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The importance of variety in yield of table potatoes (\kur{Solanum tuberosum} L.)
ŠPLÍCHAL, Jonáš
In the theoretical part of the bachelor thesis, attention was paid to the description of the tuber potato, morphological characteristics of the potato, and the division of varieties according to the length of the growing season and according to the cooking type. In the following chapters, the growing principles and conditions of potato pro-duction, the content substances in potato tubers and the possibilities of their influ-ence by cultivation technology and breeding were defined. Equally important is the chapter devoted to the varietal variation of production and content parameters of potato tubers since the practical part of the bachelor thesis was devoted to this to-pic. For this purpose, a small-plot experiment was set up at the Lukavec site (620 m above sea level) with potato varieties Adéla, Antonia, and Rosara, and the yield parameters (total yield, proportion of tubers over 35 mm) and selected content cha-racteristics (starch and dry matter content) were monitored. The Rosara variety had the highest starch and dry matter content.
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Response of potatoes to split application of nitrogen and plant auxiliaries
MICHALÍČEK, David
The aim of the diploma thesis wasto evaluate the yield of tubers and the share of starch in tubers in potato varieties with different growing seasons and to point out or refute the need for nitrogen during cultivation. Next, to focus on the effect of nitrogen fertilization on dry matter and starch content. It was a small plot experiment, which was based on the Valečov experimental station. Two varieties were selected for the experiment, the early and the semi-early ones. The first variant is a plant not fertilized with nitrogen. The other variant was fertilized before planting. In another variant, a split application of nitrogen was performed, and in the fourth variant, an auxiliary plant preparation was applied, supporting stolon growth and root formation. The experiment further records treatment and conditions during the growing season. Individual differences between the varieties were also recorded during the experiment. For variants 2 and 3, the effect of fertilization on the yield of potato tubers in both varieties was demonstrated. The yield also increased significantly in the variant with the use of an auxiliary preparation. The semi-early variety Antonia reacted best to the auxiliary herbal preparation and the early variety Colomba achieved the highest yield in the variant with foliar application of urea. Antonia recorded a yield of 58.4 t/ha for the control variant, 64.3 t/ha for the second variant and 66.9 t/ha for the third fertilizer before planting. The variant with an auxiliary product then recorded a yield of 68.53 t/ha, which was a higher yield compared to the control variant by 10.1 t/ha. For the very early Colomba variety, the yields for the variant without fertilization were 66.14 t/ha, variant No. 2 then 83.18 t/ha, the variant with fertilization before planting recorded a yield of 79.26 t/ha and the variant with auxiliary product 78.85 t/ha. The differences between the fertilized variants during the growing season were 4.23 t/ha for the Antonia variety in favor of the auxiliary plant preparation. For the Colomba variety, the difference was 4.33 t/ha in favor of the urea variant. At the same time, the size ratio of the tubers also changed. In the case of the Antonia variety, there was a decrease in starch content in the fertilized variants, but in the case of the Colomba variety, the decrease was not confirmed in all variants.
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Utilization of waste banana peels for packaging materials
Vachta, Jonáš ; Mikulíková, Renata (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with isolation of cellulose from waste banana peels and it’s use as a filler in composite films. The aim of this work was to prepare a bioplastic capable of being an eco-friendly alternative to conventional polymers with a potential for application in the packaging industry. The cellulose was isolated from banana peels using a method combination of alkalic and acidic hydrolysis and bleaching by sodium chlorite. The samples after isolation were characterised by Fourier transform infrared spectroscopy (FT-IR) and their average particle size was determined by microscope observation. Subsequently, composite films were prepared using agar and starch matrix, glycerol as plasticizer and isolated cellulose as filler in various concentrations to assess the effect of added cellulose on the properties of the resulting bioplastics. Characterization of the prepared bioplastics included FT-IR measurement, surface examination by a scanning electron microscope (SEM) and the determination of mechanical properties by measuring tensile strength, elongation at break and Young’s modulus. It was found that the addition of cellulose from banana peels has a positive effect on the mechanical properties of all prepared agar- or strach-based composites, with the exception of elongation at break in agar films, where there was a slight decrease compared to the reference films.
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Preparation of polysaccharidic binders used in artworks
Švajcrová, Lenka ; Hrdličková Kučková, Štěpánka (advisor) ; Čapek Adamec, Martin (referee)
The main aim of this bachelor thesis was to prepare polysaccharide binders from the chosen kinds of starch (gained from potatoes, corn, wheat, rice and kuzu), Arabic gum and to pigment them. The created samples of the polysaccharide binders were then prepared for further investigation using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and Fourier transform infrared spectroscopy (FT-IR). This work is also focused on the history of the production of starch, its origin, contained substances and growing of the specific plants, from which the starch is obtained from. Furthermore, there is also described the technology process of paintings and other polychromatic arts creation. The technical character of paintings is actually determined by the binders, thus apart from the polysaccharide binders there are also described oil, protein and oil-resin binders. Key words: polysaccharidic binders, starch, Arabic gum, artworks
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Organic-inorganic polymers - synthesis and characterization of hybrid polymers and nanocomposites
Depa, Katarzyna ; Strachota, Adam (advisor) ; Matějka, Libor (referee) ; Sedláček, Jan (referee)
In the first part of this work, silica nanoparticles and alternative or additional filler phases were incorporated into hydrogels based on the temperature-sensitive poly(N- isopropylacrylamide) (PNIPAm). Nano-SiO2-filled porous PNIPAm hydrogels with an enhanced force response (up to 100 g) to temperature stimuli were obtained by increasing several times the pore wall thickness, which was achieved via reducing the solvent (porogen) content during the gels' cryo-synthesis. A similar optimization of the force response was also carried out for analogous gels reinforced by nano-TiO2, in which the reinforcing effect of the filler is weaker. Partial intercalation of amylopectin starch into divinyl-crosslinked bulk as well as porous PNIPAm gels several times improved their extensibility. In case of starch-rich bulk gels, a very fast and extensive one-way deswelling in response to increased temperature was achieved (re-swelling upon cooling is much slower), which is attributed to specific properties of the starch-PNIPAm interface. In doubly-filled bulk PNIPAm/nano-SiO2/starch gels, a very strong synergic reinforcing effect of both fillers is observed, due to specific hydrogen bridging between the three phases. Highly porous cryogels based on PNIPAm/nano- SiO2/starch displayed a highly improved extensibility...
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Stability of controlled drug release systems based on plasticized starch
Zhukouskaya, Hanna ; Štěpánek, Miroslav (advisor) ; Hrubý, Martin (referee)
The thesis is focused on the research of stability of controlled drug release systems based on a blend of plasticized starch/polycaprolactone (TPS/PCL) that served as a carrier. Antibiotic vancomycin was used as a model drug, and its release from TPS/PCL pellets into aqueous environment was followed by UV-spectroscopy and the obtained time dependences were treated by a simple kinetic model. Moreover, the simultaneous release of starch particles to the surrounding liquid phase was studied by static and dynamic light scattering as well as transmission electron microscopy (TEM) in order to obtain information on the stability of biodegradable matrix and on the structure of the products of the pellet decomposition on a nanoscale level. Key words: vancomycin, starch, drug delivery system, polycaprolactone (PCL), particle release, dynamic light scattering (DLS), static light scattering (SLS)
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Tissue-specific knockout of starch synthesis in columella cells of Arabidopsis thaliana and gravitropic response
Bogdan, Michal ; Fendrych, Matyáš (advisor) ; Retzer, Katarzyna (referee)
Since the studies of plant gravitropism by Charles Darwin, the identity of specific sensors of gravity in plants has been uncertain. To this date, statoliths - starch granules in the root tips - are considered to play a key role in gravity sensing. The role of statoliths as organelles that mediate the gravity sensing ability of plant roots is based on research that uses plants which have severely impaired ability to synthesize starch in general or have their cells that contain statoliths removed or damaged. This represents methodical imperfections that give rise to alternative explanations, like disturbed auxin flow due to heavy damage to the root tip or unknown involvement of starch from other parts of the plant in gravity perception. Thanks to advances in the field of CRISPR/Cas9 technology, we are now able to produce tissue-specific mutants that might help with clarification of whether starch granules in the root tip are involved in sensing gravity and if so, how significant is this involvement. This diploma thesis aimed to answer these questions by adapting the tissue-specific CRISPR/Cas9 system and using it for the creation of mutants that are starchless specifically in the columella cells. Using this approach, we generated one tissue non-specific mutant line and three tissue-specific mutant...
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Adhesion of the Hydrogels based on Poly(vinylalcohol)
Přibyl, Jiří ; Trudičová, Monika (referee) ; Kalina, Michal (advisor)
This bachelor thesis was focused on the study of adhesion properties of hydrogels based on polyvinyl alcohol using a rheometer. The used hydrogels were divided into three groups, physical, chemical, and modified with starch, PEG or gelatin. Subsequently, the adhesion properties of the hydrogels were determined using a pull off test measured on a rheometer. From the experimental results, the dependences of the adhesion properties on various parameters such as concentration, molecular weight, freezing / thawing process, amount of glutaraldehyde addition and on the type and ratio of added additive were determined. From the following results, it was found that starch is suitable as an additive for increasing the adhesion properties, and on the other side, PEG can be used to reduce the adhesion properties.
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Solubility control of biodegradable starch-based packaging
Krobot, Štěpán ; Ondreáš, František (referee) ; Lepcio, Petr (advisor)
This bachelor thesis deals with the preparation and testing of biodegradable films based on starch. The aim of the thesis is laboratory preparation of foils based on various types of starch, glycerol, polyvinylalcohol and with the addition of plasticizer – citric acid. Four different types of starch and two types of polyvinyl alcohol were used. In the first series of samples, the ratio of added glycerol to starch content was varied. Samples in the second series were prepared in the same ratios, but with different PVA. A citric acid solution was added into the last series of samples. The second aim of the thesis, after pressing the films, was to determine their solubility and stability in aqueous media depending on the ratio between added glycerol to starch content, the type of polyvinylalcohol used, and the addition of citric acid to the mixture. It has been found that citric acid appears to affect the chemical structure of the films and thus their solubility in aqueous media as well as their mechanical properties. Fourier-transform infrared spectroscopy (FTIR) was used to study the structure of the films and to elucidate its effect on solubility.
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Uplatnění vybraných forem zinku při mimokořenové výživě kukuřice
Pajl, Jaroslav
This thesis deals with the influence of foliar application of selected forms of zinc on the yield and quality of corn grain (Zea mays L.). The issue was dealt with in the form of a small-plot vegetation experiment on the landfill site in Žabčice. Zinc was applied in the 6-8 leaf phase in three forms of ZnO, ZnSO4 and Zn-EDTA at doses of 100, 250 and 500 g.ha-1 in each of them. A zinc-free variant was used as a control. In the course of vegetation (14 days after fertilizer application (20 June 2018)) and after harvesting, an inorganic analysis of the plants (Zn content) was carried out, NDVI and NDRE index were determined during vegetation. After harvest, the yield, starch content and weight of a thousand seeds were determined. Zinc fertilization proved to be (p≤0.05) affected by the zinc content during vegetation, but had no effect on NDVI and NDRE index. Grain yield was positively influenced, although no proven zinc effect was demonstrated. WTS and starch content in the grain were neither demonstrably affected nor significantly different from control. Zinc content in the grain was not significantly affected, but zinc fertilization was positive.
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