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HPLC in quality control of food supplements based on curcumine extracts
Stříbrná, Veronika ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Analytical Chemistry Candidate: Veronika Stříbrná Supervisor: prof. RNDr. Dalibor Satinsky, Ph.D. Title of diploma thesis: HPLC in quality control of food supplements based on curcumin extracts In this diploma thesis, a new HPLC method for the simultaneous determination of active components in food supplements, piperine, curcumin, demethoxycurcumin and bisdemethoxycurcumin was developed and validated. The method was used to determine the level of the relevant analytes in the selected food supplements, namely Curcumin - piperine plus Vieste, Curcumin-piperine complex Natural Medicaments, Turmeric: piperine Setaria, Cur-Cum Astina, Curcumin Liftea, Curcumin Advance a Curcumin turmeric. Finally, the obtained results were compared with the declared extract content and evaluated as a percentage of quality of the food supplement. An Ascentis® Express RP-Amide 150 x 4,6 mm, 2,7 µm particle size analytical column with Ascentis® RP-Amide 5 x 4,6 mm, 2,7 µm particle size precolumn was used for analysis. A gradient elution with analysis time of 12.5 minutes and flow rate of mobile phase 1.0 ml/min was used. As the mobile phase a mixture of acetonitrile 100% and 0.1% phosphoric acid was chosen. The detection was performed with a DAD...
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HPLC in quality control of food supplements containing gingerols and curcumins
Hujová, Eliška ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Eliška Hujová Supervisor: prof. RNDr. Dalibor Šatínský, Ph.D. Title of the diploma thesis: HPLC in quality control of food supplements containing gingerols and curcumins In this diploma thesis, a new HPLC method for determination of 6-, 8- and 10-gingerol, 6-shogaol, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and piperine was developed and validated. The developed method was used for the analysis of food supplements: Kurkumin - piperin plus (+ zázvor) from VIESTE, Bio tumeric + ginger from VANAVITA, Ginger root extract from BioMedical, Shogaol zázvor from EPIGEMIC®, Ginger root extract from SWANSON®, and Ginger root (Zingiber officinale) from SOLGAR®. Just as a matter of interest, this method was applied for the selected tea blends containing ginger and/ or tumeric: Dilmah Orange & Ginger (flavoured ceylon black tea), Loyd Brusinky & Zázvor, Teekanne Dýchací cesty a krk se zázvorem, Pickwick Nos a krk se zázvorem, Tesco Ginger & Lemon, Teekanne Ginger Curcuma. For the analysis YMC-Triart C18 ExRS (150 x 4.6 mm; 3 µm) analytical column was used. Separation was carried out by gradient elution with mobile phases A: acetonitrile, B: ultrapure water at flow rate 1 ml/min. Detection was...
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Nano-fibers as a modern extraction sorbents for extraction of samples in chromatographic analysis
Raabová, Hedvika ; Šatínský, Dalibor (advisor) ; Ševčík, Juraj (referee) ; Ventura, Karel (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training workplace Department of Analytical Chemistry Doctoral degree program Pharmaceutical Analysis Candidate Mgr. Hedvika Raabová Supervisor prof. RNDr. Dalibor Šatínský, Ph.D. Advisor assoc. prof. PharmDr. Lucie Chocholoušová Havlíková, Ph. D. Title of Doctoral Thesis Nanofibers as modern extraction sorbents for sample extraction in chromatographic analysis Extraction techniques represent one of the most common approaches to sample handling. The analytes of interest can be isolated easily even from complex matrices, especially by solid-phase extraction (SPE). Therefore, this technique became very popular and routinely used in laboratories. The variety of commercially available sorbents has made this method even more attractive. Nevertheless, the new sorbent materials are still looked for because of the broadening spectrum of analyzed substances and the increasing demands on selectivity and extraction efficiency. Nanomaterials have been increasingly associated with these properties over the last ten years. Therefore, they are receiving more and more attention related to extraction procedures. The dissertation thesis is focused on the application of nanofibrous polymers as innovative sorbents for solid-phase extraction. First, the...
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Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis systém
Zouharová, Monika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Monika Zouharová Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis system In this diploma thesis, we tested the use of various nanofibers as sorbents for solid phase extraction of neonicotinoids connected with sequential injection analysis system. Seven types of nanofibers were tested, specifically polyamide (PA6), polycaprolactone (PCL), polyimide (PID) and polydopamine coated polyamide (PA6)coated, polycaprolactone (PCL)coated, polyimide (PID)coated and a combination of polyimide and polycaprolactone (PID/PCL)coated. These nanofibers were produced by electrospinning. The studied nanofibers were placed in the 3D printed holder, which was connected into sequential injection analysis system. UV spectrophotometry was used as a detection method. The substances to analyse were neonicotinoid insectisides. The retention of these analytes was examinated with all seven types of nanofibers. Retention of the analytes on the tested nanofibers depending on their chemical structures and physical-chemical properties was studied in these measurements. The most experiments were performed with polydopamine coated...
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Testing of Chromatographic Qualities of Alternative Stationary Phases
Havlátová, Michaela ; Šatínský, Dalibor (advisor) ; Nováková, Lucie (referee)
1 Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Havlátová Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Diploma Thesis Title: Testing of Chromatographic Qualities of Alternative Stationary Phases Chromatographic qualities of HS F5 column were tested at selected groups of substances. For testing there were chosen substances from the following groups: beta-sympatholytics (acebutolol, bopindolol), neuroleptics (thioridazine, perphenazine), local anesthetics (lidocaine, prilocaine), tetracyclins (oxytetracyclin, minocyclin), methylxanthine derivates (caffeine, theophylline) and aniline. During the analysis the retention of the tested substances was investigated as influenced by the character of the mobile phase. Some of the substances showed U-shaped retention. Three series of analysis were carried out at each tested group. In analysis number 1 acetonitrile - water was used as the mobile phase, in analysis number 2 the mobile phase was acetonitrile - phosphoric acid 0.085%, and in analysis number 3 it was acetonitrile - acetate buffer solution pH 7. In each analysis the acetonitrile percentage in the mobile phase was changed. In the group of the substances tested the U-shaped retention was shown by beta-sympatholytic...
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Using of sequential injection chromatography method for separation and determination of salicylic acid and triamcinolone acetonide in pharmaceutical preparation Triamcinolon-IVAX
Holík, Pavel ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Using of sequential injection chromatography method for separation and determination of salicylic acid and triamcinolone acetonide in pharmaceutical preparation Triamcinolon-IVAX Pavel Holík Abstract A novel and fast simultaneous determination of triamcinolone acetonide (TCA) and salicylic acid (SA) in topical pharmaceutical formulations by sequential injection chromatography (SIC) as an alternative to classical high performance liquid chromatography (HPLC) has been developed. A recently introduced Onyx™ monolithic C18 (50 mm × 4,6 mm, Phenomenex® ) with 5 mm monolithic precolumn were used for the first time for creating sequential injection chromatography system based on a FIAlab® 3000 with a six-port selection valve and 5,0 ml syringe pump in study. The mobile phase used was acetonitrile/water (35:65, v/v), pH 3.3 adjusted with acetic acid at flow rate 15 µl.s-1 . UV detection provided by fibre-optic DAD detector was set up at 240 nm. Propylparaben was chosen as suitable internal standard (IS). There is only simple pre-adjustment of the sample of topical solution (dilution with mobile phase) so the analysis is not uselessly elongated. Parameters of the method showed good linearity in wide range, correlation coefficient >0,999; system precision (relative standard deviation, R.S.D.) in the range 0,45-1,95%...
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HPLC Determination of Lutein, Zeaxanthine and Betacarotene in Dietary Products
Dvořáková, Petra ; Šatínský, Dalibor (advisor) ; Chocholoušová Havlíková, Lucie (referee)
This rigorous thesis deals with modification of method for separation carotenoids lutein, betacarotene and zeaxanthin borrowed from diploma thesis (Optimization of chromatographic conditions for HPLC determination of chosen carotenoids, Petra Dvořáková, 2009). HPLC with VIS detection is the basic method. This method was modified by gradient elution for compressed time of analysis. Four ways of gradient elution were tested during optimization of new procedure. The second aim of this rigorous thesis is summary of extraction ways for carotenoids published in special literature and proposition of extraction procedures for carotenoids from dietary products. Extraction with organic solvents was used during searching for optimal way for extraction. Simple extraction with organic solvent was tested as basic method, but extraction with previous modification of sample (saponification or acidification of extraction medium) was tested too. In spite of testing of many various ways for extraction and many various organic solvents, there wasn't find optimal and universal extraction way.
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