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The Use of ADS-B Information for Airborne Separation and Collision Avoidance
Jošth Adamová, Eva ; Jonák, Jaroslav (referee) ; Novák, Andrej (referee) ; Vosecký, Slavomír (advisor)
Otázka bezpečnosti všeobecného letectva bola vždy problematická. Nedostatok harmonizovaných technických noriem týkajúcich sa výkonu zariadení umožňujúcich lietadlám všeobecného letectva vidieť a byť videný, sa stal hlavnou prekážkou k ich rozšírenému používaniu v Európe. Rastúca komplexita a hustota leteckej dopravy, a skutočnosť, že sa vzdušný priestor s príchodom nových užívateľov (napr. systémov bezpilotných lietadiel, ktoré zaznamenávajú v posledných rokoch rastúci trend) stále viac naplňuje, zdôrazňujú dôležitosť a potrebu zmeny. Cieľom tejto dizertačnej práce bolo rozpracovať možnosti zlepšenia prevádzkovej bezpečnosti všeobecného letectva v neriadenom vzdušnom priestore, s prihliadnutím na značné výzvy spojené so zavádzaním bezpilotných lietadiel do vzdušného priestoru. S celkovým rámcom ATM prispôsobujúcim sa týmto novým užívateľom vzdušného priestoru, sa ADS-B technológia so svojim potenciálom stáva významným činiteľom. Táto práca skúmala možnosti zlepšenia kooperatívnej “surveillance” v neriadenom vzdušnom priestore (začínajúc od, ale neobmedzujúc sa na ADS-B) a prostredníctvom súboru experimentov hodnotila prijateľnosť, uskutočniteľnosť, a opätovnú použiteľnosť rôznych existujúcich antikolíznych systémov a “situational awareness” systémov, či už šitých na mieru pre všeobecné letectvo alebo nie. Súčasťou výskumu bolo aj skúmanie možného prispôsobenia konceptu “Remain Well Clear” vyvíjaného pre drony, prevádzkovým potrebám všeobecného letectva. Výskumné aktivity v rámci tejto dizertačnej práce prebiehali v dvoch fázach. V rámci prvej fázy, ktorá trvala od roku 2015 do roku 2019, sa uskutočnila séria experimentov. Druhá fáza sa zamerala na prehľadnú analýzu systémov zavedených od posledného experimentu, ktorá vyvrcholila v posledných mesiacoch. Práca zdôrazňuje riešenia, ktoré s vhodnými úpravami majú potenciál byť efektívne prispôsobené na používanie vo všeobecnom letectve.
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Airport operations management - application on regional airport
Jošth Adamová, Eva ; Lang, Radek (referee) ; Šplíchal, Miroslav (advisor)
The main content of this master´s thesis is Airport Operations Management and it´s application on regional airport Brno – Tuřany. The study analyses common airport operations at the airports and compares it with actual situation at Brno airport. Focusing on comparsion, new upgrading or suggestions for increasing the operations are made. The part of thesis encloses also an approach to contemporary issues in greening the airports and air traffic.
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Silybum marianum in vitro-abiotic elicitation.
Adamová, Eva ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Elicitation is the method to increase the production of secondary metabolites in plant tissue. Elicitation by electric field is one of the method of abiotic elicitation to stimulate plants to produce higher amount of secondary metabolites. In vitro cultures of Silybum marianum were cultivated on Murashige-Skoog medium. Suspension cultures were exposed to electric field (50 mA, voltage 5, 7, 8, 10, 15, 20, 24 V) during 10, 30 and 60 minutes. The content of silymarin complex components was measured by (HPLC). The maximal content of taxifolin (0.04 %), silychristin (0.28 %), silydianin (0.06 %) in suspension culture was detected after elicitation by electric field 8V (10 minutes, 6 hours). The maximal content of taxifolin, silychristin, silydianin in suspension culture was detected after elicitation by electric field 8V (60 minutes, 6 hours) and 10V (60 minutes, 24 hours). The significant increase of silymarin complex components were not detectable after the other values of voltage .
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Silybum marianum in vitro-abiotic elicitation.
Adamová, Eva ; Tůmová, Lenka (advisor) ; Martin, Jan (referee)
Elicitation is the method to increase the production of secondary metabolites in plant tissue. Elicitation by electric field is one of the method of abiotic elicitation to stimulate plants to produce higher amount of secondary metabolites. In vitro cultures of Silybum marianum were cultivated on Murashige-Skoog medium. Suspension cultures were exposed to electric field (50 mA, voltage 5, 7, 8, 10, 15, 20, 24 V) during 10, 30 and 60 minutes. The content of silymarin complex components was measured by (HPLC). The maximal content of taxifolin (0.04 %), silychristin (0.28 %), silydianin (0.06 %) in suspension culture was detected after elicitation by electric field 8V (10 minutes, 6 hours). The maximal content of taxifolin, silychristin, silydianin in suspension culture was detected after elicitation by electric field 8V (60 minutes, 6 hours) and 10V (60 minutes, 24 hours). The significant increase of silymarin complex components were not detectable after the other values of voltage .
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Airport operations management - application on regional airport
Jošth Adamová, Eva ; Lang, Radek (referee) ; Šplíchal, Miroslav (advisor)
The main content of this master´s thesis is Airport Operations Management and it´s application on regional airport Brno – Tuřany. The study analyses common airport operations at the airports and compares it with actual situation at Brno airport. Focusing on comparsion, new upgrading or suggestions for increasing the operations are made. The part of thesis encloses also an approach to contemporary issues in greening the airports and air traffic.
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Sample preparation for single cell analysis
Adamová, Eva ; Basova, E. Y. ; Potáčová, Anna ; Foret, František ; Matalová, Eva ; Klepárník, Karel
Development of sensitive, miniaturized and fast methods aims to reliable analyze even small amounts of samples. Micromanipulation systems such as laser capture microdissection enable to collect just single cells from cell suspensions or tissue samples. One option for further analysis is the bioluminescence reaction based on luciferin/luciferase conversion. This approach was used for detection of active caspases, caspase-3/7. Droplets based microfluidic systems provide benefits in biological, pharmaceutical and chemical research.
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Analysis of tissue-bound cells: Novel approaches using laser capture microdissection
Matalová, Eva ; Adamová, Eva ; Klepárník, Karel
Recent biomedical research prefers analysis of homogenous samples obtained from primary cells to cell lines. Moreover, attention is paid to cells of low number but high biological impact, such as signalling centres in embryonic development, stem cells or tumour cells. To localize cells of interest within an organ/tissue, histological sections are widely used. However, the methods of choice for further analysis of such samples are mostly limited to histochemistry, immunohistochemistry and in situ hybridization.
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Senzitive method of Caspase-3 detection in single stem cell
Adamová, Eva ; Klepárník, Karel ; Matalová, Eva
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
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