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Monitoring the condition of the archaeological sites of Prague Castle
Válek, Jan ; Kozlovcev, Petr ; Kotková, Kristýna ; Fialová, Anna ; Svorová Pawełkowicz, Sylwia ; Frankl, Jiří
In order to study microclimatic conditions, monitoring of selected archaeological terrains of Prague Castle was carried out in the period from August 2020 to May 2023. The internal climate of the area under the third courtyard of Prague Castle, also called Great Excavations, was monitored in the given time range. During the monitoring campaign, sensors were also placed in the area of the Small Excavations and the archaeological terrain of St. George's Monastery at various times. In particular, relative humidity, air and materials temperatures, moisture content of building materials, etc. were monitored. Samples of building materials were also taken and analysed, and the presence of biological degradation agents was monitored. The research focused in detail on describing the temperature and relative humidity of the indoor environment in relation to the moisture content of the materials and their potential degradation agents. These are primarily represented here by water-soluble salts. Their hazardousness in terms of damage to historic materials is influenced by variations in relative humidity and evaporation of water from capillary pores in combination with temperature. Research has shown that the most significant problem for sites is flooding or water seepage through the ground, surrounding structures or ceiling.
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Selection of mortars samples for radiocarbon dating
Válek, Jan ; Kozlovcev, Petr ; Fialová, Anna ; Kotková, Kristýna ; Frankeová, Dita ; Skružná, Olga ; Maříková-Kubková, Jana ; Tomanová, Pavla ; Herichová, Iva ; Světlík, Ivo ; Pachnerová Brabcová, Kateřina ; Šimek, Pavel
Radiocarbon dating of organic residues may not always correctly determine the age of analysed historic mortars. Therefore, it seems advantageous to use of carbon dating of the carbon contained in the structure of the lime mortar, which will provide representative data of the age of the investigated structure. The methodology presents basic instructions and rules for sample selection, processing, and evaluation. The aim of this methodology is the identification of cases where the radiocarbon dating of a binder is appropriate and to define the reasons why some binder systems fail to provide relevant results.
Plný tet:  PDF; PDF
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Determination of reference parameters for evaluating the strengthening of degraded sandstone masonry of the Cathedral of St. Vit at Prague Castle
Válek, Jan ; Kozlovcev, Petr ; Fialová, Anna ; Slížková, Zuzana ; Kotková, Kristýna ; Svoboda, Milan ; Koudelková, Veronika
Sandstone materials exposed to weather conditions degrade naturally, consequently consolidation of these materials is often required. The aim of this research was to evaluate the impact of four commercials organosilicate consolidants on degraded sandstones, which were used during the neogothic rebuilding of st. Vithus Cathedral at the turn of the 19th to 20th century. The depth of penetration of consolidants into the sandstone porous system and the impact of consolidants on the physical properties of sandstones, namely on the hydrofobicity, water absorption and evaporation, were evaluated primarily. According to the test results, all four consolidants should be suitable for consolidation of sandstones mentioned above even though the results varied slightly between consolidants. For final chose of appropriate consolidant, it is necessary to consider the properties of the treated sandstoned and the technological conditions of the application given by the manufacturer.
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Macrophages and nitric oxide in leishmania - sandfly - host interactions
Kratochvílová, Tereza ; Kolářová, Iva (advisor) ; Fialová, Anna (referee)
Leishmania reside fagolysosome of macrophages immediately after their entry to host where they multiply and consequently infect other macrophages or eventually other cells. A synthesis of a reactive reactant of oxygen and nitrogen is one of the mechanisms that some mammal cells are equipped with and that also contributes to eradication of leishmania. Nitric oxide rising during a metabolic change of L-arginine under the catalysis of NO synthase is of a large importance. Beyond cytotoxic function, nitric oxide is involved in signalling pathways for a neurotransmission (nNOS) and vasorelaxation (eNOS). Not all types of macrophages have ability to produce NO (iNOS). It is a heterogeneous group differing in immunological function and also in physiology. A group of classical activated macrophages represents an effective APC capable of efficient killing of intracellular pathogens. In addition to NO, they also secrete an inflammatory cytokines, which evolve an immune reaction towards to Th1. Contrary to this, a group of alternative activated macrophages is not capable of any efficient antigen presentation and nitric oxide production but produces L-ornithine, which is a precursor of polyamines, which leishmania utilizes for its own intracellular growth. For the mouse model, status of resistance and/or...
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Immunity in hosts repeatedly exposed to sand fies and the effect on pathogen transmission
Pohanková, Lucia ; Kolářová, Iva (advisor) ; Fialová, Anna (referee)
6 Abstrakt During the feeding of infected sand flies are inoculate into the host also sand fly saliva, which can strongly modulate the response of the immune system. If the host is naive, the course of infection is usually worse. In cutaneous leishmaniasis, the lesions developed early, are more destructive and persist longer. The hosts living in endemic areas of leishamniasis and their vector hosts are often exposed to feeding uninfected sand flies. For host are the saliva antigenic and induces specific cellular and antibody responses. This responses induce the protection against leishmania infection and differ for different hosts, attempts were made most frequently in murine and canine models. In humans, as hosts is difficult to monitor developments leishmania infection after previous exposure, because in humans mainly monitors the levels of antibodies, by which we can determine the degree of sand fly bites and the risk of transmission of leishmaniasis. Keywords: Lutzomyia, Phlebotomus, DTH, antibody, IgG,
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Macrophages in leishmania - sand fly - host interaction
Kratochvílová, Tereza ; Kolářová, Iva (advisor) ; Fialová, Anna (referee)
Sand flies (order Diptera) are vectors of Leishmania parasites (Trypanosomatida), which are inoculated into the host skin together with the vector saliva. Sand fly saliva plays the important role in the Leishmania transmission; in naive host it supresses the host immune response assisting Leishmania to establish the infection, while in repeatedly bitten host it elicits a protective immune response. The submitted thesis focuses on the effect of sand fly saliva on macrophages, the key cells in the infection control. In the first part of the thesis we established a laboratory model L. major - P. papatasi - Balb/c to describe the protective effect of saliva immunization on Leishmania infection development. Immunized mice were protected against Leishmania infection which was reflected in the ear lesion size, parasite load in the ear dermis and draining lymph nodes but also in cytokine production. On the contrary, produced lower amount of nitric oxide, while arginase activity was comparable with nonimmunized group. The IgG antibodies against saliva served as a marker of exposure to sandflies while IgG antibodies against Leishmania antigens served as a marker of infection severity. The experiments were aimed on the possibility of cross-protectivity in Balb/c mice against L. major between closely related...
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Monitoring of immune parameters during anti-tumor immunotherapy
Bílková, Pavla ; Palich Fučíková, Jitka (advisor) ; Fialová, Anna (referee)
Dendritic cells are the most effective antigen presenting cells in humans, they stimulate naive T lymphocytes and thus initiate specific immune response. The discovery of dendritic cells and understanding of their functions contributed to the idea of usingdendritic cells for the treatment of cancer. Anti tumor immunotherapy is a therapeutic strategy that aims to induce and maintain immune responses against tumor cells. Currently, immunotherapy based on dendritic cells has strong position among other anti cancer therapies and seems to be a promising therapeutic option for patients with tumors. In this work, I evaluated the effectiveness of treatment in patients with prostate cancer treated with immunotherapy based on dendritic cells. I focused on the detection of antigen specific T lymphocytes in peripheral blood against tumor antigens, PSA, NY ESO 1, MAGE A1 and MAGE A3. Using a 3 day standard protocol for the detection of antigen specific T cells using intracellular cytokine staining we were able to detect only a small percentage of this minor population. Only after extension of the protocol, we increased the sensitivity setting and we detected a significantly increased frequency of antigen specific T lymphocytes in the peripheral blood after one year DC vaccines application.
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