|
|
Automatization of method of surface structure measurement by means of reflection mode digital holographic microscope.
Vacula, Daniel ; Šerý, Mojmír (referee) ; Lovicar, Luděk (advisor)
Reflected-light digital holographic microscope developed at IPE FME BUT uses off-axis holography principle and low spatial and temporal coherence illumination. Microscope allows reconstruction of the image amplitude and the image phase, which can be handled in real time. The only limiting factors are imaging speed of the detector and computer performance when processing holograms. Reconstruction of image phase and amplitude allows high-resolution profilometric measurements in the vertical axis direction. This thesis deals with the automatization of profilometric measurement method proposed in [2]. Proposed method uses the combination of the image phase and the image amplitude for the measurement of specimens with surface structure the vertical size of which cause the uncertainty of the image phase by a factor of 2pí. Futher the thesis deals with the construction design of the illumination system of the microscope and its realization together with experimental verification of functionality of proposed method automatization.
|
|
|
SMV-2015-36: „Light Sheet“ microscope combining three wavelengths
Ježek, Jan ; Kaňka, Jan ; Jákl, Petr ; Šerý, Mojmír
The equipment is used for 3D scanning of fluorescence in biological samples. Three lasers with wavelengths suitable for biological samples are used for the excitation of fluorescent dyes. The image is scanned by a highly sensitive CCD camera. Switching between different excitation lasers is controlled by a computer. The observed sample is held in a holder mounted on a four-axis table (3 axes for moving a sample, one axis of rotation around the axis of the sample). The equipment is used to capture images of the sample in different positions of the positioning table.
|
|
|
SMV-2014-04: Micro-stereolithography writer
Šerý, Mojmír
Contractual research is dealing with experimental development of apparatus for creating of microstructures with strongly focused laser beams. The microstructures are written into photoresist material by two-photon process. It is possible to write details in the range of hundreds of nanometers.
|
|
|
SMV-2013-19: Nanolithography system based on two-photon photopolymerization
Jákl, Petr ; Šerý, Mojmír ; Zemánek, Pavel
The reasearch contract deals with development of experimental setup for dynamic shaping of laser beams. Using computer generated holographic phase masks, it is possible to create independent foci of laser beams in the sample space, change their intensity profiles and power of each diffracted beam. By employing fast acousto-optic modulator, the complex procedure allows control of sample irradiation within microsecond resolution. It is possible to precisely select sample region for laser impact using computer-controlled nano-positioning stage.
|
|
|
SMV-2012-07: Optical tweezers
Zemánek, Pavel ; Jákl, Petr ; Šerý, Mojmír
We developed unique system combining holographic optical tweezers with Raman microspectrometer. Part of the contract research was also laboratory desktop consisting of compact optical tweezers and optical scissors combined with light microscope. Delivery included user friendly program enviroment to fully control all features of provided devices.
|
|
|
From the mere pushing to sorting of microparticles and assembly of microrobots by light
Zemánek, Pavel ; Arzola, Alejandro V. ; Brzobohatý, Oto ; Chvátal, Lukáš ; Jákl, Petr ; Kaňka, Jan ; Karásek, Vítězslav ; Šerý, Mojmír ; Šiler, Martin
We demonstrate the recent progress in the field of optical micromanipulation. We start with the classical applications of solar sail propelled by the radiation pressure and approach the recent ones dealing with optical tractor beams, optical binding and sorting of microobjects. The pioneering attempts to assemble a microrobot by light is presented.
|
|
|
Phase photo masks produced by means of electron beam lithography and ion etching for Bragg gratings
Krátký, Stanislav ; Urbánek, Michal ; Kolařík, Vladimír ; Horáček, Miroslav ; Chlumská, Jana ; Matějka, Milan ; Šerý, Mojmír ; Mikel, Břetislav
Fiber Bragg grating is based on the local changes of refractive index in the core of the optical fiber. It has a wide application area, e.g. different types of filters in communications, and it may also be used in sensing of mechanical stresses. One can use different technologies to prepare this type of grating, e.g. the refractive index can be modified directly during production of an optical fiber. Further, the grating may be exposed point by point by a laser beam. The most effective way is an exposure through a phase mask, since a mask may be used for the production of hundreds of grids. This paper discusses different approaches for the preparation of phase masks in terms of impact on the quality of the exposed Bragg grating. The lattice phase mask is defined mainly by two parameters; period and depth.
|
|
|
Specific forms of users interaction with holographic optical tweezers controlled by leap motion
Keša, P. ; Tomori, Z. ; Kaňka, Jan ; Jákl, Petr ; Šerý, Mojmír ; Bernatová, Silvie ; Antalík, M. ; Zemánek, Pavel
Optical tweezers allows contactless three-dimensional manipulation with electrically neutral objects of sizes from tens of nanometers to tens of micrometers. Mouse or joystick are the traditional devices to control the positions of trapping beams in holographic optical tweezers that provide optical manipulation with microobjects. Our approach is based on a modern 3D depth sensors combined with gestures and speech recognition software. The human operator controls the position of one trapping beam by a traditional pointing device like mouse or joystick. However, manipulation with more objects needs control of corresponding number of independent trapping beams generated by a spatial light modulator. We upgraded own NUI software which processes signals from sensors and sends the control commands to holographic Raman tweezers which subsequently controls the positions of trapping microparticles and the acquisition system of Raman spectra.
|
|
|
Raman tweezers for sorting of living cells
Pilát, Zdeněk ; Ježek, Jan ; Kaňka, Jan ; Šerý, Mojmír ; Jákl, Petr ; Zemánek, Pavel
We have developed an instrument for automatized analysis and sorting of living cells of unicellular algae and other micro-objects based on laser tweezers and Raman spectroscopy. The system comprises the Raman tweezers setup, special microfluidic chip, and a specialized software allowing image recognition, spectral analysis, and automated sorting functions. The resulting instrument allows non-destructive analysis of chemical properties of living cells and their automatic separation for further examination or cultivation.
|
|
| |
guest ::
RSS feed.