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The use of high-performance separation techniques for monitoring the bioaccumulation of selected micropollutants in plants of model root treatment plants
Modroczká, Karin ; Sobotníková, Jana (advisor) ; Coufal, Pavel (referee)
This bachelor's thesis deals with the determination of ibuprofen in model root refineries from Phalaris arundinacea extracts. The goal was to find out if the plant is able to accumulate ibuprofen from the aqueous solution which was added to the plant. A HPLC method with UV/VIS detection was also optimized to determine the ibuprofen content. A C18 column (4.6 mm × 150 mm × 3.5 µm) was used to analyse the samples. An isocratic program was used, in which the mobile phase consisted of methanol and an aqueous acetic acid solution of pH = 3,27. As part of the validation of the method, the repeatability, robustness, and yield (which was 99,04 %) of the method were verified. Furthermore, the linearity of the method, which was from 0,01 mg ml-1 to 2,0 mg ml-1 , the limit of detection (LOD = 0,049 mg ml-1 ) and the limit of quantification (LOQ = 0,16 mg ml-1 ) were determined. After the validation of the method, we can claim that the method is accurate, because the values of the relative standard deviations did not exceed 2 %. Infrared spectroscopy was used to verify the purity of ibuprofen batches. Last but not least, it was proven that Phalaris arundinacea has the ability to accumulate ibuprofen from an aqueous solution. Keywords: HPLC, ibuprofen, Phalaris arundinacea, root wastewater treatment plant,...
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Natural wastewater treatment plant project for the municipality of Hačky
Kulichová, Jana ; Pumprlová Němcová, Miroslava (referee) ; Kriška-Dunajský, Michal (advisor)
The aim of the thesis is research, description, and subsequent calculation and design of the natural wastewater treatment plant. The conclusion is the processing of project documentation. The thesis is divided into the theoretical and practical parts. In the theoretical part, the aim is a general description of the location and research of natural wastewater treatment plant technologies for small municipalities. In the practical part, there is designed project documentation, which will be used as an input basis for zoning management and building permit.
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Project of a natural wastewater treatment plant for the municipality of Oponešice
Kraváček, Václav ; Zedník, Ondřej (referee) ; Kriška-Dunajský, Michal (advisor)
The diploma thesis deals with the design of a natural wastewater treatment plant for the municipality of Oponešice. The introduction of the thesis focuses on general research presenting the issue of natural wastewater treatment plants (WTP). Except for a short introduction of natural treatment plants, the related legislative regulations are presented in the introductory part, the work is devoted in more detail to the presentation of individual monitored indicators of wastewater pollution and a description of individual technological elements at root treatment plants. The second and main part of the work is the complete design of the wastewater treatment plant for the village of Oponešice. First, attention is paid to collecting information about the given locality. Next follows the design of the technical solution of the treatment plant itself, in which care was taken to meet all the requirements placed on treated wastewater, with an effort to design the best available technology for the municipality of Oponešice. Within individual objects, input parameters and subsequent calculations are always given, accompanied by a description of individual details. As the main stage of cleaning, a two-stage vertical system of the so-called Austrian type, supplemented by pre-cleaning with the help of an anaerobic separator, is proposed. At the end of the work, an evaluation of the overall efficiency of the proposed technology is described and a confrontation with the applicable legislation is carried out. Last but not least, an important part of the work includes the preparation of complete project documentation (drawings and textual parts), which is to serve as input material for the issuance of zoning regulations and building permits.
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Natural wastewater treatment plant project for the municipality of Skřipov
Hubený, Michal ; Pumprlová Němcová, Miroslava (referee) ; Kriška-Dunajský, Michal (advisor)
The diploma thesis deals with the substantive design of a natural wastewater treatment plant of the French system for the municipality of Skřipov. The proposed sewage treatment plant has an expected size for 800 EO. The whole thesis is divided into two parts. In the theoretical part, the details of the area under consideration, especially the municipality of Skřipov, are presented and thoroughly described. Furthermore, a general research on the topic of natural wastewater treatment plants was written by the author of the thesis. In the theoretical part of the thesis there is also a detailed description and introduction to the system of French vertical wastewater treatment plants. The practical part is the design of a natural wastewater treatment plant for the municipality of Skřipov. The thesis describes in detail the design procedure of the treatment plant and its calculations. The work includes project documentation that will serve as a basis for the issuance of a building permit.
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Evaluation of the efficiency of root treatment plants via ecotoxicity tests
Konečná, Kateřina ; Procházková, Petra (referee) ; Zlámalová Gargošová, Helena (advisor)
This bachelor thesis focuses on the evaluation of the efficiency of root treatment plants using appropriately selected ecotoxicity tests. For these purposes, ecotoxicological tests in an aquatic arrangement were used, specifically with the planktonic organism Thamnocephalus platyurus and the plant Lemna minor. The evaluation was performed at a municipal root treatment plant, which treats water for 955 equivalent inhabitants. The results of tests with T. platyurus confirmed that the root treatment plant sufficiently relieves acute toxicity of water. LC50 values were determined for two samples, 213.71 ml / l for the inflow and 388.20 ml / l for the settling tank. At the outflow, these values could no longer be determined due to the low ecotoxicological effect of the water thus treated. For tests with L. minor, IC50 values were also calculated for the first two samples. For Sample No. 1, the IC50 value was calculated to be 311.25 ml / l, for Sample No. 2 to 963.35 ml / l. However, on the basis of tests of direct toxicity of waters with L. minor, it is possible to confirm even in the case of this organism, a good cleansing effect of this root treatment plant.
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Study of variants of sewerage of the recreational center Vřesovice
Krulová, Martina ; Frajt, Tomáš (referee) ; Hluštík, Petr (advisor)
The diploma thesis deals with the possibilities of sewerage and wastewater disposal in the recreational centre near Vřesovice. The purpose of the diploma thesis is the elaboration of variant solutions of sewerage, including the proposal of possibilities of wastewater treatment, economic evaluation of variants, their subsequent comparison and recommendation of the most suitable variant. The diploma thesis deals with the design of variants of wastewater treatment with subsequent discharge into surface waters. Finally, the advantages and disadvantages of the individual variants of sewerage are summarized with a comparison of costs and subsequent recommendation of the variant.
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All-embracing water management in a residential object in a Pařezov village
Királ, Martin ; Hubačíková,, Věra (referee) ; Hyánková, Eva (advisor)
This diploma thesis deals with sound water management and is divided into theoretical and practical part. The theoretical part is divided into three main chapters. The first part focuses on rainwater, its quality characteristics and utilization. The second chapter focuses on the management of waste water, its properties, the amount of waste water and natural ways of wastewater treatment. The last chapter is devoted to the gray waters and their utilization. The practical part focuses on the study of the draft comprehensive management of sewage and rainwater in the village Pařezov district Domažlice. It focuses on wastewater treatment using constructed wetlands, using rainwater for irrigation and infiltration of the remaining water.
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Problematika vypouštění odpadních vod z malých obcí do vod povrchových
Dlouhá, Eva
The diploma thesis is facused on the wastewater discharged into surface waters in the village Nová Ves u Leštiny. Comparison parameters were with respect of flasitibility and economical demands. The suggested variants include a central wastewater treatment plant with a gravity separate sewer system or pressure sewage transport. Central root wastewater with a gravity separate sewer system or pressure sewago transport. Village will provide one-time financial contribution to individual households for acquiring domestic wastewater treatment plant.
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Study of the water management in a family house with an application of natural wastewater treatment
Kiszová, Radka ; Kriška-Dunajský, Michal (referee) ; Hyánková, Eva (advisor)
Bachelor thesis focuses on water management in the family house using natural treatment of waste water. Text partially deals with research in the area of treatment and reusing of rain and waste water in the case of a family house. Thesis concentrates on sources of waste water, their division and possibilities of reuse. When talking about waste water, main aim is to explain root waste water treatment. Second half of the text presents complex solution of water management in one particular family house. The actual amount of waste water is calculated for number of five equivalent residents, suggestion of its treatment, accumulation and enumeration of presumed amount of rain water captured. Shared storage is designed for both waste water treatment and rain water. This storage is subsequently used for flushing, washing and garden irrigation.
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