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Effect of cancer therapy on fertilization parameters in testicular germ cell tumour patients.
Kruf, Annabelle Elisabeth ; Vondráková, Jana (advisor) ; Nevoral, Jan (referee)
Testicular germ cell tumours (TGCTs) are a rare type of cancer. It is the most common type of cancer in the age group of 15-35 years. The consequences that TGCT and the treatment of this disease impose to these young men in terms of sperm parameters and fertility is yet unclear. This study involves 84 semen samples from 46 patients sorted in three time groups - before treatment, 3 months after the treatment and 6 months after the treatment. Several sperm parameters were studied and compared within the three time points. Adding on to this, the study takes two patients with complete set of semen samples and studies them individually. The results mostly show great differences in patients and this study underlines the individual characteristics that need to be taken into consideration when it comes to patients' fertility journeys after battling TGCT. Sperm viability, concentration, apoptosis, DNA damage, acrosomal integrity and leukocyte concentration nor histone modification evaluation showed any significant changes between said groups. DNA damage was correlated to the level of acrosomal permeability. IgA antibody levels rose significantly in samples after orchiectomy. IgG antibody levels did not show the same trend. A novel approach was optimized and applied using JUNO and FcRL3 double transfected...
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Changes in fertility characteristics and population structures in Southern and Eastern Europe: interrelationships and contexts
Kahoun, Lukáš ; Šídlo, Luděk (advisor) ; Kurtinová, Olga (referee)
Changes in fertility characteristics and population structures in Southern and Eastern Europe: interrelationships and contexts Abstract There were significant changes in fertility characteristics and population structures between 1970 and 2020 in both Southern and Eastern Europe. This transition is associated with the concept of the second demographic transition by some authors. An unconventional methodology is used for its research in this thesis. Two three-dimensional scatter plots, which at the same time form unit cubes, are constructed using three selected indicators of both changes (fertility and structures). The distance between individual observations by examined years is interpreted as the dynamics of the transition of the given decades. The dynamics values from both scatter plots are then analyzed and compared with the others. The results show that changes between fertility characteristics and population structures are related to a certain extent, but the observed differences between regions or the specifics of some states complicate the generalization of this relationship. These conclusions also agree with the literature published so far. Key words: fertility, population structures, second demographic transition, data visualization, three-dimensional scatter plot Character count: 149 752
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Estimating willingness to pay for increasing probability to conceive
Kolářová, Natálie ; Ščasný, Milan (advisor) ; Šťastná, Lenka (referee)
The purpose of the thesis is to analyze preferences for increasing the probability of conceiving. Willingness to pay for increasing the probability of conceiving is estimated using the private good scenario and a new complex of vitamins and minerals as the contingent product. Specifically, we use the discrete choice experiment with three attributes: the increase in the probability of conceiving, the number of months of trying to conceive from when this probability will be increased, and the cost to be paid for this product. Data are obtained through a stated preference survey conducted in 2014 in four European countries (the Czech Republic, the United Kingdom, Italy, and the Netherlands). We estimate a mixed logit model, allowing for unobserved preference heterogeneity. We find that people are willing to pay to increase a female's probability of conceiving, and their willingness to pay is higher after 12 months of trying to conceive when one is becoming infertile. Respondents are willing to pay for increasing the chance to conceive even during the period when they are not diagnosed as infertile, i.e., from 6 months of trying to conceive, except in the Netherlands. Italians are willing to pay the most, while Dutch are willing to pay the least. By controlling for the main characteristics of...
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Influence of avian Plasmodium on their hosts
Dulavová, Kristýna ; Svobodová, Milena (advisor) ; Vinkler, Michal (referee)
Avian malaria parasites of the genus Plasmodium are unicellular (and intracellular) protozoans of the group Haemospororida, using a blood-sucking insect (mosquito) as a vector and a bird as a vertebrate host. The effects of this disease, together with avian pox, have greatly influenced endemic populations of Hawaiian birds after introduction of a suitable vector to the islands by a human hand, leading to a drastic extinction of bird species; most of the remaining Hawaiian endemic species are now endangered. Apart from that, avian malaria is distributed nearly all over the world, infecting a variety of different hosts. This thesis is focused on the influence of avian malaria parasites mainly on their avian hosts. The most severe patologies caused by Plasmodium are associated with blood stages causing serious anemia in birds. More pathologies are connected with exoerythrocytic stages in the endothelial cells in various organs and are accompanied with external signs such as lethargy, respiratory distress, weakness, inappetence, sometimes weight loss and so on; different plasmodium species-bird host combinations have different outcomes. An interesting issue is the emerging tolerance against avian malaria in Hawaii Amakihi (Hemignathus virens), a species occupying low elevation areas of Hawaiian...
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Population development of district towns in the South Bohemian Region
Votavová, Kateřina ; Šídlo, Luděk (advisor) ; Brázová, Kateřina (referee)
Population development of district towns in the South Bohemian Region Abstract The aim of the thesis is to analyse and describe the population development in the seven largest cities of the South Bohemia Region in the period 1997-2021. The thesis draws on individual and anonymised data to analyse the basic demographic indicators of fertility, mortality and migration. The work compares the cities with each other as well as compares the development with the national average. Among other things, the thesis examines the development documents of cities, especially their efforts to address demographic issues. Moreover, the analysis shows that most cities, with the exception of Písek, tend to lose population, but with different intensity and timing. In all cities fertility intensity and mortality evolve similarly with minimal differences. Finally, the city management is currently trying to address demographic issues in most cities. Keywords: population development, towns, fertility, mortality, migration
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Fertility of women aged 35 and over in selected European countries
Zetová, Eva-Marie ; Šťastná, Anna (advisor) ; Waldaufová, Eva (referee)
Fertility of women aged 35 and over in selected European countries ABSTRACT This bachelor's thesis deals with the issue of fertility postponement in Europe. It focuses on the fertility of woman aged 35 and over of age in the Czech Republic, the Netherlands, Spain and Norway between 2010 and 2020. It describes the postponement of fertility in a historical context, influencing factors, risks associated with women's fertility at the age of 35+, childlessness, and fertility differentiation by age. The method of analysis is fertility demographic analysis from a transversal perspective. The thesis examines fertility trends of women aged 35 and over, as well as the current similarities and differences in the fertility of older women in the studied countries. Data analysis showed that in all studied countries and years, the proportion of the fertility intensity of women aged 35 and over to the total fertility rate is increasing. Keywords: fertility, fertility postponement, late motherhood, demographic analysis
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Analysis of reproductive indicators in dairy cow breeding
KOCOUR, Daniel
The aim of the diploma thesis was to evaluate the trends in the development of reproductive indicators in specific dairy farming and to define the possible causes of the identified problems and propose measures to eliminate or resolve them. The literature review deals with the physiology of cow fertility, synchronization of estrus, hormonal or nervous control of the sexual cycle, the effect of internal and external influences on the reproduction of dairy cows, the effect of reproduction on milk yield and the economic connections associated with the reproduction of dairy cows. The analysis was carried out in the herd of Czech spotted cattle ZD Lukavec in the district of Pelhřimov. A total of 120 dairy cows that ended lactation between January 1, 2022 and December 31, 2022 were included in the monitoring. There were 40 dairy cows in the 1st lactation, 40 dairy cows in the 2nd lactation, and 40 dairy cows in the 3rd and 3rd lactations. higher lactation. In order to evaluate the effects on reproductive indicators (interval, insemination index, length of service period and intermediate period), the data set was sorted by age at first calving, parity, calving and insemination season, milk yield at laktation peak, length of the previous calving interval, RBV of sire and inseminated bulls, occurrence of mastitis and other diseases. The best reproductive results were found in dairy cows that calved for the first time between 715 and 790 days of ageThe length of the service period was the shortest among primiparous heifers. However, the order of lactation had no significant effect on the insemination index. Cows calved in the summer months (June 21, 2021 to September 23, 2021) clearly had the worst reproduction indicators. The time of year when the 1st insemination was performed, however, did not significantly affect reproductive indicators. The length of the previous interval significantly influenced only the length of the insemination interval. Cows with the lowest daily milk yield at peak lactation had the shortest service period and intermediate period. But they also had the lowest average lactation rank. The influence of the RPH of the father on the fertility of the daughters was evident only for the length of the previous intermediate period, when the daughters of bulls with the highest RPH (?106) had the shortest intermediate period (386 days). However, reproductive indicators were significantly influenced by the RPH of the inseminated bull for its own fertility.
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Umělý výtěr karase obecného
VRBENSKÝ, Jakub
The thesis briefly summarizes the available information in the field of crucian carp biology. The experimental part includes, firstly, monitoring the effect of water temperature during the latency period in reproductive females affects the success of artificial spawning, the survival of eggs to the eyespot stage, the length of the latency interval in hours and in clock degrees, the relative fecundity of reproductive females and the pGSI. In the second part of the experiment, secondly, the effect of the method of unstickness eggs on the survival of eggs to the eyespot stage. To test the effect of temperature during the latency interval, the females (mean weight 329 +- 60 g) were divided into 7 groups (n = 5). The individual groups of reproductive females were placed in separate tanks with average water temperatures of 15.4, 17.2, 18.9, 21, 23.2, 25.2 and 27.2 °C. Females and males were injected with the hormone Ovopel (1 pellet per 1 kg of fish). Before injection and artificial spawning, the reproductive fish were anaesthetized with clove oil (0.03 ml.l-1). The each group of females were artificially spawned, the eggs were seeded with a mixture of sperm from three males, activated with hatchery water and unsticked with cow's milk diluted with water (1:9). Incubation of eggs was carried out in small experimental Zug bottles (0.45 l) at an average temperature of 21 °C. The highest number of ovulated and artificially spawned females was found at temperatures of 21 - 25 °C (80 %). At lower and higher temperatures the success of spawning was lower. A close positive linear dependence of the latency interval length (in hours) on water temperature was found in the monitored range of 15.5 - 27.2 °C (y =-1.5062 x + 50.663; R2 = 0.924). The length of the latency interval at 21 and 23 °C was 18,4 +- 0,9 and 12,4 +- 0,2 h, respectively 387 +- 18 and 289 +- 5 h°. The eggs have a yellow-brown to green-brown colouration, the average weight of one unswollen eggs is 1,10 +- 0,07 mg (corresponding to 911 +- 51 thousand eggs in 1 kg of eggs). In contact with water, the eggs are very sticky. The highest relative working fecundity was achieved at 21 and 23 °C (71,9 +- 10 and 75,9 +- 34 thousand eggs per 1 kg of female). The average survival rate of eggs to the eyespot, originating from artificially spawned females kept during the latency interval at temperatures between 18,9 - 23,2 °C, ranged from 92.9 +- 0.5 to 99.6 +- 2.2 %, and was lower at 17.2 and 27.2 °C (86.2 +- 0.6 respectively 74.2 +- 3 %), differences between groups were statistically significant. In evaluating the effect of unsticking preparations on the survival of eggs to the eyespots, the highest survival was achieved with milk (71.9 +- 7.3 %). Statistically conclusively lower survival was found with tannin (47.4 +- 0.4 %) and acetylcysteine (48.4 +- 8.6 %). The achieved results can be used in artificial spawning of crucian carp as part of rescue breeding.
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Midwife and Reproductive Health
KUBIŠOVÁ, Kristýna
The bachelor thesis focuses on reproductive health with the coherence of midwife's work. The thesis is divided into theoretical and practical parts. The theoretical part deals with the definition of reproductive health, the physiology of human reproduction, the menstrual cycle, and the need for sex and reproductive education. Furthermore, the function of the public health service system is explained as well as the prevention as part of primary care in the Czech Republic. The midwife's doing is clarified along with her competence and her meaning in the prevention topic. The factors which influence reproductive health such as age, diet, recreational drug use, stress, etc. Are described in detail. The bachelor thesis also centers on family planning, a healthy conception, and types of contraceptive methods of birth control and some information about infertility is mentioned as well. The practical part aimed to discover upper-secondary and tertiary education students' opinions about reproductive health. Further, two hypotheses were determined, which were formulated as follows. Hypothesis n. 1: The tertiary students perceive the need for reproductive health education from a midwife more than upper-secondary students. Hypothesis n. 2: Women perceive the need for reproductive health education more than men. Qualitative data collection was chosen on which the questionnaire was based. The questionnaire contained 41 questions (31 closed, 8 mid-closed, and 2 open ones). It was spread in online form through Google Forms application. The research aggregate consisted of 210 respondents from upper-secondary education students (3rd and 4th years) and tertiary students from various occupations. The research sample was composed of students between the age of 17 to 27 from numerous regions of the Czech Republic. Fisher exact test was used for data analyses of the formulated hypotesis. The level of significance was stated as follows alpha = 5 % (0.05). H1 was not confirmed. It was proven that interest in reproductive health education is not dependent on students´ education; either upper secondary or tercially education degree. Also, it was not confirmed that the reproductive health education is required to be performed by midwife. H2 was confirmed. Based on the analysis was proven that women require reproductive health education to be performed by midwife more than men. According to 87 % (182) respondents of our survey the reproductive health education should be included in upper-secondary and tertiary education but 5 % (11) of respondents would not include it at all and 8 % (17) was not sure. The survey has shown that the ideal learner should be an externally educated person in reproductive health. This person would suit 78.5 % (124) of respondents, 8.2 % (13) of students would like to have a doctor for education in this topic. However, 6,3 % (9) of students would like to have teacher from their school and only for 5.1 % (9) of students the midwife would be suitable. The rest of the students, 1.9 % (3) would like to have somebody else. The Bachelor thesis and the survey could be used for purpose of educational plans and materials in topic of reproductive health education for students.
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Porovnání vývoje plodnosti a sňatečnosti v České republice a v Irsku
Blechová, Veronika
The subject of this bachelor thesis is development of fertility rate and marriage in the Czech Republic and Ireland between years 1989 and 2012. The theoretical part generally describes terms as fertility rate, marriage, their basic indicators, factors that influence these demographic processes and historical development of fertility rate and marriage in both countries. Last chapter of the theoretical part gives basic explanation of the time series. Practical part includes comparison of development of fertility rate and marriage, which is implemented by using time series based on the alignment of the trend line and using the average growth rate in the Czech Republic and in Ireland.
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