|
| |
|
|
Krystalizačně-strukturní studie bakteriofágového lysinu ECD7
REINDLOVÁ, Žaneta
This bachelor thesis is focused on the crystallization of newly acquired bacteriophage lysine ECD7. Bacteriophage lysine ECD7 could be one of a new alternative class of antibacterial agents possibly used against infectious diseases. The main goal of this work was to manage the basic crystallization methods, then apply them for crystallization of ECD7 lysine and produce a suitable crystals for X-ray diffraction analysis. Data from X-ray diffraction analysis will help clarify the structure and function of ECD7 lysine.
|
|
|
Study of the influence of nanoparticles on water properties
Šram, Matěj ; Komendová, Renata (referee) ; Řezáčová, Veronika (advisor)
This work deals with the study of the influence of platinum nanoparticles with a size of 3 nm on the properties of water in a porous system. SiO2 with a defined pore size was used as a porous system, which can simulate the mineral component of the soil. The theoretical part deals with the properties of free and porous water and the impact of nanoparticles on the environment. In the experimental part, the properties of free water, water in the porous system and the influence of the presence of platinum nanoparticles on these systems are investigated using differential scanning calorimetry (DSC). The observed processes were melting, solidification and evaporation, and derived quantities such as crystallization and melting point.
|
|
| |
|
|
Salts of 2-aminoethanol and ferrocene-based acids
Zábranský, Martin ; Štěpnička, Petr (advisor) ; Němec, Ivan (referee)
Combination of the compact but sterically flexible ferrocene scaffold with intermolecular binding potential and conformational variability of (2-hydroxyethyl)ammonium structural motif was utilised in the construction of solid crystalline materials. Crystallisation in systems containing ferrocenecarboxylic, 2-ferrocenylacetic, 3-ferrocenylpropionic, 3-ferrocenyl- acrylic, 3-ferrocenylacrylic, 3-ferrocenylpropiolic, ferrocene-1,1'-dicarboxylic or ferrocene- sulfonic acid with 2-aminoethanol afforded crystals of the corresponding salts. The resulting crystalline products were characterised with the usual methods (proton nuclear magnetic resonance, infrared spectroscopy, elemental analysis) and their crystal structures were determined by means of single-crystal X-ray diffraction analysis. The crystal structures of the salts mentioned above usually contain rather complicated two dimensional networks of charge-assisted hydrogen bonds. With the aim of studying potentially more simple hydrogen-bonded structures, additional crystallisation experiments were conducted in systems of ferrocenecarboxylic acid and 2-(methylamino)ethanol or 2-(dimethylamino)ethanol. These experiments yielded simple salt of the former amine and adducts of salts of both bases with ferrocenecarboxylic acid in the ratio of 1:1. The...
|
|
|
Quantitative description of textures in volcanic rocks: Case study of trachybasalt from Dubičná
Ditterová, Hana ; Dolejš, David (advisor) ; Rapprich, Vladislav (referee)
- iii - ENGLISH ABSTRACT Quantitative description of textures, in particular, the distribution of grain sizes, is becoming a powerful tool for interpreting kinetic processes that govern crystallization of igneous rocks. Variation in grain size are frequently represented by logarithm of population density vs. linear crystal size, where the majority of igneous rocks forms a linear trend. Its slope provides an estimate of characteristic grain size, or growth rate and crystallization time, whereas the intercept provides the nucleation density. The log-linear trend is predicted to result from kinetic nucleation and growth processes only, whereas its modifications such as kinks or curvature are due to mechanical separation or accumulation of crystals, magma mixing or Ostwald ripening (coarsening) in the presence of melt. In order to investigate the true, three-dimensional crystal size distribution, the raw two-dimensional data acquired from thin sections or rock slabs must be processed by various stereological methods. We illustrate application of quantitative textural measurements to distribution and origin of clinopyroxene crystals in a trachybasaltic lava flow from Dubičná near Úštěk. This effusion belongs to Cenozoic volcanic products in the Bohemian Massif, specifically to its main Eocene-Miocene stage in...
|
|
|
Characterization of recombinant fragment of an antibody against CD3 marker.
Písačková, Jana ; Maloy Řezáčová, Pavlína (advisor) ; Obšil, Tomáš (referee)
Monoclonal antibody MEM-57 recognizes CD3 antigen expressed on peripheral blood T-lymphocytes. CD3 surface glycoprotein complex associates with T-cell receptor and is responsible for the transduction of activation signal. Antibody MEM-57 has, therefore, a large diagnostic and therapeutic potential. It could be used in autoimmune diseases diagnostics, for classification of T-cell leukemias and, as an immunosuppressant, in transplantation. The most promising therapeutic use of MEM-57 antibody would be the construction of a "Bispecific T-cell Engager" (BiTE) antibody format with potential application in cancer therapy. In this format, single-chain variable fragment (scFv) of MEM-57 would be fused with an anti-tumor antigen scFv. The thesis is focused on biochemical and biophysical characterization of MEM-57 antibody scFv fragment. Recombinant antibody fragment scFv MEM-57, equipped with the pelB leader sequence, c-myc tag and His5 tag, was produced from a pET22b(+) vector into the periplasmic space of E. coli BL21 (DE3). Two-step purification protocol, employing nickel chelation affinity chromatography and ion-exchange chromatography, was developed to obtain high yield of pure protein. The antigen binding activity of scFv MEM-57 was confirmed by flow cytometry. Structural information on scFv MEM-57...
|
|
|
Preparation and crystallization of a metabolic repressor LutR from Bacillus subtilis
Soldánová, Anna ; Maloy Řezáčová, Pavlína (advisor) ; Kutá-Smatanová, Ivana (referee)
Metabolic transcriptional repressors are proteins controlling transcription of specific genes involved in bacterial metabolism. These proteins typically consist of two domains: N-terminal DNA-binding domain (DBD) and C-terminal effector-binding domain. When an effector (usually a metabolite molecule) binds to the protein, the conformation of the protein is changed. This causes a change in affinity to its DNA operator and that subsequently modulates the transcription of genes of the specific metabolic pathway. LutR belongs to the GntR family of bacterial transcriptional regulators. In undomesticated strain RO-NN-1 of Bacillus subtilis, LutR regulates transcription of genes required for L-lactate utilization. Interestingly, LutR from laboratory strains PY79 and 168 has a different function. Due to a mutation, it lacks the first 21 amino acids and this alters its DNA recognition specificity. This LutR variant acts as a global regulator and regulates many genes associated with transition from exponential growth to stationary phase of bacterial population. Knowledge of 3D structure of LutR with DNA could elucidate the impact of this short deletion in LutR DBD on the mechanism of DNA recognition. In this work, the DBD of LutR from undomesticated strains of B. subtilis was prepared by heterologous...
|
|
|
Zero liquid discharge systems
Štylárková, Petra ; Máša, Vítězslav (referee) ; Touš, Michal (advisor)
The bachelor thesis contains a description of zero liquid discharge systems. It describes the process, which has three main parts, it is pretreatment, preconcentration and evaporation. In each parts there is a basic description of the principle of the metod, their use etc.
|
|
|
Crystallization of coating systems based on epoxy resins.
Seidlová, Michaela ; Dohnálek,, Pavel (referee) ; Petránek, Vít (referee) ; Drochytka, Rostislav (referee) ; Bydžovský, Jiří (advisor)
Crystallisation of epoxy resins based on bisphenol A and bisphenol F is the natural property of these oligomers. However, manufacturers of paints and other systems based on these epoxy resins, strive to slow down the crystallisation process as much as possible, thereby prolonging the shelf life and improving the competitiveness of their products. The main aim of the thesis is generalisation of the factors influencing the crystallisation process of epoxy resins based on bisphenol A and bisphenol F, verifying new approaches to the possibility of influencing the crystallisation process and determining the possibility of using a certain degree of epoxy resins crystallinity based on bisphenol A and bisphenol F in the preparation and production of epoxy resinbased coating systems.
|
guest ::
