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Preparative diastereomeric separation of silybin and its sulfates by RP-HPLC
Kolářová, Petra ; Tesařová, Eva (advisor) ; Kalíková, Květa (referee)
Silymarin is standardized extract isolated from fruits of Milk Thistle plant (Silybum marianum). The principal component of silymarin is silybin. This flavonolignan is mainly responsible for the medicinal effects of Milk Thistle fruits: antioxidant, hepatoprotective, anticancer and chemoprotective activities. Natural silybin exists as an equimolar mixture of diastereomers A and B whose preparative separation is very hard. It was shown that the biological activity of silybin A and B are different. Silybin in the blood conjugates mainly to sulfates. The structure or biological activity of the sulfates is not yet known. The aim of this work is to develop practically applicable method for preparative separation of diastereomers of silybin A and B, and sulfates, which are considered as one of the major metabolites of silybin. The preparative method for separation of silybin A and B in the mobile phase consisting of 50% MeOH on the chromatographic column Labio C18 25x250 mm, was developed. In addition, preparative method for separation of mixture of products accured from the sulphation of silybin-23-acetate in the mobile phase consisting of MeOH/H2O 60/40 (v/v) with addition of 10 ml/l HCOOH, was optimized.
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"Self-portrait in the circles of smoke": self-fashioning of the modern artist as a smoker in the Czech art at the turn of the 19th and 20th century
Kolářová, Petra ; Konečný, Lubomír (advisor) ; Lahoda, Vojtěch (referee)
The topic of this Diploma Thesis is the representation of an artist as a smoker in the Czech Art at the turn of the 19th and 20th century. The motive is studied in the works of artists of the 1890s generation and the early avant-garde (Osma, Skupina výtvarných umělců), with the focus on the iconography of a smoker in art as well as the cultural history of smoking. Apart from paintings and graphics it uses other visual sources such as portrait photographs, caricatures published in contemporary newspapers and posters. The Thesis looks for a wider tradition in the European art and maps specific types of artist-smoker for whom cigarette, pipe or cigar is a part of his personal stylization. The first part concentrates on the early usage of the motive in the baroque painting and in the 19th century as such. The next part focuses on self-fashioning of modern artists characterized by their definition against social conventions. The attribute of smoking plays a role of a symbol of bohemian Iife, dandy astuteness, neurasthenic decadent, social or artistic revolt or emancipation of female-artists. The last part deals with the relationship of smoking to the actual art production of modern artists. Smoking is a metaphor for creativity of the symbolist poet or painter who draws the inspiration from his subjective...
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"Self-portrait in the circles of smoke": self-fashioning of the modern artist as a smoker in the Czech art at the turn of the 19th and 20th century
Kolářová, Petra ; Lahoda, Vojtěch (referee) ; Konečný, Lubomír (advisor)
The topic of this Diploma Thesis is the representation of an artist as a smoker in the Czech Art at the turn of the 19th and 20th century. The motive is studied in the works of artists of the 1890s generation and the early avant-garde (Osma, Skupina výtvarných umělců), with the focus on the iconography of a smoker in art as well as the cultural history of smoking. Apart from paintings and graphics it uses other visual sources such as portrait photographs, caricatures published in contemporary newspapers and posters. The Thesis looks for a wider tradition in the European art and maps specific types of artist-smoker for whom cigarette, pipe or cigar is a part of his personal stylization. The first part concentrates on the early usage of the motive in the baroque painting and in the 19th century as such. The next part focuses on self-fashioning of modern artists characterized by their definition against social conventions. The attribute of smoking plays a role of a symbol of bohemian Iife, dandy astuteness, neurasthenic decadent, social or artistic revolt or emancipation of female-artists. The last part deals with the relationship of smoking to the actual art production of modern artists. Smoking is a metaphor for creativity of the symbolist poet or painter who draws the inspiration from his subjective...
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Securing Obligations at Businesswoman Jana Kratochvílová
Kolářová, Pavla ; Kopřiva, Jan (referee) ; Musilová, Helena (advisor)
Bachelor´s thesis deals with the problem of securing of two non-monetary obligations for businesswoman Jana Kratochvílová in order to fulfill the obligations properly and in time. The first non-monetary obligation from the contract for work is a reconstruction of salesroom, the second non-monetary obligation is a delivery of new stationery from sales contract. In my work, ways of securing are analyzed and then the type of security, which would be in my opinion the most suitable for the businesswoman, is suggested.
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Is daily activity in known environment correlated with activity in experimental apparatus?
KOLÁŘOVÁ, Petra
This study was aimed at daily activity of common vole (Microtus arvalis). The features as trajectory and differences in body weight were measured. Trajectory during three days long activity were compared with trajectory measured in Open Field Test. The impact of gender was assessed and change in body weight was analysed. Values as trajectory, mobility and mean velocity were gauged in Phenotyper and Open Field test, measuring were done in EthoVision (Noldus). Analysing proved differences between genders in body weight change. Correlation between trajectories and impact of gender at daily activity were not proved.
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Final report about research in Sokolov post mining sites in 2013 based on agreement between ENKI ops and Institute of Soil Biology BC AV CR
Frouz, Jan ; Kolářová, P. ; Moradi, J. ; Zadinová, R. ; Janoušová, I. ; Hermová, M. ; Jarošíková, C. ; Vindušková, O.
Report summarises results of research about primary production, soil biota, soil water regime, and soil chemistry in post mining sites operated by Sokolovska Uhelna mining company based on agreement with ENKI ops.
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The history and present of the Moscow Metro
KOLÁŘOVÁ, Petra
The theme of this dissertation is the Moscow metro. A large part is devoted to the history and the actual construction, which was designed by one of the most important representatives of contemporary Moscow, Lazar Kaganovich. Through this work we will visit three architecturally interesting stations, go through the period of Second World War when the metro was hiding not only for the residents of Moscow, learn what is hidden under the name Metro 2 . Pay attention to some selected terrorist attacks and their consequences. At the very end, we will focus on the present and compare what happened over seventy-eight years of underground changed. This dissertation includes photos of metro stations.
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The mobbing and its interaction in the formation in the employment
KOLÁŘOVÁ, Petra
My thesis deals with the workplace violence as a serious risk factor of the daily life and work and it is a complex problem, especially when psychological violence at the work is a serious problem that is still very underrated. The harassment is the summary name that referred to very bad situation for everybody and it projects to the everyday situations at work and it is manifested not only in labor relations, but also on output and performance of individuals and whole. The thesis of the topic was chosen on the based my own experiences with the regards to the timeliness of this social problem. The main aim is to inform about the hidden and dangerous process of the psychological abuse in the workplace, many participants cannot solve it, because they are designed only for the consequences not the causes of the investigated phenomenon.
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Strategic Analysis of Rašelina, a.s.
Kolářová, Pavlína ; Dvořák, Jiří (advisor) ; Matouš, Jaroslav (referee)
The Strategic Analysis prepared for Raselina a.s. is a graduation thesis, which concentrates on theory, methodology and work experience applied to the strategy and operating management of the company. The theoretical part focuses on general methods and characteristics of strategic analysis. The work experience part summarizes the theoretical and methodological analyses and applies them to analyze the actual internal and external strategy of the company. Based on the results the SWOT analysis is provided. The conclusion is the recap of the results of all analyses and suggestion for the most effective strategy for the company.
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