Národní úložiště šedé literatury Nalezeno 3 záznamů.  Hledání trvalo 0.01 vteřin. 
PCR identification of selected bacteria in cheeses
Gregušová, Barbora ; Burdychová, Radka (oponent) ; Španová, Alena (vedoucí práce)
This work is focused on identification and species specification of a collection of 44 clostridial strains using molecular-genetic methods. DNA of bacteria isolated from late-blowing defected cheeses was used. The purified DNA was diluted to 10 ng/µl and its ability to be amplified was verified by PCR with universal primers. By genus-specific PCR was proved that DNA of all samples belongs to Clostridium genus. Based on species-specific PCR reactions, it was determined that 7 strains belong to C. butyricum and 12 strains belong to C. tyrobutyricum. 15 strains were positively detected in both species-specific PCRs and therefore identified as mixed cultures. Another 10 strains were not classified into tested species. Strains with the gene encoding the enzyme hydrogenase hydA were searched using PCR. Specific PCR products for this gene were detected in 30 strains of the analysed collection. Especially intense were the amplicons by all strains belonging to C. butyricum species.
PCR identification of selected bacteria in cheeses
Gregušová, Barbora ; Burdychová, Radka (oponent) ; Španová, Alena (vedoucí práce)
This work is focused on identification and species specification of a collection of 44 clostridial strains using molecular-genetic methods. DNA of bacteria isolated from late-blowing defected cheeses was used. The purified DNA was diluted to 10 ng/µl and its ability to be amplified was verified by PCR with universal primers. By genus-specific PCR was proved that DNA of all samples belongs to Clostridium genus. Based on species-specific PCR reactions, it was determined that 7 strains belong to C. butyricum and 12 strains belong to C. tyrobutyricum. 15 strains were positively detected in both species-specific PCRs and therefore identified as mixed cultures. Another 10 strains were not classified into tested species. Strains with the gene encoding the enzyme hydrogenase hydA were searched using PCR. Specific PCR products for this gene were detected in 30 strains of the analysed collection. Especially intense were the amplicons by all strains belonging to C. butyricum species.
Metodika užití jednorázového setu pro detekci měďnatých (Cu2+) iontů v pitných a průmyslových odpadních vodách
Stloukal, R. ; Vopálenská, I. ; Watzková, J. ; Gregušová, B. ; Palková, Z. ; Váchová, Libuše ; Pichová, Iva
Metodika shrnuje aktuální legislativní požadavky a normy související s obsahem měďnatých (Cu2+) iontů v pitných a průmyslových odpadních vodách. V metodice je dále podrobně popsán nově vyvinutý jednorázový detekční set LentiKats, který je založen na detekci měďnatých iontů pomocí Biokatalyzátoru lentikats s imobilizovanými rekombinantními kvasinkami.

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2 Gregušová, Barbora
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