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Semi-supervised learning in Optical Music Recognition
Mayer, Jiří ; Pecina, Pavel (advisor) ; Straka, Milan (referee)
Optical music recognition (OMR) is a niche subfield of computer vision, where some labeled datasets exist, but there is an order of magnitude more unlabeled data available. Recent advances in the field happened largely thanks to the adoption of deep learning. However, such neural networks are trained using labeled data only. Semi-supervised learning is a set of techniques that aim to incorporate unlabeled data during training to produce more capable models. We have modified a state-of-the-art object detection archi- tecture and designed a semi-supervised training scheme to utilize unlabeled data. These modifications have successfully allowed us to train the architecture in an unsupervised setting, and our semi-supervised experiments indicate improvements to training stability and reduced overfitting. 1
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Inflammatory and Fibrotic Myocardial Involvement
Šedivý, Jakub ; Hromádka, Milan (advisor) ; Mayer, Otto (referee) ; Pařenica, Jiří (referee)
1 Inflammatory and fibrotic myocardial involvement MUDr. Jakub Šedivý, Kardiologické oddělení FN Plzeň Introduction: The aim of my work was to evaluate the comprehensive approach to diagnosis and eventually monitoring the trend in two cardiac diseases of non-ischemic etiology. The first part is focused on evaluation of the inflammatory changes in patients with "infarct-like" myocarditis based on magnetic resonance (MRI) and laboratory tests. We also considered the possibility of restricting the length of physical activity restriction by bicycle ergometry. The second topic was the comprehensive assessment of cardiac risk in patients with systemic sclerosis, including the assessment of possible MR and new laboratory markers of myocardial fibrosis. Materials and methods: In the first part, we included prospectively 30 patients with "infarct- like" myocarditis and with a positive finding of late gadolinium enhancement (LGE) on MRI. Follow up MRI, echocardiography and stress ECG were performed at 1 and 6 month intervals. In the study focused on systemic sclerosis, a total of 33 long-term patients were prospectively enrolled. The study included a comparison of a group of 20 healthy volunteers. Results: In 47% of patients with infarct-like myocarditis, we found a left ventricular dyskinesia, but only 17% of...
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Therapeutic drug monitoring in clinical practice and research
Ďuricová, Jana ; Grundmann, Milan (advisor) ; Mayer, Otto (referee) ; Vítovec, Jiří (referee)
Introduction: The human cytochrome P450 2D6 (CYP2D6) is involved in the oxidative metabolism of about 25 % of all commonly prescribed drugs. It is characterized by high range of interindividual variability due to both environmental and genetic factors. The ability to measure the activity of CYP2D6 enzyme is of high significance. Genotyping alone is not sufficient to accurately predict an individual's actual CYP2D6 activity, phenotyping on the other hand can determine the exact enzymatic activity as it also reflects non-genetic factors. Beta-blocker agent metoprolol undergoes extensive pre-systemic elimination, with enzyme CYP2D6 accounting for about 70 to 80 % of its metabolism. Metoprolol also serves as one of the probe drugs of CYP2D6. The metabolic ratio of metoprolol over its metabolite -hydroxymetoprolol in plasma 3 hours after metoprolol administration is used for the measurement of CYP2D6 enzyme activity. Aims: To compare CYP2D6 metabolic activity after first metoprolol dose and in steady state. Further to investigate the influence of CYP2D6 activity on metoprolol pharmacokinetics and pharmacodynamics in patients on metoprolol therapy. Methods: Thirteen adult hypertensive patients in whom an introduction of beta-blocker metoprolol was indicated were included for comparison of CYP2D6...
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Inflammatory and Fibrotic Myocardial Involvement
Šedivý, Jakub ; Hromádka, Milan (advisor) ; Mayer, Otto (referee) ; Pařenica, Jiří (referee)
1 Inflammatory and fibrotic myocardial involvement MUDr. Jakub Šedivý, Kardiologické oddělení FN Plzeň Introduction: The aim of my work was to evaluate the comprehensive approach to diagnosis and eventually monitoring the trend in two cardiac diseases of non-ischemic etiology. The first part is focused on evaluation of the inflammatory changes in patients with "infarct-like" myocarditis based on magnetic resonance (MRI) and laboratory tests. We also considered the possibility of restricting the length of physical activity restriction by bicycle ergometry. The second topic was the comprehensive assessment of cardiac risk in patients with systemic sclerosis, including the assessment of possible MR and new laboratory markers of myocardial fibrosis. Materials and methods: In the first part, we included prospectively 30 patients with "infarct- like" myocarditis and with a positive finding of late gadolinium enhancement (LGE) on MRI. Follow up MRI, echocardiography and stress ECG were performed at 1 and 6 month intervals. In the study focused on systemic sclerosis, a total of 33 long-term patients were prospectively enrolled. The study included a comparison of a group of 20 healthy volunteers. Results: In 47% of patients with infarct-like myocarditis, we found a left ventricular dyskinesia, but only 17% of...
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Regulation of cell cycle and DNA damage response in mouse oocytes.
Mayer, Alexandra ; Šolc, Petr (advisor) ; Macůrek, Libor (referee) ; Macek, Milan (referee)
A specific feature of mammalian oocytes is a long prophase I arrest, which can be maintained for many years in humans. The oocytes must ensure robust mechanisms, which can keep them in prophase I, but effectively trigger meiotic resumption when required. Consequently, throughout the maturation of an oocyte, non-erroneous chromosome segregation is a prerequisite for the generation of healthy offspring. In this study we aimed to investigate the new roles of Aurora A (AURKA) and polo-like kinase 1 (PLK1) in the regulation of the cell cycle progression. For this purpose, we used transgenic mice that specifically overexpress wild type (WT-) or kinase-dead (KD-) AURKA in oocytes only, and to study PLK1 we treated oocytes with BI2536, a small molecule inhibitor known to specifically inhibit PLK1 in somatic cells. Our data show, that both AURKA and PLK1 are not essential for meiotic resumption, however they participate in this process. Active AURKA regualtes the increase in microtubule organizing centers (MTOC) in prophase I, which is the first visible marker of resumption of meiosis in oocytes. AURKA activation is biphasic, and the initial increase in MTOC is transient, while full AURKA activation needed for the stability of MTOC requires the activity of Cyclin-dependent kinase 1 (CDK1). We show that PLK1...
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Therapeutic drug monitoring in clinical practice and research
Ďuricová, Jana ; Grundmann, Milan (advisor) ; Mayer, Otto (referee) ; Vítovec, Jiří (referee)
Introduction: The human cytochrome P450 2D6 (CYP2D6) is involved in the oxidative metabolism of about 25 % of all commonly prescribed drugs. It is characterized by high range of interindividual variability due to both environmental and genetic factors. The ability to measure the activity of CYP2D6 enzyme is of high significance. Genotyping alone is not sufficient to accurately predict an individual's actual CYP2D6 activity, phenotyping on the other hand can determine the exact enzymatic activity as it also reflects non-genetic factors. Beta-blocker agent metoprolol undergoes extensive pre-systemic elimination, with enzyme CYP2D6 accounting for about 70 to 80 % of its metabolism. Metoprolol also serves as one of the probe drugs of CYP2D6. The metabolic ratio of metoprolol over its metabolite -hydroxymetoprolol in plasma 3 hours after metoprolol administration is used for the measurement of CYP2D6 enzyme activity. Aims: To compare CYP2D6 metabolic activity after first metoprolol dose and in steady state. Further to investigate the influence of CYP2D6 activity on metoprolol pharmacokinetics and pharmacodynamics in patients on metoprolol therapy. Methods: Thirteen adult hypertensive patients in whom an introduction of beta-blocker metoprolol was indicated were included for comparison of CYP2D6...
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Systematic education in an organisation: the system of education in the company Siemens kolejová vozidla s.r.o.
Mayer, Jan ; Beneš, Milan (referee) ; Tureckiová, Michaela (advisor)
The central issue of this diploma thesis is the term "systematic education in an organization" which is first dealt with on theoretical level in the first chapters of the thesis. Particular emphasis is laid on the explanation of the term and a closer approximation of its meaning. The thesis compares various sources of related literature and the views of individual authors. In the subsequent chapters, individual steps of the cycle of systematic education in an organization are described and related technical terms, particularly from the fields of psychology, andragogy and human resources management, are explained. In its second half (from chapter 7 onwards), the thesis deals with the concrete realization of systematic education in Siemens Kolejová vozidla s.r.o. and uses the same conceptual framework as in the theoretical part of the thesis. This way, the reader becomes familiar with the practical realization of systematic education including its individual steps as they are practically implemented in a company. Based on a theoretical framework, an analysis of a system that operates in practice is designed. In the eleventh chapter, the previous chapters are used for identification of strengths and weaknesses of the education system in the organization mentioned above.
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Ševc, P. ; Janovec, J. ; Výrostková, A. ; Svoboda, Milan ; Grabke, H. J. ; Viefhaus, H. ; Mayer, K. H.
The interfacial and microstructural studies after tempering and long-term aging (steels 9Cr1MoVNbB and 10Cr1Mo1WVNbN) and relaxation strength tests (steel TAF650) were performed. Transgranular cleavage was found to be prevalent fracture mechanism of samples impact fractured at about -100°C. Portion of intergranular facets on analyzed fracture surfaces was extremely low. Low GB concentration of phosphorus (below 1 at.%) was detected in all experimental states. No boron was observed in the AES spectra of the investigated steels.
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Creep Resistance and Microstructural Stability of TAF 650 Steel
Svoboda, Milan ; Fejfarová, Ivana ; Kroupa, Aleš ; Sklenička, Václav ; Mayer, K. H.
The quantification of the particles of M23C6 and Laves phase in TAF 650 steel by means of image analysis and thermodynamic calculation of the amount of equilibrium phases were performed. It is shown that the growth rate of Laves phase is substantially higher than that of M23C6 carbide. The total area occupied by Laves phase precipitates represents 52.1 % of all particles area after creep (5888h, 100 MPa, 650 °C). The equilibrium amount of secondary phases calculated by THERMO-Calc is in good agreement with experimental results.
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