Národní úložiště šedé literatury Nalezeno 24 záznamů.  začátekpředchozí21 - 24  přejít na záznam: Hledání trvalo 0.01 vteřin. 
Microfluidic device for cell counting and electrical lysis
Václavek, Tomáš ; Křenková, Jana ; Foret, František
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
Dvořák, Miloš ; Miková, B. ; Šlampová, Andrea ; Kubáň, Pavel
Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Synthetic approaches for new labeling tags facilitating glycan analysis
Čmelík, Richard ; Křenková, Jana ; Foret, František
This study is focused on the synthesis of labels based on the pyrene core bearing several ionizable quaternary amines and the efficient carbonyl-reactive group, such as primary amine or hydrazine.
Speciation analysis of As and Hg in biological material
Petry-Podgórska, Inga ; Matoušek, Tomáš ; Migašová, M. ; Zemanová, Veronika ; Pavlík, Milan ; Pavlíková, D. ; Kratzer, Jan
This work was focused on an analysis of arsenic in selected plants and mercury in hair. A combination of HPLC and ICP-MS was used for analysis of biological tissues extracts. The\nspeciation analysis of low molecular arsenic and mercury species like inorganic iAs3+ and iAs5+, methylarsenic and dimethylarsenic forms was carried out with hydride generation (HG)\ntechnique, arsenic peptides and inorganic mercury (iHg2+) and its methylated form (MHg+) - with reverse phase (RP) chromatography and detected by ICP-MS.

Národní úložiště šedé literatury : Nalezeno 24 záznamů.   začátekpředchozí21 - 24  přejít na záznam:
Chcete být upozorněni, pokud se objeví nové záznamy odpovídající tomuto dotazu?
Přihlásit se k odběru RSS.