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	Protective effect of E. coli strain Nissle 1917 and E. coli strain O83 on the development of inflammation in dextran sulphate sodium-induced colitis in mice Hudcovic, Tomáš ; Štěpánková, Renata ; Hrnčíř, Tomáš ; Kozáková, Hana ; Schwarzer, Martin ; Tlaskalová, Helena The study was focused on the evaluation of probiotic bacteria E. coli strain Nissle 1917 and E. coli strain O83 and their role in the development of intestinal inflammation in DSS model of ulcerative colitis and the route of the application. Intragastrically tubing SCID mice ( with severe combined immunodeficiency) had heavy chages (inflammation) of the colon – part descendens. Intrarectally application had a protective effect on the development of intestinal inflammation – part colon descendens. Proinflammatory cytokines TNF – alpha and IL – 6 were reduce markedly in fragment culture from colon - descendens Detailed record
	Influence of germ-free conditions on the development of sensitization and tolerance to the main compound of brich pollen Bet v 1 in mice Kozáková, Hana ; Repa, A. ; Štěpánková, Renata ; Hrnčíř, Tomáš ; Hudcovic, Tomáš ; Tlaskalová, Helena ; Pollak, A. ; Wiedermann, U. Birch pollen is one of the most frequent airborne allergen of Central and North Europe. The effect of intestinal microflora on sensitization and mucosal tolerance to main component of birch pollen Bet v 1 did not be study yet. Germ-free and conventional mice were administrated intragastrically or intranasally with Bet v 1 following by subcutaneous injections of Bet v 1. Tolerance was established independently on microflora present resulting decreased level of IgE and changed cytokine production Detailed record
	Ontogeny of T- and B- cells in swine Šinkora, Marek Recent molecular and functional studies from our laboratory elucidate key aspects in development of porcine T and B lymphocytes including their ontogeny, behavior, fate, subset discrimination, repertoire and specificity of their cell surface receptors. Our results indicate that rather then immune system of swine is unique its understanding may help to elucidate and elaborate some general assumption and hypothesis based by examination of limited number of species such as humans or rodents Detailed record
	Involvement of NK1.1 receptor in GlcNAc8-conjugate in regulation of antibody formation Huliková, Katarína ; Pospíšil, Miloslav ; Svoboda, Jan ; Kuldová, Markéta ; Fišerová, Anna GlcNAc8-glycoconjugates modulates antigen presentation and B lymphocytes antibody production through NK1.1 receptor triggering Detailed record
	Anti-cancer drugs with immunoprotective/immunomodulatory effect Říhová, Blanka ; Etrych, Tomáš ; Kovář, Marek ; Chytil, Petr ; Hovorka, Ondřej ; Plocová, Daniela ; Mrkvan, Tomáš ; Strohalm, Jiří ; Šírová, Milada ; Šubr, Vladimír ; Kubáčková, K. ; Ulbrich, Karel Cytotoxic drugs based on HPMA polymer show direct cytotoxic and cytostatic effect together with an ability to mobilize the immune system. Two types of linkage of the drug to the polymer are discussed, as well as linkage-related differences in tumour cell accumulation and fate of the cells Detailed record
	Peripheral blood mononuclear cells (PBMC) in infertile women with serum antisperm antibodies produce different spectra of cytokines after in vitro stimulation with sperm cells Ulčová-Gallová, Z. ; Bártová, J. ; Kverka, Miloslav ; Bibková, K. ; Mičanová, Z. ; Tlaskalová, Helena ; Kormunda, S. The aim of this study was to describe the cytokine spectra of PBMC in infertile women and compare them with those in healthy women. The differences in cytokine production may explain the pathogenetic mechanisms that underlie the infertility Detailed record
	Morphological changes in developing cancer of colon in mice induced by combined administration of azoxymethane and sodium dextran sulfate Klimešová, Klára ; Rossmann, Pavel ; Kverka, Miloslav ; Frolová, Lenka ; Tlaskalová, Helena Early stages of carcinoma were documented by light and scanning microscopy, and by immunohistochemical proof of beta-catenin. The study documented the importance of dextran sulfate-induced colitis in the development of mucosal dysplasia and carcinoma Detailed record
	Biologically active components of human colostrum and milk analysed by multiplex proteomic approach Kverka, Miloslav ; Burianová, Jaroslava ; Lodinová-Žádniková, R. ; Kocourková, I. ; Tučková, Ludmila ; Tlaskalová, Helena The lecture discuss use of the antibody array in cytokine spectra analysis in human milk. The role of the newly discovered cytokines for the suckling is discussed Detailed record
	Toll-like receptors -2, -4 and the CD14 molecule in intestinal biopsies of patients with nonspecific intestinal inflammation Frolová, Lenka ; Drastich, P. ; Klimešová, Klára ; Rossmann, Pavel ; Tlaskalová, Helena Expression of TL-2 and TL-4 receptors and of their transmembranes coreceptor CD14 was detected immunohistochemically in biopsies of ileum, colon caecum and rectum of patients suffering from ulcerous colitis or Crohn´s disease. All these three were significantly enhanced in epithelial cells and macrophages of propria in inflammatory bowel disease as compared to the control samples Detailed record
	GlcNAc8-dendrimer as a ligand of NKR-P1A receptor in effector-target cell interaction and G-protein signaling Svoboda, Jan ; Kuldová, Markéta ; Huliková, Katarína ; Miller, R. G. ; Fišerová, Anna GlcNAc8 glycodendrimer is a specific ligand of rat NKR-P1A receptor isoform. In effector cells triggers the Gq/11 protein-mediated signaling pathway, and induces subsequent changes in tumor microenvironment Detailed record

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