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The Use of ADS-B Information for Airborne Separation and Collision Avoidance
Jošth Adamová, Eva ; Jonák, Jaroslav (oponent) ; Novák, Andrej (oponent) ; Vosecký, Slavomír (vedoucí práce)
Safety of General Aviation (GA) has always been a concern since lack of harmonized technical standards addressing performance for devices allowing GA aircraft to see and be seen, is major impediment to their widespread use in Europe. The increasing complexity and density of air traffic, when the skies become more crowded with a mix of different airspace users, including unmanned aircraft systems (UAS) trending in the last few years, emphasize the importance of and the need of change. The aim of this doctoral thesis is to elaborate on the possibilities to improve the operational safety of GA operations in uncontrolled airspace anticipating considerable challenges associated with UAS uptake. With the overall ATM framework being adapted to accommodate these novel airspace users, ADS-B technology is being recognized for its significant potential. This thesis explored the possibilities to improve cooperative surveillance in uncontrolled airspace (starting with but not limiting to ADS-B), and through set of experiments evaluated the acceptability, feasibility and reusability of different existing collision avoidance and situation awareness systems, both tailored and not tailored for GA. Part of the research was also the investigation on possible adaptation of the drone dedicated Remain Well Clear concept for GA operational needs. The research activities within the scope of this thesis were undertaken in two phases. Within the first phase, spanning from 2015 to 2019, a series of experiments were conducted. The second phase focused on the exhaustive analysis of systems introduced since the last experiment, culminating in the recent months, highlighting the solutions that with appropriate adjustments hold the potential to be effectively tailored for adoption by GA.
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Airport operations management - application on regional airport
Jošth Adamová, Eva ; Lang, Radek (oponent) ; Šplíchal, Miroslav (vedoucí práce)
The main content of this master´s thesis is Airport Operations Management and it´s application on regional airport Brno – Tuřany. The study analyses common airport operations at the airports and compares it with actual situation at Brno airport. Focusing on comparsion, new upgrading or suggestions for increasing the operations are made. The part of thesis encloses also an approach to contemporary issues in greening the airports and air traffic.
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Silybum marianum in vitro - abiotická elicitace
Adamová, Eva ; Tůmová, Lenka (vedoucí práce) ; Martin, Jan (oponent)
Elicitace je metoda, která může ovlivnit produkci sekundárních metabolitů v rostlinných tkáních. Jednou z možností elicitace je působení elektrického proudu s různým rozsahem hodnot napětí. In vitro kultury Silybum marianum kultivované na médiu dle Murashigeho a Skooga byly vystaveny elektrickému proudu o intenzitě 50 mA a hodnotách napětí 5, 7, 8, 10, 15, 20 a 24V po dobu 10, 30 a 60 minut. Množství jednotlivých složek silymarinového komplexu bylo analyzováno metodou HPLC. Nejvyšší nárůst taxifolinu (0,04 %), silychristinu (0,28 %), silydianinu (0,06 %) v suspenzní kultuře byl zaznamenán po elicitaci elektrickým proudem o napětí 8V po dobu působení 10 min a době odběru 6 hodin. Další zvýšení obsahu jednotlivých složek silymarinového komplexu (taxifolin, silychristin, silydianin) bylo zaznamenáno při působení elektrického proudu o napětí 8V (60 minut, 6 hodin) a 10V (60 minut, 24 hodin). Ostatní testované hodnoty napětí elektrického proudu neovlivnily významně nárůst obsahových látek.
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Silybum marianum in vitro - abiotická elicitace
Adamová, Eva ; Tůmová, Lenka (vedoucí práce) ; Martin, Jan (oponent)
Elicitace je metoda, která může ovlivnit produkci sekundárních metabolitů v rostlinných tkáních. Jednou z možností elicitace je působení elektrického proudu s různým rozsahem hodnot napětí. In vitro kultury Silybum marianum kultivované na médiu dle Murashigeho a Skooga byly vystaveny elektrickému proudu o intenzitě 50 mA a hodnotách napětí 5, 7, 8, 10, 15, 20 a 24V po dobu 10, 30 a 60 minut. Množství jednotlivých složek silymarinového komplexu bylo analyzováno metodou HPLC. Nejvyšší nárůst taxifolinu (0,04 %), silychristinu (0,28 %), silydianinu (0,06 %) v suspenzní kultuře byl zaznamenán po elicitaci elektrickým proudem o napětí 8V po dobu působení 10 min a době odběru 6 hodin. Další zvýšení obsahu jednotlivých složek silymarinového komplexu (taxifolin, silychristin, silydianin) bylo zaznamenáno při působení elektrického proudu o napětí 8V (60 minut, 6 hodin) a 10V (60 minut, 24 hodin). Ostatní testované hodnoty napětí elektrického proudu neovlivnily významně nárůst obsahových látek.
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Airport operations management - application on regional airport
Jošth Adamová, Eva ; Lang, Radek (oponent) ; Šplíchal, Miroslav (vedoucí práce)
The main content of this master´s thesis is Airport Operations Management and it´s application on regional airport Brno – Tuřany. The study analyses common airport operations at the airports and compares it with actual situation at Brno airport. Focusing on comparsion, new upgrading or suggestions for increasing the operations are made. The part of thesis encloses also an approach to contemporary issues in greening the airports and air traffic.
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Sample preparation for single cell analysis
Adamová, Eva ; Basova, E. Y. ; Potáčová, Anna ; Foret, František ; Matalová, Eva ; Klepárník, Karel
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
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Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Matalová, Eva ; Adamová, Eva ; Klepárník, Karel
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
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Senzitive method of Caspase-3 detection in single stem cell
Adamová, Eva ; Klepárník, Karel ; Matalová, Eva
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
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