National Repository of Grey Literature 35 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Plant virus-based biotechnology
Vaculík, Petr
The latest model of tertiary structure of capsid protein of potato virus X (PVX CP) was used as a template to design new insertion sites suitable for the preparation of PVX-based antigen presentation system. Based on this model, seven insertion sites (A-G) located in putative surface loops were tested. As an antigen inserted into these sites was used 17 amino acids long epitope derived from human papillomavirus type 16 E7 oncoprotein (E7 epitope) fused with either 6xHis tag or StrepII tag in both possible orientations (6xHis-E7 and E7-6xHis, StrepII-E7 and E7-StrepII). Prior to plant expression, modified PVX CPs were expressed in Escherichia coli MC1061. The results showed that only PVX CP carrying StrepII-E7 or E7-StrepII in the insertion site A formed virus particles. The results from transient expression experiments with modified PVX CPs in Nicotiana benthamiana showed that only the insertion site A (located between 24th and 25th amino acid in the PVX CP) could tolerate all tested inserts. Importantly, viral particles were detected only in the presence of StrepII tag and their stability was affected by the insert orientation (StrepII-E7 vs. E7-StrepII) as only the viral particles presenting E7-StrepII could be purified. Besides the preparation of PVX-based antigen presentation system, an...
Telemetry and Dispatch Control of Low Voltage Grid
Gála, Michal ; Vaculík, Petr (referee) ; Toman, Petr (advisor)
The contents of this thesis are the introduction to mesh grids, the method of dispatch control of these grids and the description of technology used in mesh grids distribution nodes in the city of Brno. This thesis also describes low voltage switchgears used in these types of grids. The development of dispatch control in ECD company is also mentioned. The development describes the grid dispatch control methods prior to implementing the OMS system and the changes which followed after the implementation. The process improvements of the new system resulting from this thesis can be found in this thesis as well. The improvements are incorporated in the OMS system and are used for more efficient dispatch control of the low voltage mesh grids. There is a more detailed analysis of the mesh grid Brno – Bohunice in the practical part of this thesis. The practical part contains analyses of mesh grids stabilized conditions measurements and analyses of mesh grids fault conditions measurements. The analyses assess the power load in transformers, minimal and maximal phase current and maximal power load at the time they were observed. The analysis of the measured data is accompanied by the assessment of differences in current phase measurements and differences in voltage phase and combined measurements. The result, based on the analysis and collection of the data, is a proposition of adding switchgears for the support of the dispatch control of the mesh grid Brno – Bohunice. In the summary of the thesis there is a comparison of the result of the factual and theoretical analysis. An experimental model of the mesh grid Brno – Bohunice was created in PS CAD software as a part of the theoretical analysis.
Plant virus-based biotechnology
Vaculík, Petr ; Čeřovská, Noemi (advisor) ; Ryšánek, Pavel (referee) ; Petrzik, Karel (referee)
The latest model of tertiary structure of capsid protein of potato virus X (PVX CP) was used as a template to design new insertion sites suitable for the preparation of PVX-based antigen presentation system. Based on this model, seven insertion sites (A-G) located in putative surface loops were tested. As an antigen inserted into these sites was used 17 amino acids long epitope derived from human papillomavirus type 16 E7 oncoprotein (E7 epitope) fused with either 6xHis tag or StrepII tag in both possible orientations (6xHis-E7 and E7-6xHis, StrepII-E7 and E7-StrepII). Prior to plant expression, modified PVX CPs were expressed in Escherichia coli MC1061. The results showed that only PVX CP carrying StrepII-E7 or E7-StrepII in the insertion site A formed virus particles. The results from transient expression experiments with modified PVX CPs in Nicotiana benthamiana showed that only the insertion site A (located between 24th and 25th amino acid in the PVX CP) could tolerate all tested inserts. Importantly, viral particles were detected only in the presence of StrepII tag and their stability was affected by the insert orientation (StrepII-E7 vs. E7-StrepII) as only the viral particles presenting E7-StrepII could be purified. Besides the preparation of PVX-based antigen presentation system, an...
Plant virus-based biotechnology
Vaculík, Petr
The latest model of tertiary structure of capsid protein of potato virus X (PVX CP) was used as a template to design new insertion sites suitable for the preparation of PVX-based antigen presentation system. Based on this model, seven insertion sites (A-G) located in putative surface loops were tested. As an antigen inserted into these sites was used 17 amino acids long epitope derived from human papillomavirus type 16 E7 oncoprotein (E7 epitope) fused with either 6xHis tag or StrepII tag in both possible orientations (6xHis-E7 and E7-6xHis, StrepII-E7 and E7-StrepII). Prior to plant expression, modified PVX CPs were expressed in Escherichia coli MC1061. The results showed that only PVX CP carrying StrepII-E7 or E7-StrepII in the insertion site A formed virus particles. The results from transient expression experiments with modified PVX CPs in Nicotiana benthamiana showed that only the insertion site A (located between 24th and 25th amino acid in the PVX CP) could tolerate all tested inserts. Importantly, viral particles were detected only in the presence of StrepII tag and their stability was affected by the insert orientation (StrepII-E7 vs. E7-StrepII) as only the viral particles presenting E7-StrepII could be purified. Besides the preparation of PVX-based antigen presentation system, an...
Automatic Voltage Control of Distributed Generation in the E.ON Medium Voltage Grid
Skoupý, Martin ; Vaculík, Petr (referee) ; Toman, Petr (advisor)
Content of this master`s thesis is theoretical introduction of the power factor, reactive power and voltage control of decentralised sources of the high voltage in the E.ON distribution network. Furthermore, the thesis deals with possibilities of regulating the power factor and reactive power, carried on above-mentioned resources, in other to stabilize the voltage and overflow control of the reactive power to higher voltage level. The practical part describes how the automatic power factor and voltage control had been put into action by central management control within the headquaters dispatching systém of the company. Following chapter acquaints a reader with information of how the automatic power factor and voltage control had been tested and how it is utilized in practice. In the conclusion the work summarizes results and effects of the power factor regulation and voltage control to stabilize the voltage and the overflow of the reactive power in the E.ON network.
Molecular-biological characterization of Potato virus M and Potato leafroll virus
Vaculík, Petr ; Čeřovská, Noemi (advisor) ; Fousek, Jan (referee)
The main aims of diploma thesis were: 1) The sequence analysis of the Czech isolate of Potato virus M (PVM) VIRUBRA 4/009 and phylogenetic analysis of PVM coat proteins sequences 2) The bacterial expression of recombinant triple gene block protein 1 (TGB1) of PVM derived from the Czech isolate VIRUBRA 4/007 3) The construction of expression cassette of Potato leafroll virus (PLRV) coat protein and its transformation into A. tumefaciens for transgenic PLRV resistant plant formation In theoretical part of the thesis the taxonomic classification, morphology, genomic structure and virus transmission are discussed. Furthermore, the main rules concerning the bacterial expression of recombinant proteins and construction of transgenic plants using A. tumefaciens are described. Methodical part is devoted to description of generally used molecular biological and immunochemical methods. The following results were obtained in the thesis: The complete nucleotide sequences of open reading frames coding for three movement proteins (Triple gene block -TGB), coat protein and NA-binding protein of PVM isolate VIRUBRA 4/009; phylogenetic analysis was performed; the TGB1 protein was expressed in bacterial cells and will be used for polyclonal antibodies raising. Finally, the expression cassette containing the PLRV...
Proposal to upgrade the wastewater treatment technology in the selected Wastewater Treatment Plant
Líkař, Vladislav ; Vaculík, Petr (advisor) ; Malaťák, Jan (referee)
This thesis deals with the issue of waste water treatments from small sources of pollution. At the beginnig, there are characterized wastewater and important indicators for measuring the concentration of pollution, then this thesis describes the different technologies and technological equipment used in the treatment of wastewater from small sources. In the practical part of the work is designed to upgrade the technological part for processing waste water for area businesses METRO NITRA. The first part describes curent conditions of selected wastewater treatment plant. Next part describes in detail the proposed technological part and evaluation samplings. Later in this chapter is described the economic value and the expected schedule of work.The aim is to design WWTP upgrade with the technical and the economic assessment.
Integration assitive systems with IH&S
Čáslavková, Andrea ; Votruba, Zdeněk (advisor) ; Vaculík, Petr (referee)
The thesis is focused on the elderly population - seniors and their safe living in their own environment. The main target is to improve their quality of living, independence and self-sufficiency through assistive and security services. We will acquaint with the various organizations that deal with this issue. As an example we will show you a senior´s flat that is equipped with points and elements falling into these technologies and overall assessment.

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2 Vaculík, Pavel
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