National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Bacterial populations in mucosal tissues of the house mouse
Ptáčníková, Aneta ; Stopka, Pavel (advisor) ; Hampl, Vladimír (referee)
Microbiota becomes one of the most important subjects in biological research and numerous studies revealed that microbiota plays a broad spectrum of essential roles in different organisms. This master thesis focuses on the bacterial part of microbiota contained in mucosal tissues of wild house mice (Mus musculus musculus). Male and female samples were collected by nasal and oral cavity lavages, vaginal mucosa lavages and from urine and stool. We aimed to detect quantitative, qualitative and sex-specific differences in bacterial populations between mucosal tissues with particular focus on bacterial cycling in vaginal mucosa during the estrous cycles. Bacterial abundances were estimated by qPCR whilst bacterial diversity was detected by targeted metagenomic sequencing of the hypervariable region of the 16S rRNA gene. Significant differences were detected in bacterial abundances and alpha diversity between particular mucosal tissues. Stool samples contained the highest number of bacteria, while samples from the nasal mucosa and urine contained low amount of bacteria. The highest alpha diversity was discovered in stool samples, the least alpha diversity was found in the urine. Mucosal tissues also varied based on the bacterial composition on the level of particular genera. Detailed analysis of estrous cycles...
Evolutionary apects of dog domestication and hybridization with wolf
Ptáčníková, Aneta ; Hulva, Pavel (advisor) ; Černá Bolfíková, Barbora (referee)
The domestic dog (Canis lupus familiaris) is one of the most widespread domestic animal species over the world. A lot of scientific studies are focused on explaining the process of dog domestication. However, the results of these studies vary in most cases. The conclusion about the origin of dogs is complicated due to hybridization with their ancestor, the gray wolf (Canis lupus), in ancient times and also because of some archaeological discoveries, which are difficult to identify. The origin of various adaptations during domestication process allows dogs to live alongside humans. These adaptations are related to behavior and food digestion process. Hybridization between dogs and wolves in nature is a current issue. It could threaten the genetic integrity of expanding the European wolf population.