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Geometry of protein moleculs
Novotný, Jan ; Maděránková, Denisa (referee) ; Škutková, Helena (advisor)
Objective of this work was to study the spatial arrangement of protein molecules, with their extraction, notations and variables used in the course descriptions. Prediction of protein structures is very important for determining the future function of proteins. Another objective was to create a function in MATLAB that will graphically represent the spatial arrangement of the backbone structure of the protein by form Ramachandran plot. Along with this function create another function to render the side-chains of amino acids and function to calculate the stereochemical quality. Finally, create a program for a complete assessment of the spatial arrangement of the protein molecules and calculating the stereochemical quality of and test program for protein structures from public databases RSCB PDB.
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Effect of magnetic field on laser-induced plasma characteristics
Vrábel, Jakub ; Pořízka, Pavel (referee) ; Prochazka, David (advisor)
This work is based on topic of laser induced breakdown spectroscopy „LIBS“ and its application to chemical analysis of materials. Paper presents a brief theoretical basis of LIBS with focus on effect of magnetic field to plasmas and modeling spectras for calib-ration-free LIBS analysis. Considerable part of work is dedicated to practical demon-stration of modeling spectras, using Matlab and interpretation of experimental results. The final output is automatized method for evaluation of spectras, which could leads to calibration-free LIBS analysis. Also there is an effort to increase sensitivity of method.
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Fingerprint Liveness Recognition
Lodrová, Dana ; Orság, Filip (referee) ; Drahanský, Martin (advisor)
This document deals with presentation of nowadays software and hardware methods used for fingerprint recognition with focus on liveness testing and thereafter it deals with description of my solution. In order to describe results obtained from study of technical literature, we discuss important terminology of biometric systems at first and further main principles of fingerprint sensors used in practice are shown. From overviewed methods of liveness detection we underline one method based on perspiration (researched by BioSAL laboratory) and one spectroscopic method researched by Lumidigm Corporation. The study of liveness testing methods inspired me to creation of new type fingerprint sensor which has built-in livennes testing method based on two characteristic properties of living human tisue. In order to test this sensor, we discuss nowadays sensor deception method. It follows from their analysis, that newly designed sensor should be theoretically resistant to each of them.
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Design of compact spectrograph with shiftable spectral range based on transmission gratings
Malina, Jan ; Pořízka, Pavel (referee) ; Novotný, Jan (advisor)
The aim of this diploma thesis is to design a compact spectrometer with a transmission grating and a shiftable spectral range for the method of laser-induced breakdown spectroscopy (LIBS). The work in the theoretical part contains a basic description of spectroscopy and spectroscopic methods of LIBS, spectrometer and its individual parts. The practical part describes the spectrometer design procedure, introduces selected components and comments on the reason for their selection. Selected components are assembled into the final form of a spectrometer, which is further tested with the help of a simulation program to confirm its functionality. The result of the work is a comprehensive overview of the individual components of the spectrometer, aspects of its construction together with instructions and advice on its assembly, along with an example in the form of a designed set of commonly available catalog components.
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Properties of graphenoid layers
Mach, Radoslav ; Čech, Ondřej (referee) ; Vaněk, Jiří (advisor)
Master thesis “Properties of graphenoid layers” deals with materials of graphenoid nature such as graphene, graphene oxide and its reduced state. The paper effectively summarize basic theoretical knowledge in the first half of its range. In the second half the project deals with practical part consisted of experiments with application of graphene oxide solvents, its analysis and especially comparing properties of non-reduced graphene oxide with its chemically reduced form. Material is examined in a form of applied thin layers on different substrates.
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Analysis of composite system with different filler ratio
Mydlář, Marek ; Frk, Martin (referee) ; Rozsívalová, Zdenka (advisor)
This master’s thesis applies dielectric relaxation spectroscopy to analyse impact of climatic changes on dielectric properties of composite material samples (TSA 220S varnish combined with 60.030 mica). Experiments aim to analyse effect of mica composite mass ratio (0, 4, 8 and 16 % of mica), relative humidity (0, 33, 55, 65, 75 and 95 %) and temperature (23, 30, 40 and 50 C) on complex permitivity as a function of frequency.
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Nanoparticles preparation by green synthesis
Kortusová, Dominika ; Valušová,, Eva (referee) ; Šimšíková, Michaela (advisor)
This thesis deals with green synthesis of noble metals nanoparticles. The first part contains secondary literature research which maps both the traditional and newly developed techniques of nanoparticles preparation. Most attention is given to the various types of green synthesis, which constitute an environmentally friendly alternative to industrially applied methods. The second part deals with the preparation of noble metal nanoparticles using tea broths. The section describes the entire synthesis process and includes photographic documentation. A total of eight colloidal solutions were prepared, four of each metal. The nanoparticles were subsequently analyzed using UV-vis spectroscopy. A time-lapse spectroscopic description of each synthesis was also undertaken. Furthermore, the size distribution of the particles by volume was measured and compared with the pictures obtained by scanning electron microscopy. The results have showed that tea broths, especially those from black tea and rooibos, are suitable for the biosynthesis of stable noble metal nanoparticles.
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Gel aprotic electrolytes with tetraalkylamonium salts
Michalec, Juraj ; Veselkova, Iuliia (referee) ; Sedlaříková, Marie (advisor)
The essence of this graduate thesis is to summarize knowledge about the aprotic gel electrolytes. In the graduate thesis, there are explained methods for mensuration electrochemical properties of the aprotic gel electrolytes. In the theoretical part, I focus on the knowledge related to gel polymeric electrolytes, their history, properties, mechanisms and application. In the experimental part, I describe the preparation of the samples of the gel polymeric electrolytes, in which I evaluate their properties, electrical conductivity and potencial window.
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Optical setup for torque detected electron spin resonance spectroscopy
Kern, Michal ; Vetushka, Aliaksei (referee) ; Neugebauer, Petr (advisor)
Táto diplomová práca sa venuje vylepšeniu spektroskopu Torzne Detegovanej Elektrónovej Spinovej Rezonancie (TDESR) výmenou aktuálnej kapacitnej detekcie výchylky ohybného ramienka za optické metódy. Práca popisuje základy Elektrónovej Spinovej Rezonančnej (ESR) spektroskopie s dôrazom na TDESR a tému magnetizmu jednomolekulových magnetov. Následne je vysvetlená detekcia výchylky ramienka pomocou odrazu laserového zväzku a interferometrie. Všetky kroky nutné k skonštruovaniu spektrometra a jeho uvedenia do prevádzky sú podrobne popísané. Pomocou detekcie odrazu laserového zväzku sme úspešne získali vysoko kvalitné TDESR spektrá kryštálu jednomolekulového magnetu Fe4. Týmto meraním sme dokázali vhodnosť použitia tejto metódy a jej výraznú prevahu nad pôvodnou kapacitnou detekciou, najmä v oblasti kvality, rozlíšenia a rýchlosti. Zároveň sme na ďaľšie vylepšenie TDESR spektrometra navrhli a zostrojili zostavu využívajúcu na detekciu výchylky interferometer.
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Biodegradable bone implants based on iron
Müller, Petr ; Kazda, Tomáš (referee) ; Sedlaříková, Marie (advisor)
The present work deals with the comparison of the properties of metallic biomaterials in terms of their suitability for use as a temporary metal implant. In the work is judged biocompatibility of materials, they are comparing the corrosion rates and the influence of additives in the iron alloy to change biocompatibility and corrosion rate. In a part of this work is suggesting a method of preparing biodegradable metallic samples with different alloying elements and determine the methods, processes and measuring the corrosion rates. Part of this work is the chapter dealing with the function and effect of iron in the human body and any complications that may occur when a surplus caused by the release of part of the implant during its degradation or corrosion products. The outcome of this work is sort of created an iron-based samples in terms of their electrochemical corrosion potential, corrosion rate of samples exposed in various corrosive solutions, spectroscopic elemental analysis and outputs from the microscopic observation of the structures.
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