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Characterization of fungal community in spruce (Picea abies) litter using cultivation and T-RFLP
Kolářová, Zuzana ; Koukol, Ondřej (advisor) ; Brabcová, Vendula (referee)
Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....
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Long-term disturbances in temperate mountain forests and their representation in various proxy data
Pavlišová, Adéla ; Kuneš, Petr (advisor) ; Moravcová, Alice (referee)
In this work, I present the main disturbance agents affecting temperate mountain forests in Central Europe - wind, bark beetles and fires, and I draw attention to their interdependence. I primarily deal with the description of disturbances, their consequences, ecological contribution, and climate influence on their action. With climatic warming, the extent, intensity, and frequency of disturbances increase. Hence, it is important to understand their role in the past to prepare for the future development of our forests. Proxy data (indirect data) provide past evidence for disturbances, but their interpretation value differs. They inform us about a different time and spatial scale with varying degrees of accuracy. All the methods that I mention in my work provide us, to some extent, with climate proxy data that is closely related to the disturbance regime. Only some directly prove the disturbance, for example, through sedimentary charcoals findings. My work aimed at indicating possible complications but also the advantages that arise when combining individual methods.
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Legal regulation of forest protection in the context of bark beetle outbreak and climate change
Matoušek, Oliver ; Pokorný, Jiří (advisor) ; Sobotka, Michal (referee)
Legal regulation of forest protection in the context of bark beetle outbreak and climate change The aim of this thesis is to analyse current legal regulation of forest protection in Czech law, primarily areas related to ongoing bark beetle outbreak with the focus on forest management. In the interest of presenting the topic in its broader context, elementary aspects of forestry and other nature sciences are briefly discussed. Author also evaluates and gives his opinions on the current legal regulation and its imperfections throughout the thesis. The thesis is divided into four main parts. The first part serves as a brief introduction into the subject matter of the thesis i.e. legal regulation regarding forest protection. The first chapter of this part discusses the term "forest" from the point of view of natural sciences and details the importance of forest and different forest functions. Second chapter of the first part discusses factors harmful to forests, mainly bark beetles, droughts and climate change and the concurrent effect of those factors. Second part of the thesis maps the evolution of Czech legal regulation and tries to find parallels between previous and today's legal regulation. Oldest discussed legal document is the Maiestas Carolina of the 14th century. Thesis then moves on to the...
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Neglected aspects of bark beetle (Coleoptera: Scolytinae) ecophysiology
DAVÍDKOVÁ, Markéta
The thesis describes several unknown aspects of the spruce bark beetle, Ips typographus (L.), and the double-spined bark beetle, Ips duplicatus (Sahlberg.), life-cycles and ecophysiology. The first study focuses on I. typographus and its dispersal under epidemic conditions in the National park Šumava and introduces a novel method of fluorescent marking and detection of captured specimens. The second study is focused on ability of I. typographus to establish so-called sister-broods, i.e. re-emergence of females that are capable to continue egg laying without a need to mate again. The importance of sister-broods becomes apparent mainly in recent hot and dry vegetation seasons, which is demonstrated by a comparison of recent and historical data. The third study focuses on temperature-dependent development of I. duplicatus under laboratory conditions by the means of sandwich method. Altogether, the studies underline practical importance of ecophysiological studies on bark beetles as one of the tools for their effective management.
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The impact of a bark beetle gradation on the occurrence and feeding ecology of a three-toed woodpecker (Picoides tridactylus) in the Šumava National Park
Navrátil, Martin ; Fuchs, Roman (advisor) ; Reif, Jiří (referee)
The main objective of this study was to find factors that influence the occurrence of three-toed woodpecker (Picoides tridactylus) in Šumava at a time when the bark beetle gradation caused by Kyrill subside. The research was conducted on five transects. To prove the presence of three-toed woodpecker was opted the method of an acoustic provocation, which reliability was well proven. The hypothesis was that the three-toed woodpecker prefers the trees infested by bark beetle instead of healthy trees. Surprisingly, however, he also prefered the stands where the main attack took place several years ago. Regardless of their health status the tree- toed woodpecker preferred non-intervention stands instead of the on- going management forest. Among other factors, the exposure was applied (preferred south and west) and elevation (median, minimum altitude of about 1100 m.). Another important result is that Picoides tridactylus prefers for its food stands still alive or just dead spruce trees infested by the bark beetle. Overall, it appears that the three-toed species has narrowly defined biotope demands, which are linked to the natural spruce forest unaffected by people. The results of this study could be used to better understanding of the ecology three-toed woodpecker, eventually can help to create a...
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Historical occurrence of bark beetle (Coleoptera: Scolytinae) in the Bohemian Forest, Czech Republic, and possibility of its study in lake sediments
Vytisková, Markéta ; Hořická, Zuzana (advisor) ; Hruška, Jakub (referee)
The bachelor's project is closely related to the problem of bark beetle calamities in the Bohemian Forest National Park in the Czech Republic, and to a possible use of paleolimnological methods for their historical reconstruction. The importance of subfossil beetles as a proxy in assessing past changes in the nature is growing, and the occurrence of remains of Scolytinae in lake sediments has been confirmed - also in the Bohemian Forest paleolake Stará jímka. The thesis is a literature review of (1) the relationship between spruce forests and bark beetle gradations in the area, and (2) chitinous remains of bark beetle and other Scolytinae preserved in lake sediments, and their paleoecological implications. Key words Bohemian Forest (Czech Republic), spruce forests, bark beetle, paleolimnology, subfossil Coleoptera, paleolake Stará jímka
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