Geotrichum candidum and Hansenula anomala). Microorganism, extracts and macerates were chosen on basis previous study (Study of antimicrobial effects Stevia Rebaudiana extracts, Eva Rakovská). For screening antimicrobial activity were determined the growth curves by using turbidimetrie for bacteria and direct treetment metod of cells number for yeasts. Antimicrobial effects were confirmed aplication with diffusion pit method on the agar ranges. From the results flow the testing extracts and macerates from stevia analysed antimicrobial effects. The best effect was demostrated on macerates and the most sensitive was bacteria Micrococcus luteus with the best inhibitoring effects.
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Study of growth and metabolic properties of selected microorganisms
Hudečková, Helena ; Šupinová, Petra (referee) ; Babák, Libor (advisor)
The aim of this Bachelor thesis was study of the growth and production of selected metabolites of microorganisms, namely Lactobacillus casei and Saccharomyces cerevisiae. Microorganisms were cultivated in Erlenmeyer flasks by using the recommended media for their growth. The growth curves were determined from the values of optical density and concentration of biomass. Samples were then analyzed by HPLC for determination of the content of glucose and ethanol in S. cerevisiae and the content of glucose and lactic acid by L. casei.
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Metabolite production by some strains of industrial yeasts in various phases of cell growth
Jankeje, Kristína ; Kubešová,, Jitka (referee) ; Kočí, Radka (advisor)
Presented bachelor thesis is focused on industrial application of chosen yeast strains. Principal interest of work is to study production of primary and secondary metabolites during individual growth phases. Optimal growth conditions as well as influence of exogenous stress factors (mainly oxidative and/or salt stress) on cell growth and yeast metabolism are discussed. In experimental part growth curve of industrial strain Phaffia rhodozyma was determined. Biomass increase (maximum in 90th hour 5,441 g/l), astaxanthin production (secondary metabolite) and/or ergosterol biosynthesis (primary metabolite) were observed. The best ration of astaxanthin to total carotenoids was 50 %. Next studied metabolite was ergosterol, its total amount in dry biomass was 0.11 %. In conclusion astaxanthin amounts produced in optimal growth conditions were compared with yields obtained under stress cultivations. Results of stress experiments illustrate positive influence of stress factors on cell growth as well as on astaxanthin biosynthesis. Low concentration of salt (2% NaCl) added in inoculum with 5 mM hydrogen peroxide in production medium would be the best combination in industrial applications.
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