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Uses of active compounds from honey in cosmetic products
Balášová, Patricie ; Langová, Denisa (referee) ; Skoumalová, Petra (advisor)
The bachelor thesis was focused on the preparation and characterization of extracts and liposomes with active substances from chosen types of honey with the aim to apply these extracts to the cosmetic products. The extraction of active substances from chosen material was optimized during the work. As the most suitable organic solvent for extraction was chosen diethyl ether. All the extracts were tested on the content of total polyphenols, sugars and antioxidation activity. The final samples were also tested on the content of flavonoids. Extracts were encapsulated to the liposomes, which were tested especially for their antioxidation activity, size and stability. Then, extracts and liposome particles were applied to the cosmetic products. Cosmetic creams and gels were prepared and tested for antioxidation activity and antimicrobial activity against the strains Serattia marcescens and Micrococcus luteus. Antimicrobial activity of gels was proved especially against the strain of grampositive bacterias – Micrococcus luteus. From this point of view another one testing strain was chosen – Propionibacterium acnes. Finally, the chosen liposomes were tested for their contact with human cells, immortalized keratinocytes HaCat. No cytotoxic effect was observed and tested materials can be applied to cosmetics against acne.
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Synthesis and antiinfective evaluation of substituted N-(pyrazin-2-yl)benzenesulfonamides
Paredes De La Red, Cristina ; Doležal, Martin (advisor) ; Kučerová, Marta (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Author: Cristina Paredes de la Red Supervisor: prof. PharmDr. Martin Doležal, Ph.D. Title of diploma thesis: Synthesis and antiinfective evaluation of substituted N-(pyrazine-2- yl)benzenesulfonamide Tuberculosis (TB) is among the ten leading causes of death, especially in developing countries. Even though it is an old disease with established treatment regimen, there has been an increased resistance to anti-TB drugs 1 . The anti-tubercular pyrazinamide has caught the attention of researchers as the different theories for its mechanism of action have made it an interesting entity for further investigation. Here we will discuss N-(pyrazine-2-yl)benzenesulfonamides (General structure is presented in the Figure below) as a new derivatization approach based on synergism methodology between pyrazinamide and sulfonamides. Sulfonamides exert their antimicrobial effect by competitive inhibition of folic acid synthesis and subsequent inhibition of bacterial growth and reproduction 18 . I have contributed to the synthesis and purification of 8 compounds in a series of total 22 N- pyrazinylsulfonamides. Two of the prepared compounds showed activity against Mycobacterium kansasii [2a (MIC...
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Fabrication and characterisation of silver decorated TiO2 nanotubes for biomedical applications
Bílek, Ondřej ; Horynová, Miroslava (referee) ; Fohlerová, Zdenka (advisor)
This diploma thesis summarizes knowledge from the fields of synthesis, characterization and application of titanium dioxide nanotubes and its combination with silver nanoparticles for biomedical purposes. Basic protocols of working with cell cultures and bacteria are also included. Experimental part of this diploma thesis focuses mainly on the synthesis of tubular structures made of titanium dioxide via anodic oxidation of 500nm titanium layer and their subsequent decoration with silver by electrodeposition. Last section of the experimental part is devoted to testing of antibacterial properties of the new material and examining the effect of different silver concentrations on the adhesion of MG-63 cells. All results are compared to reference samples consisting of titanium dioxide nanotubes without silver.
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Characterization and application of some plant antimicrobial substances
Bendová, Agáta ; Bokrová, Jitka (referee) ; Skoumalová, Petra (advisor)
The main goal of this bachelor thesis was the study of antioxidant and antibacterial effects of natural plant extracts and liposomes. The theoretical part was focused on description of natural antimicrobial and antioxidant substances from selected herbs and spices. Also bacterial resistance and methods used in work were described. In the experimental part basic characteristics of extracts from 12 kinds of herbs and spices (oil, alcohol and aqueous) were analyzed. Mainly content of polyphenols, flavonoids and the total antioxidant activity were determined. All extracts were also encapsulated into liposomes and the encapsulation effectivity, long-term stability and size and stability of liposomes were determined. The size of prepared liposomes was in range of 100 nm to 200 nm. Stability of the liposome particles was measured using a zeta potential and all prepared particles exhibited very good stability, but in term of long term stability were liposomes relatively unstable. Antibacterial activity was tested against Gram-positive Micrococcus luteus and Gram-negative Serratia marcescens. Encapsulation of extracts into liposome particles has greatly increased their antimicrobial effect and liposomes containing oil extracts from the goat, nutmeg and pepper and liposomes containing alcoholic extracts from star anise and cloves were the most effective against both strains. The prepared liposomes could be used as alternative to antibiotics and could be found application in the form of various antimicrobial gels for the cosmetic or pharmaceutical industry.
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