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Type II secretion system in the mitochondria of Naegleria gruberi.
Krupičková, Alžběta ; Doležal, Pavel (advisor) ; Hampl, Vladimír (referee)
Several types of the transport (secretion) systems can be found in the membranes of gram-negative bacteria. These systems export proteins into the extracellular milieu of bacteria. One of them, type II secretion system (T2SS), exports the folded proteins through the outer bacterial membrane. The core subunits of T2SS were discovered in the genomes of several protists belonging to the Discoba and Malawimonada groups, including Naegleria gruberi and Malawimonas sp. str. 249. Our experiments suggest that these subunits are localized in the mitochondria of these protists. The discovery of these genes in eukaryotes is surprising. If the T2SS was active in these mitochondria, it would represent an evolutionary intermediate stage of the organelle, which can export and import the proteins. In experimental part of the thesis, we studied how the proteins of N. gruberi and M. sp. str. 249 interact. We demonstrated several interactions, which correspond to the function of bacterial T2SS. To this aim, we employed bacterial and yeast two-hybrid systems. Further, we explored the import of the core subunit of T2SS, pseudopilin, of N. gruberi into the inner mitochondrial membrane. And we also investigated the import of M. sp. str. 249 secretin into the outer mitochondrial membrane.
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Veterinary pharmaceuticals registered in Czech Republic - developmental trends
Krupičková, Anna ; Lamka, Jiří (advisor) ; Vokřál, Ivan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Anna Krupičková Supervisor: prof. RNDr. Jiří Lamka, CSc. Title of diploma thesis: Veterinary pharmaceuticals registered in Czech Republic - developmental trends In the Czech Republic, there is currently no fully complete and functional electronic database of medicinal products intended for animals. Until 2014, veterinary medicinal products were part of the AISLP V database published by SÚKL in Praha. However, this practise ended with release of version 2014.3, following version of AISLP V database no longer contains veterinary products. Certain hope for those interested in veterinary informations has become database named VetInfo, which has been conceived by Cetli s.r.o. since the end for previous form. But the creation of this database is not yet complete, so there are only working and officially not distributed versions for now. A regular part of undergraduate education at our faculty is a subject focused on the issue of veterinary drugs with pharmacological effects; The above-mentioned AISLP V database was, in addition to other resources, repeatedly used for the detailed processing of veterinary issues in the form of diploma theses of the students and the updating of the lessons....
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Type II secretion system in the mitochondria of Naegleria gruberi.
Krupičková, Alžběta ; Doležal, Pavel (advisor) ; Hampl, Vladimír (referee)
Several types of the transport (secretion) systems can be found in the membranes of gram-negative bacteria. These systems export proteins into the extracellular milieu of bacteria. One of them, type II secretion system (T2SS), exports the folded proteins through the outer bacterial membrane. The core subunits of T2SS were discovered in the genomes of several protists belonging to the Discoba and Malawimonada groups, including Naegleria gruberi and Malawimonas sp. str. 249. Our experiments suggest that these subunits are localized in the mitochondria of these protists. The discovery of these genes in eukaryotes is surprising. If the T2SS was active in these mitochondria, it would represent an evolutionary intermediate stage of the organelle, which can export and import the proteins. In experimental part of the thesis, we studied how the proteins of N. gruberi and M. sp. str. 249 interact. We demonstrated several interactions, which correspond to the function of bacterial T2SS. To this aim, we employed bacterial and yeast two-hybrid systems. Further, we explored the import of the core subunit of T2SS, pseudopilin, of N. gruberi into the inner mitochondrial membrane. And we also investigated the import of M. sp. str. 249 secretin into the outer mitochondrial membrane.
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Parasite's life within the host cell.
Krupičková, Alžběta ; Doležal, Pavel (advisor) ; Štáfková, Jitka (referee)
Plasmodium falciparum is one of the most dangerous human intracellular single-celled pathogen. This thesis describes the invasion, the survival and the egress of the pathogen from the host cell (erythrocyte). The main topic of the thesis is the parasite's life within the host cell in a newly created compartment, parasitophorous vacuole. By exploding hundreds of proteins Plasmodium falciparum establishes number of unique modifications inside as well as on the surface of the host cell. The thesis finishes with the chapter on the therapy and possible development of new drugs and the chapter on the parasite evolution. Powered by TCPDF (www.tcpdf.org)
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Legal regulation of the renewable energy resources
Krupičková, Andrea ; Žákovská, Karolina (advisor) ; Damohorský, Milan (referee)
With the adoption of the Act on Promotion of Use of Renewable Energy Resources in 2005, the Czech Republic took a significant step forward to examine the economic field of energy in general in light of environmental and climate protection. With this act, the Czech Republic followed the example of other European Union member states in asserting the goal of reducing greenhouse gas emissions and seeking alternative energy resources in the spirit of the Kyoto Protocol obligations. The goal of this Master's thesis is to present and logically analyze the relevant legal legislation concerning renewable resources passed in the Czech law. The emphasis of this analysis of the Act No. 180/2005 Sb. on the Promotion of the Use of Renewable Energy Resources in the context of energy law, whereby the attention is placed on promotion of energy production, those in the Czech Republic represent two alternative schemes: "feed-in tariff" and "green bonus certificates." With the broad definition of renewable energy resources in the introduction, this thesis comprises a basic overview of this comprehensive legal problem. This thesis consists of five separate chapters, which are closely related.
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DREAMS, DREAMING AND FIGURATIVENESS ACCORDING TO MICHEL FOUCAULT
KRUPIČKOVÁ, Alena
The final paper is composed of two parts. The practical part titled `Dreams and Dreaming{\crq} is a cycle of ten large-scaled monochromatic exposures mutually interconnected by the means of lineation. Exposures were enlarged by the traditional way, on the surface of RC matt sheets. The lineation joints the exposures of the cycle into one theme. Smaller exposures are attached as drafts. The cycle of exposures captures the essence of dreaming in relation to the Michael Foucault{\crq}s work translated into Czech as SEN A OBRAZNOST (since this work was written solely as an introduction to the work of Ludwig Binswanger, Le r\^{e}ve et l{\crq}existence, the French title is unavailable), which is analysed in the theoretical part of the paper.
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Classification of dense shaped refractory products - Part 4: Special products
Kotora, J. ; Korsa, B. ; Krupičková, A. ; Kunc, J. ; Kutzendorfer, J. ; Lvová, H. ; Muller, P. ; Novotný, M. ; Stoklasa, Jaroslav ; Ševčík, F. ; Tomšů, F.
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Methods of test for dense shaped refractory products - Part 17: Determination of bulk density of granular materials by the mercury method with vacuum
Kotora, J. ; Korsa, B. ; Krupičková, A. ; Kunc, J. ; Kutzendorfer, J. ; Lvová, H. ; Muller, P. ; Novotný, M. ; Stoklasa, Jaroslav ; Ševčík, F. ; Tomšů, F.
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Insulating refractory products - Part 6: Determination of permanent change in dimension of shaped products on heating (ISO 2477:1987 modified)
Kotora, J. ; Korsa, B. ; Krupičková, A. ; Kunc, J. ; Kutzendorfer, J. ; Lvová, H. ; Muller, P. ; Novotný, M. ; Stoklasa, Jaroslav ; Ševčík, F. ; Tomšů, F.
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Insulating refractory products - Part 2: Classification of products (ISO 2245:1990 modified)
Kotora, J. ; Korsa, B. ; Krupičková, A. ; Kunc, J. ; Kutzendorfer, J. ; Lvová, H. ; Muller, P. ; Novotný, M. ; Stoklasa, Jaroslav ; Ševčík, F. ; Tomšů, F.
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