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Physiological basis of extended longevity in termite kings and queens - is activation of telomerase mechanism linked with extended longevity of termites?
Pangrácová, Marie ; Hanus, Robert (advisor) ; Kodrík, Dalibor (referee)
- Extended longevity and high fecundity are two phenomena typical for reproductive castes (queens, eventually kings) of eusocial insects. In my thesis, we explore the hypothesis that the longevity of reproductives in the termite Prorhinotermes simplex is linked with the activation of the telomerase enzyme complex. Telomerase is well known for its life-extension functions, due especially to its capacity to prolong the telomeric ends of chromosomes. Therefore, we studied here the gene expression of: (1) the gene TERT coding for the catalytic subunit of the telomerase and (2) the genes of the main endocrine regulatory pathways, known to be responsible for the control of reproduction and longevity in insects. Expression dynamics of these genes were measured in sterile and reproductive castes of P. simplex during their development and sexual maturation. Based on our results obtained from the TERT expression analyses and their comparison with telomerase enzyme activities, we assume that the telomerase action in the long-lived reproductive individuals is regulated at a post-transcription level. Furthermore, we observed in reproductive castes a simultaneous upregulation of some transcription variants of vitellogenin and the genes for insulin signalling pathways. We can, therefore, conclude that in...
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Vyhodnocení ukazatelů užitkovosti a dlouhověkosti ve vybraném stádě holštýnského skotu
BUNKOVÁ, Olga
Cattle farming is one of the most important branches of animal husbandry both in Czechia and worldwide. Cattle is the largest source of milk for human consumption in the world. Its digestibility and high contain of protein are reasons why cow's milk and dairy products have been ireplacable part of human diet for millennia. Holstein cattle is a black and white breed known for the highest milk production and great adaptability. Numerous studies have shown holstein cattle is able to adapt to many different climate conditions while keeping its high usability. Main purpose of modern holstein cattle husbandry is increase of production, shortening of calving intervals and lowering age at first calving. That is however closely connected to longevity and especially to the lenght of productive life, which are the deciding factors as the priority is to maintain cattle farming cost effective. The purpose of this work was to research factors that influence production, reproduction and longevity of holstein dairy cattle. One part is deals with observing and analysing herds of holstein cows from two different farms. Collected and analysed information about production, reproduction and life expectancy were compared using appropriate statistical methods.
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Vliv věku při 1. otelení na užitkovost a dlouhověkost dojnic
HARANTOVÁ, Barbora
In this bachelor thesis, it has been dealt with evaluating an influence from level of rearing during the first calving to the performance and longevity of dairy cows from chosen herd near VOD Svatobor. Gathered data has been filtered and ordered due to cause of exclusion, age in first calf, order of lactation and lifelong performance. There were 100 dairy cows to be observed together with their market production of milk. From this set 63 cows were from Czech mottled breed and the rest 37 cows from Holštýn's breed. Cows from both breeds has been housed in same stable and with the same aliment and treatment conditions. From results we can see that belated swallowing has little influence on the amount of produced milk. On the other hand, belated swallowed cows, so even calved cows, did not produce larger amount of milk. The only advantage of belated swallowing has been higher percentage of fat and proteins in milk. The most frequent reason of exclusion in both breeds has been illness of udder. Due to this fact cows were excluded on 1.67 lactation in average. The illness of udder may be caused by insufficient hygiene or incorrect milking procedure.
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Aktivita telomerázy u termita \kur{Prorhinotermes simplex}
JEHLÍK, Tomáš
Social insect is known for its unique caste system, coherence and effective division of labor, but also for the extreme longevity of reproductive individuals in comparison with asexual castes. Although mechanisms leading to lifespan differences between reproductive and non-reproductive castes of social insects are not sufficiently explained, one of the longevity determinants might be telomere length and activity of telomerase as the most common mechanism of telomere length maintenance. Telomere length belongs to general indicators of organismal lifespan. This work is focused on monitoring of telomerase activity in various stages, castes and organs of the termite Prorhinotermes simplex (Isoptera: Rhinotermitidae) showing up-regulation of telomerase in reproductive castes.
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Relationship between longevity and non-specific stress in the Early Medieval Moravian population
Zazvonilová, Eliška ; Brůžek, Jaroslav (advisor) ; Stránská, Petra (referee)
Opinions on the relationship between non-specific stress and age at death on adults are not uniform. The inspiration for our diploma thesis was a study by Croatian authors Becic et al. (2014) who reported that individuals with non-specific stress indicators (cribra orbitalia) lived longer. Our aims were following: to estimate the longevity and compared influence of used methods on the relationship between non-specific stress and age at death, to test the relationship between age at death and non-specific stress indicators, particularly cribra orbitalia and linear enamel hypoplasia, and also to test the relationship between non-specific stress indicators and stature. Methods for age estimation are divided into two parts, methods used in the study Becic et al. (2014) and our method selection chosen from the literature appropriate for the oldest individuals. In this study, the presence of cribra orbitalia and enamel hypoplasia was observed in 294 adult individuals (111 women, 135 men and 48 undetermined individuals) from the Early Mediaeval (9th - 10th century) Slavic skeletal series at Mikulčice (Great Moravia), IIIrd church. When comparing age at death estimated by two different technics we found reduced age at death for the individuals with cribra orbitalia and also with enamel hypoplasia. There...
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Analysis of longevity in the Czech Republic and Sweden
Hejnalová, Lucie ; Antovová, Michaela (advisor) ; Dotlačilová, Petra (referee)
The aim of this bachelor's thesis is an analysis of longevity issue in Czech Republic and Sweden from 1969 to 2015. This thesis deals with very old old people and their sex structure and summarizes data sources of them. This analysis is concerned with a historical development of longevity and a current situation. Finally is provided a predict futures value of selected indicators in 2035. The forecast is performed using trend functions. The analysis of longevity shows increasing number very old old people in both countries. Between very old old people exist more often women than men. Level longevity of Czech Republic approaches to level of Sweden. The predict shows that number of seniors and very old old people will increase in future.
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The longevity in mole rat.
Jelínková, Alena ; Schierová, Michaela (advisor) ; Španielová, Hana (referee)
Naked mole rat and blind mole rat are useful model organisms for human age-associated diaseases studies. Unlike human, their long lifespan is not accompanied by physical health impairment. In both species, the genes involved in aging process or carcinogenesis are under positive selection or their regulation differs from the regulatory pattern known in other rodents or human. Some genes are present in higher number of copies, missing or entirely new and not observed in other organisms. In naked mole rat, the degenerative development is reduced by elevated level of proteins which prevent amyloid β aggregation and contribute to oxidative damage tolerance. Their healthy aging is also caused by effective elimination of damaged proteins, natural caloric restriction or angiogenesis enhancement. High level of α-2-macroglobulin in blood, which is able to inhibit signal pathways required for tumor growth and malignancy, as well as the early contact inhibition repress tumorigenesis in naked mole rat. Many different mechanisms are involved in prolonged lifespan in both naked and blind mole rat species. The aim of this thesis is to present the most important genome and proteome differences contributing to their long lifespan. Key words: naked mole rat, blind mole rat, tumorigenesis resistance, senescence,...
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Evolutionary aspects of Alzheimer's disease. Major role of the Apolipoprotein E
Škubica, Patrik ; Daňková, Pavlína (advisor) ; Jirák, Roman (referee)
Alzheimer's disease is one of the most discussed conditions, due to its huge social and economic consequences. Whereas molecular and genetic aspects causing early onset of the disease are relatively well known, it still remains to be clearly shown how genetic risks and environmental factors interact to ultimately cause the late onset form. Major molecular-genetic factors affecting risk of developing Alzheimer's disease are APOE gene and its product apolipoprotein E. This gene occurs in humans in three common variants that differ among each other in exon sequence by one single nucleotide polymorphism. Similar difference exists between human and mammal APOE gene that served as an origin for human forms. This thesis discusses possible evolutionary scenarios of APOE gene and links acquired information to molecular and environmental ascpects of Alzheimer's disease.
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Phenomenon of longevity
Krejná, Anna ; Ondrušová, Jiřina (advisor) ; Cimrmannová, Tereza (referee)
Thesis entitled Phenomenon of longevity aims to describe and define very actual today Thema longevity. There is also mentioned particularly contemporary view of the phenomenon and its historical development. This work also touches demographics and life expectancy, which is closely linked to longevity. Great attention is paid to the problem spots in the lives of very old people, especially their disadvantaged status. On this chapter is followed also the care and support of life, that is in such high age necessary. Part of the work are also two research projects that enable an insight into the past lives of long-lived seniors in their health and functional status, and especially trying to uncover the causes of longevity.
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Vliv vybraných faktorů na dlouhověkost dojnic holštýnského skotu
HUBÁČEK, Tomáš
The aim of this thesis was the evaluation of selected factors on milking yield and longevity of the selected herd of dairy cows of Holstein cattle in the company Prima Agri a.s. There were monitored the indicators of dairy cows of Holstein cattle such as genotype, milk performance, the order of the lactation, the age of the first calving, the length of interim and of the service period, lifetime performance and reasons for elimination. The data were taken from the dairy cows whose first lactation was finished from January 1st to December 31 st 2016. 385 dairy cows were included to the monitoring. As for the genotype influence on milk performance, statistically significant difference was found out between the first and the fourth and the third and the fourth group on the level of significance (P<0,01). The effect of the age of the first calving on milk performance in kg of milk on first lactation wasn´t statistically provable (P>0,05). Statistically highly significant difference was found out by the effect of the order of the lactation on milk performance on the level of significance (P<0,01) between the groups of the first lactation and of the other groups of lactation. Statistically significant difference was found out between the 2nd lactation and the 4th and higher lactation on the level of significance (P<0,05). The effect of the genotype on the length of interim and the effect of the order of lactation on the length of interim weren´t statistically provable (P>0,05). The effect of genotype on length of the service period was statistically significant on the level of significance (P<0,01) between the group H2 and the group H3. Statistically significant difference (P<0,05) was found out between the groups H1 and H3, H1 and H4 and as well as between the groups H2 and R. The effect of the order of lactation on the length of service period was statistically highly significant on the level of significance (P<0,001) between the groups of the first lactation and of the other groups of lactation and between the groups of the second and fourth and higher lactation. The effect of the genotype of eliminated dairy cows on lifetime performance in kg of milk was statistically highly significant on the level of significance (P<0,001) between the groups H 1 and H2, H1 and H3, H2 and H3, H2 and R, H3 and R. An average number of eliminated dairy cows was 28,62 %. The biggest number of the eliminated dairy cows was on the grounds of fertility disorders and that is 35%. Longevity of the eliminated dairy cows was 2,7 lactation.
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