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Analysis of biotic matrices by Laser-Induced Breakdown Spectroscopy
Střítežská, Sára ; Karel,, Novotný (referee) ; Modlitbová, Pavlína (advisor)
This bachelor thesis discusses the possibility of using Laser-induced breakdown spectroscopy (LIBS) for biological application. The LIBS method was used to determine spatial distribution of a chosen element, cadmium, in selected model plant, white mustard (Sinapis Alba L.) The exposure took place in hydroponic conditions and lasted for 72 hours, therefore it was an acute toxicity test. Three different sources of cadmium were tested, mercaptopropionic acid stabilized quantum dots (CdTe QDs), silica coated quantum dots (CdTe/SiO2 QDs), and cadmium chloride, which was used as a positive control. After the exposure and before determination of the spatial distribution of cadmium in plants, a significant portion of the thesis was devoted to optimization of selected parameters of LIBS measurement (laser energy and gate delay). Subsequently, the cadmium distribution was determined and the obtained results were discussed with regards to the tested substance, its stability and its tendency to aggregate/agglomerate in water medium in contact with testing organism during exposure. In the final part of the work, the suitability of the LIBS method for the determination of spatial distribution of chosen contaminants was evaluated.
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Nanostructures and Nanosensors
Křečková, Jitka ; Haupt, Jaromír (referee) ; Ellederová, Eva (advisor)
Cílem této bakalářské práce je představit využití nanotechnologie ve snímacích zařízeních, poskytnout základní informace o nanotechnologii, včetně popisu a klasifikace nanostruktur, a zdůraznit možnost vylepšení výkonu senzoru prostřednictvím nanotechnologie. Tato práce se také zabývá využitím nanodrátu jako součástí senzoru s konfigurací unipolárního tranzistoru. Kvantové tečky a jejich využití v optické detekci jsou také zahrnuty v této práci. Výhody i současná omezení nanosenzorů jsou shrnuty v závěru práce.
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Biomolecules influence on optical properties of deposited quantum dots
Roček, Vojtěch ; Mravec, Filip (referee) ; Drbohlavová, Jana (advisor)
This thesis is focused on creation of quantum dots of titanium dioxide with help of nanoporous alumina mask and on funcionalization of their surface in purpose to increase the quantum yield of its. This thesis is devided into 6 main chapters, where the glossary topic of quantum dots creation process and ways of practical usage of these quantum dots are made. The theoretical knowledge is used for creation of titanium dioxide quantum dots, which was deposited by gold and funcionalized with glutation. The results are shown and discussed in the end of this work.
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Biosensors based on modified TiO2 nanoparticles
Valkovičová, Jiřina ; Adam, Vojtěch (referee) ; Hubálek, Jaromír (advisor)
The work is focused on quantum dots (QDs) array fabrication from titanium dioxide using template based non-lithographic method and their luminescence (fluorescence) properties study. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.
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Study of influence of quantum dots to biological systmes and their components
Koudelková, Zuzana ; Zítka, Ondřej (referee) ; Provazník, Ivo (advisor)
The aim of this thesis is to summarize the available evidence about quantum dots and their effects on living systems. The text describes methods for the preparation of quantum dots with respect to their characteristics (size, fluorescence wavelength) and methods of quantum dots bio-functionalization of biomolecules. In living organisms is a large number of proteins, because these are considered as one of the main components of the interaction of organisms. Therefore, the work also provides basic information about proteins. Finally, there are described various methods by which the quantum dots may be characterized mainly by differential pulse voltammetry measurement zeta potential and fluorescence measurement. The main objective of this work is to propose models of different environments in which will be degradation of quantum dots with regard to the evaluation of acquired kinetic parameters for predicting the stability of individual quantum dots.
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Electrochemical preparation of fluorescence sensor with highly ordered nanostructured surface
Bedlek, Marek ; Kolíbal, Miroslav (referee) ; Drbohlavová, Jana (advisor)
This work deals with fabrication and examination of optical properties of self-ordered TiO2 and Au quantum dots array and its potential usage in medicine as optical sensor for biomolecules detection. Controlled anodization of Ti through Al2O3 template was chosen as a fabrication method for TiO2 QDs, in case of Au galvanic deposition through Al2O3 template onto beforehand prepared TiO2 QDs or substrate with dimple structure. The influence of anodization and deposition conditions on the final structure of QDs and their emission spectrum is also examined.
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Study of UV-generated fluorescent zinc complexes by fluorescence spectroscopy
Havlíková, Martina ; Vaculovičová, Markéta (referee) ; Mravec, Filip (advisor)
This thesis focuses on the study of UV light-generated zinc complexes with cadmium and organic molecules SAM, SAH, CYS, HCYS and GSSG, specifically at 375 nm. Furthemore, the aim of the work is to characterize the precursors spectrally and temporally before and after irradiation in the transilluminator at 250 nm. Study of genesis these complexes was performed by FLIM. Thanks to this method, it was found that the formation of complexes occurs only with Zn:SAH, Zn:GSSG and Zn:Cd. The formation of complexes is influenced by the method of preparation. The spectral characteristic was performed on a fluorimeter where the increase in fluorescence intensity of the irradiated solution with the precursors was expected. These were turbid solutions where sedimentation of the particles was observed and the intensity of fluorescence was changed. In the Zn:SAM and Zn:CYS sample, the sedimentation increased in intensity, while in Zn:SAH and Zn:HCYS decreased. The Zn:Cd precursor solution was clear and there was no change in intensity. Zn:Cd showed the best spectral properties, while the Zn:SAM sample, whose excitation and emission maxima are very close to each other, appeared to be the worst. A sample with Zn:CYS and Zn:HCYS showed almost the same spectra and respective peak results. Based on lifetime characteristics by TCSPC, the sample with Zn:CYS, Zn:HCYS and Zn:GSSG, which showed 3 lifetimes, was best treated. Lifetime could not be unambiguously determined for SAM and SAH samples. Zn:Cd had 4 lifetimes
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Development of Instrumentation and Methodology in Proteomic and Environmental Analysis
Hezinová, Věra ; Bobáľová, Janette (referee) ; Lubal,, Přemysl (referee) ; Čáslavský, Josef (referee) ; Klepárník, Karel (advisor)
Tato práce je zaměřena jak na cílený tak na přehledný přístup ve studiu proteomiky. Cílená proteomika přináší informace o přítomnosti proteinu a jeho lokalizaci v buňce či tkáni pomocí luminiscenčních značek na bázi kvantových teček, zatímco přehledná proteomika se zabývá identifikací změn v proteomu dvou nebo více jedinců stejného druhu vystavených různým podmínkám. Protože proteomika vyžaduje vysoce citlivé separační a identifikační techniky, byly v této práci ověřeny různé metody zlepšení citlivosti kapilární elektroforézy s hmotnostní detekcí. Použití rozhraní s kapalinovým spojem pro spojení těchto dvou technik, které zajišťuje vyšší citlivost analýz, bylo také ověřeno analýzou metabolitů etanolu a kokainu v lidské moči. Zavedené techniky instrumentace jsou využitelné při posouzení vlivu významných faktorů životního prostředí na živé systémy jak na buněčné tak na molekulární úrovni.
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Study of metallothionein conjugates by electromigration methods
Johaníková, Klára ; Zemánková, Kristýna (referee) ; Vaculovičová, Markéta (advisor)
The bachelor thesis deals with the study of conjugates of metallothionein (MT) with fluorescent nanoparticles using electromigration methods. Particular attention is paid to the preparation and characterization of quantum dots (QDs) and their bioconjugates with the metallothionein protein, whose separation conditions (concentration and suitable electrolyte pH) have been carefully optimized. For the preparation of QDs, the action of UV radiation for one type of quantum dots and the thermal action of high temperature for the second type of species were used. Conjugation of QDs to the protein was performed via a carbodiimide-zero-length crosslinker (EDC / sulfo-NHS), which activates carboxyl groups and allows bioconjugation of the ligand by covalent bonding. The resulting bioconjugates were studied by capillary electrophoresis with absorption detection ( 214).
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