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In situ analýzy zánětlivých kolitid Muc2-/- myší.
Drábková, Martina ; Vejsová, Marcela (advisor) ; Jílek, Petr (referee)
Author: Martina Drábková Title: In situ analyses of inflammatory colitis in Muc2-/- mice Diploma thesis Charles University in Prague, Faculty of pharmacy in Hradec Králové Pharmacy Background: Muc2 mucin is the main structural component of the intestinal mucus which forms important protective barrier avoiding the direct contact between the commensal bacteria and the epithelium. In order to understand the role of Muc2 mucin a Muc2 deficient mice strain has been developed. Initial observation showed that these mice already in few weeks after the birth develop spontaneous colitis and later on colon cancer. To better understanding what is going on in the colon and small intestine of Muc2-/- mice, in this thesis we focused on in situ analyses of distribution of different immune cells in these tissues. Methods: In our experiments three Muc2-/- , two Muc2+/- and three wild type mice on the C57BL/6 background were used. For the detection of distribution of different immune cells within lamina propria of small intestine and colon the immunohistochemistry analyses on sections from these tissues were performed. Results: In the lamina propria of distal colon of Muc2-/- mice we found an increase of neutrophils followed by increase of macrophages, CD4+ T cells and B cells/plasma cells compared to control and...
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Application of 3D FESA method in evaluation of the maxillary growth in patients with defects in the orofacial region
Drábková, Martina ; Velemínská, Jana (advisor) ; Sedlak, Petr (referee)
Thesis deals with the study of the longitudinal growth changes of the palate including alveolar pocesses and its variability within the analysed group of 14 patients with UCLP. 28 dental plaster casts obtained from each patient in the two examinations (always before and after the cleft lip surgery), were used for the evaluation process. The first dental plaster cast was taken from patient with an average age of 6 months, while the average age for second continuous casting was 4,5 years. Dental casts were scanned using a 3D laser scanner and then analysed, using methods of geometric morphometrics. The main aim of this study was to evaluate the possibility of FESA application for the analysis of palates with different shapes consisting of two separate maxillary segments. Individual and complete evaluation of the growth changes of palate alveolar processes and palate in patients with UCLP showed that during the monitored period, mutual approximation while narrowing the width cleft occurs, mainly in the anterior part of both maxillary segments. Growth occurs primarily in the posterior parts of both maxillary segments, the most significant changes in the size and shape then correspond to places where new dentition arise. Complementary assessment of size and shape variability of alveolar processes and palate in...
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In situ analýzy zánětlivých kolitid Muc2-/- myší.
Drábková, Martina ; Vejsová, Marcela (advisor) ; Jílek, Petr (referee)
Author: Martina Drábková Title: In situ analyses of inflammatory colitis in Muc2-/- mice Diploma thesis Charles University in Prague, Faculty of pharmacy in Hradec Králové Pharmacy Background: Muc2 mucin is the main structural component of the intestinal mucus which forms important protective barrier avoiding the direct contact between the commensal bacteria and the epithelium. In order to understand the role of Muc2 mucin a Muc2 deficient mice strain has been developed. Initial observation showed that these mice already in few weeks after the birth develop spontaneous colitis and later on colon cancer. To better understanding what is going on in the colon and small intestine of Muc2-/- mice, in this thesis we focused on in situ analyses of distribution of different immune cells in these tissues. Methods: In our experiments three Muc2-/- , two Muc2+/- and three wild type mice on the C57BL/6 background were used. For the detection of distribution of different immune cells within lamina propria of small intestine and colon the immunohistochemistry analyses on sections from these tissues were performed. Results: In the lamina propria of distal colon of Muc2-/- mice we found an increase of neutrophils followed by increase of macrophages, CD4+ T cells and B cells/plasma cells compared to control and...
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Analysis of the Škoda Auto marketing strategy on the Chinese market
Drábková, Martina ; Chylíková, Hana (advisor) ; Novák, Miloš (referee)
The bachelor thesis with the topic "Analysis of the Škoda marketing strategy on the Chinese market" focuses on analyzing the four marketing instruments, which form the current marketing strategy of the Škoda brand on the Chinese market. The aim is to identify the current Škoda position in the competitive environment of the world's biggest automobile market and define the importance and contribution of the Chinese market to achieving Škoda's global goals.
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