|
|
Analysis of antimicrobial peptides by capillary electrophoresis
Ehala, Sille ; Niederhafner, Petr ; Čeřovský, Václav ; Řezanka, P. ; Sýkora, D. ; Král, V. ; Kašička, Václav
Capillary electrophoresis was used for the purity degree determination of new antimicrobial peptides: octadecapeptide melectin and tetradecapeptide mastoparan PDD-B, and their synthetic analogues. Besides, four semiempirical models correlating electrophoretic mobilities of these peptides with their charge and size (relative molecular mass) were applied to predict their probable structures in solution. Additionally, capillaries coated with gold nanoparticles were employed for the separation of mixtures of antimicrobial peptides.
|
|
| |
|
| |
|
|
Capillary electrophoresis employed for quantitative characterization of peptide interactions with small ions and cyclodextrins
Kašička, Václav ; Ehala, Sille ; Šolínová, Veronika ; Schimperková, Tereza ; Sázelová, Petra ; Koval, Dušan ; Makrlík, E.
Capillary affinity electrophoresis has been applied to investigate quantitatively two types of non-covalent peptide interactions: i) complexes of pseudopeptide valinomycin with ammonium ion and alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol, and ii) complexes of enantiomers of dipeptide beta alanyl-D,L-tyrosine and its derivatives with chiral selector, 2-hydroxypropyl-beta-cyclodextrin, in water.
|
|
|
Investigation of peptide complexes with small cations and cyclodextrins by capillary affinity electrophoresis
Kašička, Václav ; Ehala, Sille ; Šolínová, Veronika ; Schimperková, Tereza ; Sázelová, Petra ; Koval, Dušan
Capillary affinity electrophoresis was applied to quantitative evaluation of the strength of the complexes of i) valinomycin, macrocyclic dodecadepsipeptide ionophore, with small cations (ammonium and alkali metal ions, Li+, Na+, K+, Rb+ and Cs+) in methanol, and ii) enantiomers of dipeptide beta-alanyl-D,L-tyrosine and its derivatives with a chiral selector, 2 hydroxypropyl-beta-cyclodextrin (2-HP-beta-CD), in water.
|
|
|
Characterization of valinomycin binding with alkali metal ions by capillary affinity electrophoresis and density functional theory
Ehala, Sille ; Dybal, Jiří ; Makrlík, E. ; Kašička, Václav
Capillary affinity electrophoresis (CAE) and quantum mechanical density functional theory (DFT) have been applied to the study of non-covalent binding of valinomycin (Val), an antibiotic dodecadepsipeptide ionophore, with alkali metal ions, Li+, Na+, K+, Rb+ and Cs+, in methanol. The binding constants (Kb) of Val-alkali metal ion complexes were evaluated by CAE. The most probable structures of the Val-alkali metal ion complexes were proposed by DFT.
|
|
| |
host ::
RSS.