Original title: Senzitive method of Caspase-3 detection in single stem cell
Authors: Adamová, Eva ; Klepárník, Karel ; Matalová, Eva
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Keywords: caspase-3; miniaturization; single stem cell
Project no.: GAP206/11/2377 (CEP), EE2.3.20.0182
Funding provider: GA ČR, GA MŠk
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0227392

Permalink: http://www.nusl.cz/ntk/nusl-170547


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers