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Importance of biotic and abiotic components in feedback between plants and soil
Hanzelková, Věra ; Pánková, Hana (advisor) ; Knappová, Jana (referee)
The plant-soil feedback affects the forming of a plant community. Plants affect their own species as well as other species. The plant-soil feedback can be both positive and negative. Plants affect soil, change its properties, and the soil affects the plants reciprocally. Soil components can be divided into biotic and abiotic ones. The abiotic component is represented by physical and chemical properties of the soil. The main properties are the soil structure, the soil moisture, the soil temperature, the soil pH and the amount and availability of nutrients. The biotic component is composed of soil biota. The individual organisms can function as decomposers, symbionts and pathogens. The majority of soil organisms is composed of microorganisms, the most important of which are fungi and bacteria. The effect of the abiotic component in plant-soil feedback is mostly nonspecific, while the effect of the biotic component is more specific. These components interact with each other and determine the resulting effect on the plants and the type and intensity of interaction between various plant species. The aim of this thesis is to show the importance of these components in the plant-soil feedback. Key words: plant-soil feedback, abiotic factors, biotic factors, soil structure, soil water, soil temperature, pH,...
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European black alder (Alnus glutinosa L. Gaertn.) in symbiosis with Frankia and their growth on post-mining heap soils
Buchbauerová, Lucie ; Frouz, Jan (advisor) ; Roubíčková, Alena (referee)
Alder (Alnus glutinosa L. Gaertn.) is a species of a pioneer plant usually colonizing sites in the early stage of ecological succession, such as spoil heaps after open-cast brown coal mining in the Sokolov mining district in north western Bohemia, Czech Republic. These spoil heaps are very poor in nutrients available for plants, yet alders grow in a mutualistic relationship with actinomycetes Frankia, which live in root nodules of the alder plants. Frankia are able of fixing atmospheric nitrogen (N2) to ammonia (NH3) molecules, which can be then assimilate by alders, via enzyme nitrogenase. Thus, in the early stages of succession, alders have a competitive advantage to other non- fixing plant species living only on nitrates (NO3 - ) and ammonia ions (NH4 + ) present in soils. The aim of this study was to conduct and assess two greenhouse experiments. The first experiment studied the response of alder growth to presence of Frankia and arbuscular mycorrhizal fungi of order Glomerales. The performance of alder growth was significantly higher when alders were inoculated with both Frankia and mycorrhizal fungi in comparison to when alders have grown on their own or only with a mycorrhizal symbiont - both on 14 and 60 years old soils from Sokolov mines. In the second experiment, soil pH and iron (Fe) and...
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Využití mykorhizních hub ve školkařské produkci okrasných dřevin
Linhartová, Anna
This thesis discusses the use of mycorrhizal fungi in nursery production. In the literature review there is summarized current knowledge regarding mycorrhiza and nursery production of ornamental plants. As a model plant for the experiment was used Spiraea bumalda "Anthony Waterer" and the experiment was conducted in the grounds of The Department of Breeding and Propagation in Lednice. There was applied mycorrhizal product Symbivit when planting in batch 6 g, 3 g, 1,5 g and 0 g. The plants were evaluated by parameters as plant height, diameter of root collar, the number of shoots. Then there were evaluated mycorrhizal colonization and volumes of roots balls in the laboratory. The experiment was described and evaluated statistically. The experiment resulted in the fact, that the more mycorrhizal product Symbivit is applied, the greater was the colonization. The mycorrhizal product had a positive effect on plant growth. The plants treated with product Symbivit in batch greater than 3 g proved a statistically significant difference in the monitored parameters compared to the control. There is also a economic viewpoint, the price of plants increases after the application of mycorrhizal product Simbivit.
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Plant sugar metabolism and arbuscular mycorrhizal symbiosis
Konečný, Jan ; Jansa, Jan (advisor) ; Gryndler, Milan (referee)
The study of arbuscular mycorrhizal symbiosis (AMS) - the mutualist relationship between the most of land plants and evolutionary old fungal group Glomeromycota - is becoming a prestigious topic. The prevalence of and extent of physiological action of AMS on plants is very interesting for the plant biology itself, but its importance grows, notably in time of global climate change, frequent soil degradation and ascending exhaustion of mineral fertilizer reserves. Although the flows in AMS of some minerals, like of phosphorus was enlightened, carbon exchange between the symbionts is still poorly understood. In this experimental work, I utilized the boom of molecular and bioinformatic methods in the quest for completely unexplained carbon flows. The organisms used include barrel medic (Medicago truncatula), the model legume for symbiotic relationships, biotic, and abiotic stresses; Rhizophagus irregularis, the widely used fungus for such experimental studies of AMS; and Sinorhizobium meliloti, the nodulating nitrogen-fixing bacterium compatible with the barrel medic. Two variants - mycorrhizal (M+) and non-mycorrhizal (NM) plants were subjected to several levels of analysis. I have checked the variants, did the measurements of phosphorus and nitrogen contents, as well as I probed the plants with...
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How works mycorrhizal pathway in phosphorus acquisition?
Konečný, Jan ; Jansa, Jan (advisor) ; Janoušková, Martina (referee)
The plant takes up mineral nutrients by its roots from the soil. Minerals are available in the soil solution in the form of ions and can be translocated across the cytoplasmatic membrane into the cell of the plant. If the plant is alone, we talk about direct pathway. In case of some minerals, which are not available so easily, the plant finds solution in mutualistic relationship - the mycorrhizal symbiosis. By mycorrhizal pathway the nutrients come from much larger volume of soil, than the plant could reach, and intake of low-mobile elements, like phosphorus, becomes facilitated. How is the phosphorus taken up through mycorrhizal pathway, how is it regulated and accessory knowledge are the topic of this bachelors thesis.
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Importance of arbuscular mycorrhizal fungal communities for the growth of selected plant species on an abandonned field
Voříšková, Alena ; Janoušková, Martina (advisor) ; Gryndler, Milan (referee)
The thesis deals with the effect of arbuscular mycorrhiza (AM) on the growth of selected plant species at a locality in České středohoří. This locality is characterized by close neighborhood of a semi-natural dry grassland and a former field abandonned in the 1990s, typical for the mosaic of biotopes in the region. The study is based on previous findings that some plant species, which are common at the semi-natural dry grasslands, do not colonize the abandoned fields. As AM is an important factor affecting diversity and productivity of plant communities we hypothesized that this phenomenon could be related to changes in AM fungal communities at the abandoned field. The hyphothesis was tested in a greenhouse pot experiment with three taxonomically related pairs of plant species, always one species growing at the abandoned field and the second one not. Growth and phosphorus uptake of the plants was followed in soils of both biotopes after factorial inoculation with AM fungal communities from both biotopes. The experiment was complemented by description of AM fungal communities in the roots of six plant species pairs from the locality using terminal restriction fragment length polymorphism (T-RFLP). The greenhouse experiment revealed positive mycorrhizal response in all plant species, but the origin...
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Vliv duální mykorhizy na příjem těžkých kovů vybranými dřevinami čeledi Salicaceae
Kuchár, Michal ; Mrnka, Libor (advisor) ; Kolaříková, Zuzana (referee)
3.2. Abstract Soil contamination by heavy metals represents rather serious environmental problem for both human health and an environment itself. One of the perspective technologies dealing with this threat that only recently has been intensely developed is phytoremediation by means of short rotation coppice plantations. As plants used in this technology (mostly poplars and willows) host two major groups of mycorrhizal fungi substantially influencing plant physiology it is important to study plant-mycobiontheavy metals interactions rather than just plant-heavy metals interactions. The present thesis aimed to contribute to the growing knowledge of the field by search for suitable mycobionts of poplar or willow tolerant to heavy metals, by evaluating an activity of the key antioxidative enzyme in selected mycobionts and by looking at physiological responses of plant hosts to their mycobionts in a soil polluted by heavy metals. The first experiment in vitro focused on screening of morphometric criteria of fungi growing on solid growth media amended with mixture of heavy metals. Based on the results, several tolerant ectomycorrhizal strains were chosen for the next inoculation of fast growing trees serving phytoextraction and phytostabilisation strategies. The second, re-synthetic experiment was conducted in...
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Specificity between partners in arbuscular mycorrhizal symbiosis
Voříšková, Alena ; Janoušková, Martina (advisor) ; Püschel, David (referee)
Arbuscular mycorrhiza (AM) is a symbiotic interaction between vascular plants and arbuscular mycorrhizal fungi from phylum Glomeromycota. AM symbiosis is considered as a nonspecific interaction, because AM fungi are able to colonize roots of all plant species which are hosts for this type of symbiosis, nevertheless certain specific interactions exist between the partners. The specificity can be evident on the level of the formation of mycorrhiza, which means that different species of plants can vary in the composition of fungal community in their roots or AM fungi can differ in an ability of colonization and sporulation depending on host plant species. The specificity can also manifest as different functionality of the symbiosis in dependence on the particular partners affecting the mycorrhizal response of the host plants. All of these specific interactions can have an impact on the coexistence and diversity of plants in communities.
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