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In the gardens
Nejeschleba, Vojtěch ; Říčný, Jan (referee) ; Mléčka, Jan (advisor)
In my thesis I deal with the theme of the garden in relation to housing and in relation to the city. I apply the theme to the design of a new neighbourhood in the Olomouc suburb of Hejčín. In the design, I refer to the local context both by the chosen theme, but also by the scale and compositional arrangement. I am looking for soft boundaries between private and public space. I am exploring the possibilities of shared gardens. I look for living in community and in gardens.
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The role of endothelin receptors type A and B in the model of focal cerebral ischemia in immature rats
Vondráková, Kateřina ; Tsenov, Grygoriy (advisor) ; Říčný, Jan (referee)
Hypoxic-ischemic insult is a most common form of perinatal brain damage that threatens a newborn's life and can leads to permanent neurological sequelae. However, detailed aspects of the cerebral ischemia in the immature brain stay unanswered. We decide to use the model of focal cerebral ischemia induced by intrahippocampal endothelin-1 (ET-1) in 12-days-old rats. The knowledge about consequences of ET-1 induced ischemia and the role of endothelin receptors (ETA and ETB) in ischemia-induced consequences in immature brain are poor at present. Agonists and selective antagonists of the ETA and ETB receptors were used to determine the role of these receptors in the development of ischemia, changes in regional blood flow and tissue oxygenation, local changes of biochemical parameters and acute neuronal death. Our results indicates, that activation of the ETA receptors causes a strong decrease of the blood flow, induced related hypoxia and subsequent neuronal degeneration, whereas activation of ETB receptors has likely modulatory role. Moreover, ischemia causes increase of excitatory amino acids concentration, whereas inhibitory amino acid, except taurine, decreased after ischemia. These facts provides new insights in a case of perinatal ischemia. This thesis demonstrates the wide range of different effects of...
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Tau protein and its variants in the diagnosis of Alzheimer's disease
Krestová, Michala ; Říčný, Jan (advisor) ; Fialová, Lenka (referee) ; Balaštík, Martin (referee)
It is accepted that fibrillar aggregated tau is the best histopathological correlate of the onset and progression of dementia. Tau protein was long regarded as an intracellular protein with several functions inside of cells. New evidence suggests tau secretion into the extracellular space. It is plausible that both intracellular and extracellular forms of tau protein contribute to AD neurodegeneration. The truncated/fragmented forms of tau protein are prone to self- aggregate and form soluble oligomers which are now considered the toxic agents that spread the pathology in AD and other tauopathies. In addition, immunologic abnormalities including defective immune regulation and autoimmunity have been demonstrated in AD patients. Therefore, we have studied the role of various extracellular forms of tau protein and antibodies against them in AD. Firstly, we showed that antibodies isolated from intravenous IgG (IVIG, product Flebogamma) and plasma of older cognitively healthy persons (controls) were reactive with pathological soluble aggregates (oligomers) of tau protein present in the brain of AD patients. On the contrary, isolated antibodies from the plasma of AD patients revealed reactivity with lower molecular weight (LMW, monomeric) tau forms found in brain tissue. Moreover, the antibodies from...
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Targeting cholinergic system in tretment of Alzheimer's disease.
Golianová, Nikoleta ; Jakubík, Jan (advisor) ; Říčný, Jan (referee)
Alzheimer's disease (AD) is a neurodegenerative disorder of CNS and very serious type of dementia. AD affected 46.8 million people worldwide in 2015, and it is estimated that the number of patients will double every 20 years, reaching over 130 million people in 2050 according to Alzheimer's Disease International. There are two forms of the AD: familial (FAD) and sporadic (SAD) form. FAD is an early-onset disease caused by genetic mutations. SAD is more common, a late-onset disease with the age and ε allele of apolipoprotein E as major risk factors. The most crucial symptom is memory disorder, followed by disorientation, confusion, depression and later on, serious psychical and motor-skill problems. These symptoms are the result of a neuronal loss due to formation of β-amyloid oligomers and neurofibrillary tangles in the central nervous system (CNS). As for now, there are neither efficient diagnostic approaches, nor therapeutic ways to stop the degeneration of the brain. There are some drugs available, such as inhibitors of acetylcholinesterase, that have proven to slow down the progression of the AD. Other cholinergic approaches have been developed, but they have shown a lot of side effects, as they are targeting a large scale of receptors. Additional approaches are focusing on clearance of -...
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Tau protein and its variants in the diagnosis of Alzheimer's disease
Krestová, Michala ; Říčný, Jan (advisor) ; Fialová, Lenka (referee) ; Balaštík, Martin (referee)
It is accepted that fibrillar aggregated tau is the best histopathological correlate of the onset and progression of dementia. Tau protein was long regarded as an intracellular protein with several functions inside of cells. New evidence suggests tau secretion into the extracellular space. It is plausible that both intracellular and extracellular forms of tau protein contribute to AD neurodegeneration. The truncated/fragmented forms of tau protein are prone to self- aggregate and form soluble oligomers which are now considered the toxic agents that spread the pathology in AD and other tauopathies. In addition, immunologic abnormalities including defective immune regulation and autoimmunity have been demonstrated in AD patients. Therefore, we have studied the role of various extracellular forms of tau protein and antibodies against them in AD. Firstly, we showed that antibodies isolated from intravenous IgG (IVIG, product Flebogamma) and plasma of older cognitively healthy persons (controls) were reactive with pathological soluble aggregates (oligomers) of tau protein present in the brain of AD patients. On the contrary, isolated antibodies from the plasma of AD patients revealed reactivity with lower molecular weight (LMW, monomeric) tau forms found in brain tissue. Moreover, the antibodies from...
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Chosen Analogues Derived from Substance 7-MEOTA Action on Some Aspects of Cholinergic System
Sedláček, Lukáš ; Říčný, Jan (advisor) ; Hejnová, Lucie (referee)
This thesis deals with effects of some chosen 7-methoxitacrine (7-MEOTA) analogues on enzymatic activity of acetylcholinesterase (AChE). 7-MEOTA is a derivative of tacrine, which had been used for symptomatic treatment of Alzheimer's disease (AD), until drugs with better therapeutic index were developed. 7-MEOTA the same way as tacrine therapeutically acts by inhibition of acetylcholinesterase and a neurotransmitter acetylcholine rise in the organism. It shows similar strength and type of inhibition, but it's less toxic contrary to tacrine. Some of the previously examined analogues of 7-MEOTA were as strong or even stronger AChE inhibitors than 7-MEOTA and so promising future medicaments. However, all the compounds analyzed in this thesis showed weaker enzymatic reaction inhibition and AChE affinity. For each of the examined compounds IC50, Ki and Ki' were calculated and AChE inhibition type was determined. All the 7-MEOTA analogues showed a mixed type of the inhibition. The theoretical part of this thesis deals with manifestations and origins of AD, its genetic factors etc. and tries to show some of the anthropological findings a theories connected with the theme.
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The role of endothelin receptors type A and B in the model of focal cerebral ischemia in immature rats
Vondráková, Kateřina ; Tsenov, Grygoriy (advisor) ; Říčný, Jan (referee)
Hypoxic-ischemic insult is a most common form of perinatal brain damage that threatens a newborn's life and can leads to permanent neurological sequelae. However, detailed aspects of the cerebral ischemia in the immature brain stay unanswered. We decide to use the model of focal cerebral ischemia induced by intrahippocampal endothelin-1 (ET-1) in 12-days-old rats. The knowledge about consequences of ET-1 induced ischemia and the role of endothelin receptors (ETA and ETB) in ischemia-induced consequences in immature brain are poor at present. Agonists and selective antagonists of the ETA and ETB receptors were used to determine the role of these receptors in the development of ischemia, changes in regional blood flow and tissue oxygenation, local changes of biochemical parameters and acute neuronal death. Our results indicates, that activation of the ETA receptors causes a strong decrease of the blood flow, induced related hypoxia and subsequent neuronal degeneration, whereas activation of ETB receptors has likely modulatory role. Moreover, ischemia causes increase of excitatory amino acids concentration, whereas inhibitory amino acid, except taurine, decreased after ischemia. These facts provides new insights in a case of perinatal ischemia. This thesis demonstrates the wide range of different effects of...
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The development of the relationship of the Civil Code and the Labour Code
Říčný, Jan ; Hůrka, Petr (advisor) ; Štefko, Martin (referee)
The aim of this thesis is to create a comprehensive overview of the development of the relationship of the Civil Code and the Labour Code. This thesis consists of ten chapters, which are further divided into subsections. In the introductory chapter describes the aims and methodology of how to create a text. The following chapters deal with the development of the relationship of the Civil Code and the Labour Code in a certain period, whose boundaries are important milestones in the development of both codes. The second chapter deals with the development of the relationship until 1811. Year 1811 was chosen as a milestones because of the adoption Allgemeines bürgerliches Gesetzbuch für die gesammten Deutschen Erbländer der Österreichischen Monarchie (ABGB), ie the General Civil Code, which was a revolution in the law in our country. Other chapters are devoted to the relationship at each stage. As an additional milestones were selected years 1918 - establishment of an independent Czechoslovak state and the emergence of a new legal system, 1950 - the first codification of labor law, 1989 - The Velvet Revolution and the subsequent transformation of the legal system and the year 2006 - adoption of the new Labour Code. Finding the Constitutional Court file. Pl. Ú.S. 83/06 was the most important milestone...
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Chosen Analogues Derived from Substance 7-MEOTA Action on Some Aspects of Cholinergic System
Sedláček, Lukáš ; Říčný, Jan (advisor) ; Hejnová, Lucie (referee)
This thesis deals with effects of some chosen 7-methoxitacrine (7 MEOTA) analogues on enzymatic activity of acetylcholinesterase (AChE). 7-MEOTA is a derivative of tacrine, which had been used for symptomatic treatment of Alzheimer's disease (AD), until drugs with better therapeutic index were developed. 7-MEOTA the same way as tacrine therapeutically acts by inhibition of acetylcholinesterase and a neurotransmitter acetylcholine rise in the organism. It shows similar strength and type of inhibition, but it's less toxic contrary to tacrine. Some of the previously examined analogues of 7-MEOTA were as strong or even stronger AChE inhibitors than 7-MEOTA and so promising future medicaments. However, all the compounds analyzed in this thesis showed weaker enzymatic reaction inhibition and AChE affinity. For each of the examined compounds IC50, Ki and Ki' were calculated and AChE inhibition type was determined. All the 7 MEOTA analogues showed a mixed type of the inhibition. The theoretical part of this thesis deals with manifestations and origins of AD, its genetic factors etc. and tries to show some of the anthropological findings a theories connected with the theme. Powered by TCPDF (www.tcpdf.org)
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Cell signalling and molecular complexes of the TRH receptor
Drastichová, Zdeňka ; Novotný, Jiří (advisor) ; Hodný, Zdeněk (referee) ; Říčný, Jan (referee)
1 Summary The first part of this thesis is preoccupied with the identification of protein alterations in the membrane fraction of HEK293-E2M11 cells after prolonged TRH treatment. The isolated membrane fraction enriched in plasma membranes contained markedly increased the amount of Na,K-ATPase, TRH receptor and G-proteins compared to the postnuclear supernatant. By using 2D electrophoresis and mass spectrometry, the levels of 42 proteins were identified to be altered in samples of PM- enriched fractions from TRH-treated (16 h; 10 μM) cells. Out of these proteins only ezrin and stomatin-like 2 are known to be localized in the plasma membrane. Five proteins (mitofilin, MTHSP75, prohibitin, stomatin like-2, peroxiredoxin III) whose levels were increased after the prolonged TRH treatment represent proteins localized in mitochondria. All of them are important for proper structure and function of mitochondria. The ratio of anti-apoptotic Bcl-2 to pro-apoptotic Bax was markedly higher in cells treated with TRH than in control untreated cells. Hence, it can be concluded that prolonged TRH treatment may significantly affect mitochondrial membrane and function of mitochondria. The second part of this thesis deals with the identification of molecular protein complexes of TRH-R and/or Gq/11 protein. The presumed...
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