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Effect of intravenously applicated lactoferine on early faze; of myocardial ischemia II. Biochemical examinations
Písaříková, Veronika ; Semecký, Vladimír (advisor) ; Mladěnka, Přemysl (referee)
Lactoferrin (Lf) is an 80-kDa iron-binding glykoprotein. Lf is present physiologically in exocrine secretions, eg. tears, saliva, milk, sinovial fluid, seminal fluid and in the secondary granules of neutophils. The precise function of Lf in organism is considered to be very complex and it is still a hot subject of scientific disputation. Lf was documented to act as antimicrobial, antiinflamatory and antitumoral agent. Another of its properties is ability to inhibit hydroxyl radical formation via Fenton reaction. Afinity of Lf to iron is about 260times higher than that of transferrin. Isoproterenol (ISO) has been used as a model compound to induce infarct-like lesions in the rat and various other animal species. Administration of necrotic dose of ISO caused ischaemia followed by damage of the myocardium. Ischaemia alters iron homeostasis and redox-active free iron, which catalyses ROS-generation. The only possibility for myocardial tissue recovery in acute moycardial infarction represents the reperfusion of ischemic myocardium. But the whole process is associated with damage of myocardium due to burst of hydroxyl radical catalyzed by free iron (Fenton reaction). In our experiment we studied the effects of Lf in a catecholamine model of myocardial injury. We had four experimental groups divided according to...
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New iron chelators and antioxidants in acute myocardial model / / infarction and oxidative stress-induced catecholamine / / - effect on the basic biochemical parameters
Mladěnka, Přemysl ; Hrdina, Radomír (advisor) ; Geršl, Vladimír (referee) ; Patočka, Jiří (referee)
I. SUMMARY Background: Iron is an essential element necessary for many physiological processes involving oxygen transport, DNA-synthesis and ATP-formation. The fate of iron in the organism is tightly regulated especially at the absorption and distribution level probably mainly due to lack of specific active iron excretion mechanism. Any derangement of iron homeostatis may lead to appearance of free (unbound or loosely bound) iron, which can catalyse reactive oxygen species (ROS) production by Haber-Weiss chemistry. Cardiovascular diseases, particularly coronary heart disease (CHD), remain notwithstanding recent scientific advances important therapeutic problem. The most serious form of CHD represents acute myocardial infarction (AMI). The pathophysiology of AMI involves in most cases initial ischaemic period caused by coronary blood flow derangement due to a thrombus formation. Ischaemia alters substantially tissue homeostasis with subsequent cytosolic free iron appearance. Reconstitution of coronary blood flow (reperfusion) represents the only way for myocardial tissue recovery although on the other hand, it is linked with a release of free redox-active iron in the circulation and formation of ROS both intracellularly as well extracellularly. Iron chelators are a large group of drugs with very...
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Characterisation of multidrug resistant Klebsiella pneumoniae and Enterococcus faecium isolates by spectroscopic and genotypic method
Bavlovič, Jan ; Červený, Lukáš (advisor) ; Mladěnka, Přemysl (referee)
Charles University in Prague Faculty of Pharmacy Hradec Králové Department of Pharmacology and Toxicology Student: Jan Bavlovič Supervisors: PharmDr. Lukáš Červený, Ph.D. Prof. Doutora Luísa Peixe Co-advisors: Doutora Ângela Novais Doutora Ana Freitas Title: Characterisation of multidrug resistant Klebsiella pneumoniae and Enterococcus faecium isolates by spectroscopic and genotypic methods. Ever increasing antimicrobial resistance is currently a worldwide problem, traditionally addressed by DNA-based approaches. This study aimed to evaluate the potential of Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) for the characterization of multidrug resistant carbapenemase-producing K. pneumoniae and E. faecium isolates. We analysed 20 clinical K. pneumoniae isolates obtained from different community laboratories from Portugal between March 2014 and September 2015 and 143 previously characterized vancomycin-resistant E. faecium isolates obtained from humans, animals, and the environment in 26 countries between 1992 and 2015. Isolates were primarily characterized by genotypic methods including antimicrobial susceptibility testing, detection of carbapenemases and extended-spectrum β-lactamases (ESBLs), identification of antibiotic resistance coding transposons (Tn) and...
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