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The Influence on Production of Secondary Metabolits in Tissue Culture Hypericum perforatum (L.)
Machačková, Jana ; Martin, Jan (advisor) ; Tůmová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Farmacognosy Candidate Mgr. Jana Machačková Consultant PharmDr. Jan Martin, Ph.D. Title of Thesis The influence on production of secondary metabolits in tissue culture Hypericum perforatum (L.) Using of precursors is one of the biotechnological methods, where precursors are added to the culture medium. Callus and suspensions cultures of Hypericum perforatum L. were used. Fenylalanin and cinnamoyl acid in concentrations 20,0 g/l, 2,0 g/l and 0,2 g/l, resp. 25,0 g/l, 2,5 g/l and 0,25 g/l were used as precursors. The effect of precursors on the production of flavonoids was evaluated after 4, 24 and 168 hours. Cultures were cultivated on Murashige and Skoog medium with addition 2 mg/ml growthing hormon BAP. HPLC method for analysis was used. Generally, the highest increase of flavonoids was made by precursor fenylalanin in concentration B in callus and suspensions cultures. The top in production of quercitrin was detected in suspension cultures after application fenylalanin in concentration C after 168 hours cultivation (from 0,16 % to 0,22 %). Hyperosid was the most increased by fenylalanin in concentration C after 4 hours cultivation (from 0,24 % to 0,35 %). Cinnamoyl acid as precursor is not suitable for increase...
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Rights to sign in Czech positive law
Macháčková, Jana ; Pítra, Vladimír (advisor) ; Růžička, Michal (referee)
and keywords The purpose of the thesis is to analyse the legal regulation and the system of registration of rights to signs, which are most commonly used on national as well as international markets, i.e. rights to a trademark, appellation of origin and a geographical indication. The aim of this thesis therefore includs definition of the registration system of the mentioned rights to sign with reference to their specifications, the registration ability conditions (taking into consideration the decision practise of the Industrial Property Office), as well as the different character of protection of their owners. Significant attention is also paid to the system of legal protection of the rights to sign with respect to the fact, that there exists the national, international and Communitary system of protection of these rights. The beginning of the thesis describes the structure of rights to sign, the corresponding specification of concept of intellectual and industrial property rights and it characterises the subject of industrial property rights with respect to the legal regulation, the conception of legal protection of industrial property rights and conditions of grant of protection. The introductory text deals with the general exposition to rights to sign and mainly to historical circumstances of...
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Joint reflection in pre-servise primary school mathematics teacher training
Macháčková, Jana ; Tichá, Marie (advisor) ; Vondrová, Naďa (referee) ; Hošpesová, Alena (referee)
TITLE: Joint reflection in pre-servise primary school mathematics teacher training AUTHOR: Jana Macháčková DEPARTMENT: Department of mathematics and didactics of mathematics SUPERVISOR: Mgr. Marie Tichá, CSc. Abstract: The thesis focuses on the potential of development of pre-service and in- service teachers' self-reflection as one of the ways leading to development of pedagogical content knowledge. The thesis starts with definitions of the background concepts (teacher competences, pedagogical content knowledge, reflection, self- reflection, joint reflection). Also, as the experiments were carried out in the environment of fraction, the introductory part discusses issues related to interpretation and representation of fractions and sources of problems with fractions, their interpretation and representation. The first aim of the research was to monitor the quality of teachers' reflections. The subsequent goal was, on the basis of findings of this monitoring, to look for ways leading to refinement of this reflection. When monitoring and developing reflections, the main method used was the method of joint reflection of a video recording of a lesson. However, other methods were employed simultaneously (observation, group interview). Materials collected in the experiments (students' production, students'...
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Perception and Interpretation of Fractions by Pupils in the 5th Grade of Primary School
Vašíčková, Jana ; Tichá, Marie (advisor) ; Macháčková, Jana (referee)
The essential part of this diploma thesis is a classification of works obtained from an investigation aimed on determination of conceptions of fractions by pupils in the fifth form of the primary school. The experiment consists of four tasks, which were given to children in four different classes. I have classified produced works according to bases of division, which I had established. Mainly concerned classifications are the thematic classifying, classifying in accordance with the "type of children's works" and classifying based on occurred interpretations of fractions. I was trying to find such criterions that would state in evidence of what conceptions and knowledge of fractions do the pupils in the fifth form have. According to gained results I think that I fulfilled this goal. When I was sorting the works of children, I found out that there is a huge amount of large-scaled works that can become basis for another research.
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In vitro cultures of medicinal of plants - VII
Machačková, Jana ; Martin, Jan (referee) ; Tůmová, Lenka (advisor)
In vitro cultures of medicinal of plants - VII Elicitation is one of the methods that can be used to increase production or accumulation of secondary metabolites in explants cultures. There were used callus and suspensions cultures of Silybum marianum (L.) Gaertn. Derivate N-phenylpyrazin-2-carboxamid was used as abiotic elicitor in concentrations c1=1.159x10-3 mol/l, c2=1.159x10-4 mol/l and c3=1.159x10- 5 mol/l. The effect of elicitors on the production (flavonolignans of silymarin complex and flavonoid of taxifolin) was evaluated after 6, 12, 24, 48, 72 and 168 hours. Cultures were cultivated on Murashige and Skoog medium with addition 10 mg/ml α-naphtylacetic acid (α- NAA). HPLC method for analysis was used. The maximal content of flavonolignans was reached after elicitor application in concentration c2 after 168 h in callus culture (0,08%). However the highest content of silydianin was detected after application of elicitor in concentration c1 (0.11 %) in suspensions culture. The maximal content of flavonoid taxifolin was noted in of nutritient medium of suspensions cultures.
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A Floristic Study of Petřín
Macháčková, Jana ; Dostál, Petr (advisor) ; Skýbová, Jana (referee)
The first part of the thesis describes culture-historical and natural features of the gardens at Petřín. The reader learns about the protected area in the upper part of the gardens and crucial influences of human activity in this area in the past and present. The second part deals with the results of a dendrological survey carried out in the Kinský Garden and Nebozízek and floristic research of the nature monument Petřínská skalka in the period between spring 2004 and autumn 2005. The results of the mapping have been recorded in table files that relate to attached documentary maps. In the discussion, I compare the findings of the thesis to the results of inventoried materials and suggest possible causes of the differences obtaining between the two lists. In the didactic part, I suggest a way of using this area for educational purposes. It contains two tracks - one focused on timber species and the second on complex cognition of nature. Worksheets were for both the tracks. The text also includes documentary photographs of some monitored timber species and plants. Powered by TCPDF (www.tcpdf.org)
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On one of the ways to improve pedagogical content knowledge
Macháčková, Jana
I consider that one of the options of developing teachers´ pedagogical content knowledge is systematic qualified reflection on their own activity. One of the way to systematic reflection can become a collective reflection with the support of video lessons. The starting point of this paper is Lesson study (a form of workshop based on the traditional organization of Japanese lessons) and finding the way how a similar model could be used in our educational culture.
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