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Charakterizace restrikčně-modifikačního systému komensálního kmene
Weiserová, Marie ; Ryu, J.
We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, whose specificity differs from those of known Type IB enzymes, as proved by immunological cross-reactivity and complementation assay. Using a plasmid transformation method and RM search computer program, we have identified the DNA recognition sequence of EcoA0ORF42P as GGA(8N)ATGC
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Kontinuální analýzy DNA translokace enzymem Typu I EcoR124I
Šišáková, Eva ; Seidel, R. ; Szczelkun, M. ; Weiserová, Marie
Using combination of bulk solution and single-molecule assays we have investigated the influence of several mutations in the PD-(D/E)-X-K catalytic motif of HsdR on the DNA translocation and ATPase properties of EcoR124I, the restriction-modification enzyme Type I. The gained results have given us the insight into the possible interdomain interactions between helicase and nuclease domains of the HsdR subunit and the effect of their interactions on DNA translocation
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Spatial and temporal dynamics of enzyme activity distribution in soils with respect to soil fungi
Baldrian, Petr ; Šnajdr, Jaroslav ; Valášková, Vendula ; Cajthaml, Tomáš ; Merhautová, Věra
Activity of laccase, Mn-peroxidase, endo-1,4-β-glucanase, endo-1,4-β-xylanase, cellobiohydrolase, β-glucosidase and β-xylosidase in forest soil (with dominant Quercus sp.) exhibited high but inconsistent variation. Enzyme activity decreased with soil depth as well as fungal biomass and the fungal/bacterial biomass ratio
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