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Principles of administrative proceedings
Habovčík, Martin ; Kopecký, Martin (advisor) ; Balounová, Jana (referee)
Principles of administrative proceedings, abstract Focus of this diploma thesis is the issue of basic principles of administrative proceedings, but not in terms of basic procedure principles of administrative authorities as they are set out in § 2 to 8 of the Administrative Procedure Code, but in terms of procedural principles of administrative proceedings, as rules of procedure before administrative authorities. These principles represent a key element for understanding and interpretation of the procedural process in an administrative proceeding, but Administrative Procedure Code does not contain an express codification of these principles in its text. Goal of this thesis is to perform a detailed analysis of individual principles both individually and in context. The aforementioned principles of administrative proceedings usually occur in the form of mutually ambivalent pairs and their application during administrative proceedings presents many conflicting issues which stems from their implicitly general nature. The methodological basis of this thesis is the method of textual hermeneutics and general empirical-analytical paradigm based on the source material, which consists mainly of judgments and resolutions of the Supreme Administrative Court and is supplemented by relevant literature. The text of this...
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Identification and characterisation of novel mechanisms regulating steady state and emergency granulopoiesis
Daněk, Petr ; Alberich Jorda, Meritxell (advisor) ; Fuchs, Ota (referee) ; Balounová, Jana (referee)
ABSTRACT Neutrophils are essential cells of the immune system. They engage in pathogen clearance, inflammatory response, and wound healing. Proper production and activation of neutrophils is critical for the health of an individual, since several disorders are related to neutrophilic alterations. In this thesis, we explore three previously uncharacterized mechanisms that might be involved in the regulation of neutrophilic differentiation. First, we addressed the role of the canonical Wnt signaling pathway. This signaling is executed by interaction of -catenin with TCF/LEF transcription factors. We employed a murine model that specifically inactivates -catenin-TCF/LEF-mediated transcription by expressing a dominant negative form of TCF4 (dnTCF4). Using this model in combination with several in vitro and in vivo assays we demonstrated that -catenin-TCF/LEF signaling directly upregulates expression of G-CSF receptor in hematopoietic progenitors, imposing myeloid commitment and favoring neutrophilic differentiation. This appeared to be especially important during the response to systemic infection, termed emergency granulopoiesis, as dnTCF4-expressing mice showed high susceptibility to Candida albicans infection. Remarkably, the critical role of -catenin-TCF/LEF signaling for neutrophil differentiation...
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Legal concepts of administrative law restricting the right to property
Balounová, Jana ; Kopecký, Martin (advisor) ; Staša, Josef (referee) ; Průcha, Petr (referee)
Legal concepts of administrative law restricting the right to property Within the legal order a lot of legal concepts can be found which restrict fundamental constitutionally guaranteed right to property, respectively which can restrict it. The dissertation focuses on analysis of selected legal concepts which are regulated by administrative law and through which is interfered with the right to property. In the Czech Republic the right to property is guaranteed by the Charter of Fundamental Rights and Freedoms and at European level by the Protocol to the Convention for the Protection of Human Rights and Fundamental Freedoms. The thesis is divided into three main chapters which are further divided into individual sub-chapters. The first chapter deals with the characteristic, definition and categorization of particular legal concepts restricting the right to property. The second chapter focuses on the right to property itself, especially on constitutional basis, limits and its protection. In the relation to the topic of the dissertation it is also important to define how these legal concepts are enshrined in the legal order and which form of administrative activities they result in. The third chapter describes the particular forms of administrative activities in relation to the right to property. The...
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Regulatory mechanisms in normal and malignant granulopoiesis
Kardošová, Miroslava ; Alberich Jorda, Meritxell (advisor) ; Stopka, Tomáš (referee) ; Balounová, Jana (referee)
Neutrophils, known primarily as key players in defense against invading pathogens, represent an essential component of both the innate and adaptive immunity. Continuous production of large quantities of neutrophils is ensured by a complex process termed granulopoiesis. In order to maintain a stable neutrophilic population, granulopoiesis requires to be tightly regulated. Moreover, impaired granulopoiesis may lead to aberrant bone marrow function and, ultimately, give rise to acute myeloid leukemia (AML). Despite decades of research, the mechanisms regulating granulopoiesis are still unclear. In particular, the CCAAT/enhancer binding protein (C/EBP) family of transcription factors plays a critical role in this process. C/EBPα acts as a master regulator of granulopoiesis mainly by orchestrating expression of its target genes, which will mediate granulocytic differentiation. Thus, characterization of novel C/EBPα target genes is critical for a better understanding of the molecular mechanisms that regulate granulopoiesis. Previously, we showed that another C/EBP member, CEBPG, is a direct target of C/EBPα. In the first part of the present work, we addressed the unknown role of C/EBPγ in granulopoiesis. We observed that Cebpg conditional knockout (KO) mice, which have the Cebpg gene ablated specifically...
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Tumor in vitro chemosensitivity and resistance assays (CSRA) using flow cytometry
Drozdová, Tereza ; Drbal, Karel (advisor) ; Balounová, Jana (referee)
In vitro chemosensitivity and resistance assay determine the sensitivity of a specific tumor after a specific treatment administration in an experimental setup. A heterogeneous population of cancer cells is exposed to various approved anticancer drugs in short-term ex vivo and their combination thereof. The effect of each drug is then determined based on the viability of specific tumor cells allowing for individual patient treatment using a precise combination of drugs. This approach is an example of the personalized medicine principle, which is focusing on the adjustment of diagnostic procedures and treatment of a specific patient. Therefore, its goal is to avoid treatment failure in patients with poor response to the statistically most effective treatments based on randomized clinical trials. The number of viable cells determined by the flow cytometry provides very accurate statistics for multiparametric analysis. A necessary prerequisite is the presence of dissociated cancer cells in a single cell suspension. This is different from cloning methods, where tumor colonies grow on agar media, or from histocultures, which are specific with its three-dimensional tissue cultivation. We can also sort cells from suspension based on their pre-defined attributes for their subsequent functional testing. The...
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Influence of fetal microchimerism on breast carcinoma in mothers
Šůchová, Anna-Marie ; Hromadníková, Ilona (advisor) ; Balounová, Jana (referee)
Fetal microchimerism is a condition where fetal cells are present in the body of the mother, they are transmitted during pregnancy and may persist for several decades after parturition. Their presence affects the immune system of the mother, which is investigated in both autoimmune and tumor diseases. This work, as a literature review, summarizes the current knowledge of the effect of fetal microchimerism on breast carcinomas in mothers, which is one of the most common cancer in women worldwide. It is based on studies that examined the presence of fetal cells in peripheral blood and neoplastic maternal tissues. While the correlation between the presence of fetal microchimerism in peripheral blood and breast cancer suggests a possible protective role, the data on the investigation of the same correlation in neoplastic tissue are not so unambiguous. Some of them suggest a protective role, others have a negative role. Key words - fetal microchimerism, breast carcinoma, pathogenesis, oncology, tumor
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Toll like receptors and myeloid cells in development and disease
Balounová, Jana ; Filipp, Dominik (advisor) ; Špíšek, Radek (referee) ; Vannucci, Luca Ernesto (referee)
Toll like receptors (TLRs) are germline-encoded pattern recognition receptors (PRRs) that play a central role in host cell recognition and responses to pathogens. Primarily they are responsible for induction and regulation of the innate and adaptive immune responses whereby the effector function is executed chiefly by differentiated myeloid cells. Somewhat unexpectedly, TLRs have been also shown to be involved in direct pathogen sensing by bone marrow-derived hematopoietic stem cells (HSCs) and hematopoietic progenitors when, under inflammatory conditions, the rapid generation of innate immune effector cells that effectively combat the infection is of utmost priority. While it has been recognized that the release of inflammatory cytokines from inflamed tissues along with the changes in proportions of differentiating cells in the bone marrow (BM) as well as the BM niche can nudge the differentiation of adult BM-derived cells towards myeloid cells and granulocytes, a direct role of TLRs expressed by HSCs in this process has been demonstrated only recently. However, whether a similar mechanism operates also during embryonic hematopoiesis is unknown. Here we show that TLRs and their adaptor proteins are functionally expressed during early stages of embryogenesis by short-lived maternally-transferred...
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Legal concept of public use with focus on its application in the central Pilsen borough in conflict with freedom of assembly and right to petition
Balounová, Jana ; Kopecký, Martin (advisor) ; Rajchl, Jiří (referee)
The rigorosum thesis focuses on the analysis of concept of public use, how it works in practice and on conflict of public use with freedom of assembly and right to petition. The objective of the thesis is to carry out comprehensive definition of legal concept of public use and to analyze how this legal concept works in central borough of Statutory City of Pilsen, this in the context of interrelation of public use and freedom of assembly and right to petition. The thesis is divided into five parts. First part deals with the definition of public use, its history, theory and nature, object of public use, its subjects, protection and also with relation of public use and property. In the second part, attention is paid to the types of public use, general public use and special public use. Third part of thesis presents theoretical definition of freedom of assembly and right to petition. Fourth part focuses on how public use works is practice, specifically in the central Pilsen borough. Furthermore, this part deals, inter alias, with tasks and procedures of public administration, obligations of applicant for a permit of special public use or specifics which applies to central Pilsen borough. Last part of the thesis is devoted to interrelation, respectively conflict of public use and freedom of assembly and...
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