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Presentation pavilion of WANEMI, a.s. paper-mill in Zábřeh
Říhová, Helena ; Svoboda, Jiří (referee) ; Zemánková, Helena (advisor)
The sense of the diploma paper is to make a new entrance - green belt - for the paper factory - WANEMI. There is the exhibition pavillion and the administration building situated in the green belt. The main point of the diploma is the exhibition pavillion. The project is based on the basic principle of ekological architecture like - demounting, using of the waste water and using of the waste warm air. The elementary pavillions´ shape reminds of a paper folder. There are another small exhibitions and constructions in the park. Everything is united by shape and material. The park is the cultural area for sport and education.
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DNA damage induced with sulphur mustard
Říhová, Helena ; Vopršalová, Marie (advisor) ; Melicharová, Ludmila (referee)
In our study, we used the comet assay to assess the sort and range of DNA damage induced by sulfur mustard. Sulfur mustard belongs to chemical warfare agents and is embedded in the blistering agent category. It reacts with a wide range of macromolecules within cells (e.g. DNA, RNA and proteins) and induces for example alkylation of DNA. The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA, ultraviolet (UV)-induced pyrimidine dimers, oxidized bases, and alkylation damage. The aim of the present study was to find out, how can be relaxed loops related to the amount of present strand breaks within DNA molecule. We were also interested in the mechanism of DNA damage caused by sulfur mustard. The cell lines employed were HeLa, UV-20, A549 and AA8 cells. Initially, we assessed the influence between cross-links caused by sulfur mustard and strand breaks caused by styrene oxide. We found sulfur mustard to be highly potent to form cross-links even in low doses and to prevent relaxation of DNA loop caused by strand brakes. Using selected enzymes, we have identified the sort of DNA damage induced by sulfur mustard. First we used endonuclease III, a protein from E. coli which acts both as N-glycosylase and a AP-lyase. It causes strand brakes in apurinic or...
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DNA damage induced with sulphur mustard
Říhová, Helena ; Vopršalová, Marie (advisor) ; Melicharová, Ludmila (referee)
In our study, we used the comet assay to assess the sort and range of DNA damage induced by sulfur mustard. Sulfur mustard belongs to chemical warfare agents and is embedded in the blistering agent category. It reacts with a wide range of macromolecules within cells (e.g. DNA, RNA and proteins) and induces for example alkylation of DNA. The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA, ultraviolet (UV)-induced pyrimidine dimers, oxidized bases, and alkylation damage. The aim of the present study was to find out, how can be relaxed loops related to the amount of present strand breaks within DNA molecule. We were also interested in the mechanism of DNA damage caused by sulfur mustard. The cell lines employed were HeLa, UV-20, A549 and AA8 cells. Initially, we assessed the influence between cross-links caused by sulfur mustard and strand breaks caused by styrene oxide. We found sulfur mustard to be highly potent to form cross-links even in low doses and to prevent relaxation of DNA loop caused by strand brakes. Using selected enzymes, we have identified the sort of DNA damage induced by sulfur mustard. First we used endonuclease III, a protein from E. coli which acts both as N-glycosylase and a AP-lyase. It causes strand brakes in apurinic or...
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Presentation pavilion of WANEMI, a.s. paper-mill in Zábřeh
Říhová, Helena ; Svoboda, Jiří (referee) ; Zemánková, Helena (advisor)
The sense of the diploma paper is to make a new entrance - green belt - for the paper factory - WANEMI. There is the exhibition pavillion and the administration building situated in the green belt. The main point of the diploma is the exhibition pavillion. The project is based on the basic principle of ekological architecture like - demounting, using of the waste water and using of the waste warm air. The elementary pavillions´ shape reminds of a paper folder. There are another small exhibitions and constructions in the park. Everything is united by shape and material. The park is the cultural area for sport and education.
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