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Anti-aging cosmetics - latest activ substances of natural origin
Fleková, Andrea ; Vítová, Eva (referee) ; Souralová Popelková, Miriam (advisor)
The present diploma thesis is focused on the selected anti-aging substances in cosmetic products. The literature research provided us with the knowledge of essential skin characteristics and factors influencing the aging process. Furthermore, the thesis describes the basic physical forms of cosmetic products, the basic cosmetic materials and the current trends in the usage of anti-aging substances. The experimental part subsumes altogether four physical forms of cosmetic products – tonics, serums, masks and creams. In this work was testid a new anti-aging compound, developer by French company SOLABIA, which provided the company Miša a Harašta s.r.o. (M+H). It has tested four components: Fucogel, Camaderm Gly, Viniderm and Omega Ceramid. These anti-aging substances were addend to the four technology cosmetic form, which were prepared in our laboratory. Subsequently, the pH stability of the products was measured together with the rheological measuring and skin hydration measuring. All of the samples proved to be pH stable. The highest stability of viscosity and consistence was identified for masks and creams. It was found out that the added anti-aging substances have no impact on the stability of pH, viscosity and consistency. The skin hydration measuring proved that the anti-aging substances Vinidem and Camadem Gly have the greatest moisturising qualities.
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Methods of evaluation of selected cosmetics
Sládková, Veronika ; Sýsová, Lenka (referee) ; Zemanová, Jana (advisor)
This thesis is devided into two parts. The aim of the first – theoretical – part was to become acquainted with the composition, the importance and the physiology of the skin, with different types and masks in connection with the skin care. The theoretical part deals with the function of particular ingredients contained in selected cosmetics described in research literature and also with methods from which cosmetics can be evaluated. Olive oil is the most described component because it is an important ingredient for all cosmetics this study deals with. The second – experimental – part is focused on evaluating the influence of hydratation of these cosmetics. Hydratation was measured by Courage & Khazaka MPA 5 device, which is based on the capacitance measurement of the condenser. Then, the methodic evaluation of hydratation was made up and the aim of the experimental part was to proove this methodic´s accuracy and to elaborate acquired results.
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Bioactive peptides as a component of anti-aging cosmetics
Jatzová, Katarína ; Obruča, Stanislav (referee) ; Muthný,, Tomáš (advisor)
Ageing is a natural part of every human life cycle. During ageing there are lots of changes in the organism. One of the main pillars of the cosmetics industry is the development of active compounds that are fighting signs of skin ageing. The components as bioactive peptides are considered to be promising anti-ageing products, mainly because of the possibility to precisely define their chemical structure and therefore achieve more effective biological targeting. One of the signs of skin ageing is the weakening of the connections between epidermal cells and the extracellular matrix, decreasing expression of adhesion molecules and molecular components of dermo-epidermal connection. The most abundant adhesive receptors in the skin are integrins. Their ligands are extracellular matrix molecules, e. g. laminin or fibronectin. The minimal recognition sequence of integrins receptors is the amino acid motif arginine- glycine- aspartic acid (RGD). This sequence is also very interesting in terms of cosmetic applications because it provides the ability to create new and effective bioactive peptides. The subject of present work is basic safety testing of four peptides with RGD motif. A sequence of three peptides was modified by addition of glycine amino acids, or alanine. The expected effect was to improve interaction with integrin receptors. In each of the sample, levels of endotoxin was determined in order to exclude any possible interfering effects on the viability of cells. Subsequently, in NIH3T3 mouse fibroblasts viability was monitored by MTT assay and morphology. The quantity of obtained protein had been determined to increase data interpretation relevance.
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Measurement of thickness of skin and subcutaneous tissue in B-mode ultrasound image
Moldříková, Zuzana ; Štohanzlová, Petra (referee) ; Šrámek, Jaromír (advisor)
In the first part this bachelor thesis deals with theoretical analysis of terms, which are needed for understanding and algorithm proposition for skin thickness and subcutaneous tissue direct measurement. Second part of this thesis deals with implementation of algorithm. In six chapters there are described terms like skin and subcutaneous tissue, adiposity, Body Composition Analysis, ultrasound, B-mode and basics of image processing, which are going to be needed for processing of ultrasound image and algorithm creation. Chapter seven includes description of algorithm implementation and description of its structure. There is also analyzed clinical verification of functionality on phantom and some volunteers. The chapter is concluded with evaluation and discussion of gained results.
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Encapsulation of active substances into nanofibers and possibilities of their application
Procházková, Lucie ; Pernicová, Iva (referee) ; Skoumalová, Petra (advisor)
The master thesis was based on the optimization of the production of nanofiber covers and to gaine the product for subsequent functional use. The production of nanofiber covers was made by electrospinning and forcespinning from selected materials. Polyhydroxybutyrate, gelatin, chitosan and alginate were used as starting materials. After successful optimization, these materials were enriched with active ingredients ampicillin and ibuprofen for the functionalized use of covers for more effective wound healing. The theoretical part was focused on the issue of skin, healing processes, types of wounds and nanofibers, the characterization of selected starting materials for the formation of nanofibers was also mentioned. The practical part was based on the lengthy optimization of the preparation of fiber covers and later enriched with active ingredients. Furthermore, combined covers made of different materials with contents of both active ingredients were designed. This was followed by the characterization of all prepared covers from the point of view of stability in the short and long term. The gradual release of active ingredients was determined spectrophotometrically and by hifh performance liquid chromatography. It was also important to determine the antimicrobial activity of selected active substances. At the end of all testing, combined coatings containing both active ingredients were used for safety testing with human keratinocyte cells (HaCaT). Safety testing was based on determining the viability of human cells using the MTT test, to verify the LDH test. A scratch test was also performed, a wound healing test after the application of devised combined covers.
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Liveness Detection on Finger Using Various Light Sources
Vančo, Petr ; Kanich, Ondřej (referee) ; Drahanský, Martin (advisor)
The purpose of this work was to increase quality, security and reliability in biometric systems. This work presents electric and optical characteristics of a human finger. In the first part of this work you can find a theoretical basis about biometric systems, a skin and light. The next part is devoted to electric characteristics of a human finger. The third part of this work describes design, implementation and results of testing optical characteristics of a finger. In the end you can find information about some of the improvements for the future.
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Psychogalvanic reflex
Peterová, Soňa ; Harabiš, Vratislav (referee) ; Chmelař, Milan (advisor)
This thesis deals with the description, origin and possibilities of measurement psychogalvanic reflex. Also included will be the proces to measure the phenomen using either AC or DC. Psychogalvanic reflex is also associated with supraliminal stimulus. Activation or deactivation of sweat glands, controlled by free will of man, are responsive to this initiative. Attention is also given the characteristics of the electric current on human beings and the associated security scan on a living subject.
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Study of penetration of selected substances from elderberry extracts through biological membrane
Škubalová, Zuzana ; Němcová, Andrea (referee) ; Vespalcová, Milena (advisor)
This Diploma thesis focuses on the study of penetration of selected phenolic substances contained in extract from black elder (Sambucus nigra) via modelling systems represented by two types of synthetic membranes and a real system represented by a biological membrane. As a biological membrane, we have chosen skin from pig´s earlobe. Experiments of transdermal absorption and absorption through synthetic membranes were conducted through vertical (Franz´s) cells. Selected substances contained in the extract from black elder were rutin and chlorogenic acid. The main focus of this work was to determine how readily, or if at all, these two selected phenolic substances penetrate the skin and selected synthetic membranes. The percentage of absorbed amount of the substance was determined by HPLC analysis.
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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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